A5030121240- Estrogen and related hormone effects
- Adipose Tissue and Metabolism
- Toxic Organic Pollutants Impact
- Retinoids in leukemia and cellular processes
- Thyroid Disorders and Treatments
- Neuroscience of respiration and sleep
- Mitochondrial Function and Pathology
- Neuroscience and Neuropharmacology Research
- Effects and risks of endocrine disrupting chemicals
- Bone Metabolism and Diseases
- Connective tissue disorders research
- Growth Hormone and Insulin-like Growth Factors
- Scoliosis diagnosis and treatment
- Ubiquitin and proteasome pathways
- Metabolism and Genetic Disorders
- Neuroendocrine regulation and behavior
- Ion Transport and Channel Regulation
- Endoplasmic Reticulum Stress and Disease
- Vitamin K Research Studies
- Pancreatic function and diabetes
- Cellular transport and secretion
- Signaling Pathways in Disease
- Receptor Mechanisms and Signaling
- Drug Transport and Resistance Mechanisms
- Trace Elements in Health
Takasaki University of Health and Welfare
2015-2025
Gunma University
2013-2024
Integrative Medicine Institute
2013
Goethe University Frankfurt
2010
Japan Science and Technology Agency
2002-2005
Ludwig Cancer Research
2002-2004
University of Shizuoka
1992-1996
Institute of Endocrinology
1990
The cDNA of a Ca(2+)-binding protein regucalcin was cloned from rat liver library which constructed in lambda ZAPII by immunoscreening. Positive clones were obtained spanned the region interest, and they gave sequence 1.7 kb sequencing with dideoxynucleotide method. Analysis showed that encoded complete amino acid molecule. Regucalcin composed 299 residues its molecular weight estimated to be 33,388 Da. hydropathy profile highly hydrophilic character. nucleotide sequences did not have...
Addition of ubiquitin or chains to target proteins leads their mono- polyubiquitination, respectively. Whereas polyubiquitination targets for degradation, monoubiquitination is thought regulate receptor internalization and endosomal sorting. Cbl are major ligases that promote ligand-dependent degradation tyrosine kinases. They also recruit CIN85-endophilin in the complex with activated receptors, thus controlling endocytosis. Here we show adaptor protein CIN85 its homologue CMS...
Polybrominated diphenyl ethers (PBDEs) have been used as flame retardants and are becoming a ubiquitous environmental contaminant. Adverse effects in the developing brain of great health concern.We investigated effect PBDEs/hydroxylated PBDEs (OH-PBDEs) on thyroid hormone (TH) receptor (TR)-mediated transcription TH-induced dendrite arborization cerebellar Purkinje cells.We examined PBDEs/OH-PBDEs TR action using transient transfection-based reporter gene assay. TR-cofactor binding was...
The distribution and expression of mRNA encoding the Ca(2+)-binding protein regucalcin in rats were investigated by Northern blot analyses. Liver cDNA (0.6 kb) was used as a probe. analyses total RNAs extracted from various tissues rat indicated that mainly present liver but only slightly kidney with size 1.8 kb. level decreased increasing age (3, 10 25 weeks). A single intraperitoneal administration calcium chloride (15 mg Ca/100 g body weight) induced remarkable increase liver; about 200%...
Protein tyrosine kinase Pyk2 and multifunctional adaptor protein Cbl are implicated in the regulation of cytoskeleton several cell types. We report that form a signaling complex is translocated to lipid rafts enriched growth cones differentiating PC12 cells following factor stimulation. found interacted with ArgBP2, which also bound flotillin-1, component raft microdomains. These interactions contributed recruitment Pyk2/Cbl compartments. In addition, Pyk2, ArgBP2 were co-localized actin...
After ischemic stroke, the corresponding area contralateral to lesion may partly compensate for loss of function. We previously reported remodeling neuronal circuits in somatosensory cortex (SSC) during first week after infarction processing bilateral information, resulting functional compensation. However, underlying processes hemisphere stroke have not yet been fully elucidated. Recent studies shown that astrocytes play critical roles synaptic reorganization and compensation a stroke....
Significance Maternal child neglect is an increasingly prevalent public health issue, but the underlying biologic processes causing this phenomenon are still largely unknown. This study examines prenatal environment of mice that later exhibit neglectful behavior. We have found that, in mice, factors determining phenotype a mother present during her own fetal period. evidence maternal prolactin (PRL) could be key factor for generating nurturing behavior offspring by activating neural circuits...
Thyroid hormones play crucial roles in the development and functional maintenance of central nervous system. Despite extensive studies neural function thyroid hormones, little is known about effects hypothyroidism on behavioural traits mechanisms underlying such effects. In present study, we report an investigation congenitally hypothyroid mutant rdw rats, revealing a novel The rats were subjected to analyses as rotarod test, open field test circadian activity measurement. To determine cause...
Abstract The role of RGPR‐p117, a transcription factor, which binds to the TTGGC motif in promoter region regucalcin gene, cell regulation remains be investigated. This study elucidated whether RGPR‐p117 regulates activity triple‐negative human breast cancer MDA‐MB‐231 cells vitro. wild‐type and RGPR‐p117‐overexpressing were cultured DMEM supplemented with fetal bovine serum. overexpression suppressed colony formation growth cells. Stimulatory effects epidermal factor on blocked by...
c-Cbl physically interacts with TrkA by anti bait coimmunoprecipitation (View interaction) Protein tyrosine kinases are known to be implicated in the majority of cellular functions, especially those involved intracellular signal transduction, whereas deregulation is often associated human diseases such as cancer [1, 2]. Binding growth factors receptor (RTKs) promotes dimerization and subsequent activation [3], which enhances autophosphorylation RTKs, phosphorylation numerous proteins,...
Aerobic (sub lactate threshold; sub-LT) exercise training facilitates oxidative phosphorylation and glycolysis of skeletal muscle. Thyroid hormone (TH) also such metabolic events. Thus, we studied whether TH signaling pathway is activated by treadmill training. Male adult rats received 30 min/day with different intensity for 12 days. Then plasma thyrotropin (TSH) levels were measured. By levels, divided into stationary control (SC, 0 m/min), sub-LT (15 m/min) supra threshold (supra-LT; 25...
The effect of signal transduction-related factors was investigated to clarify the expression mechanism for mRNA hepatic Ca(2+)-binding protein regucalcin in liver rats. change levels analyzed by Northern blotting using cDNA (0.6 kb). A single intraperitoneal administration calcium chloride (15 mg Ca2+: 0.374 mmol/100 g body weight) rats induced a remarkable increase liver; level about 170% controls at 30 min after administration. This completely inhibited simultaneous trifluoperazine (5.0...
Abstract Polychlorinated biphenyls (PCBs) are known as environmental pollutants that may cause adverse health effects. Although some congeners have been shown to affect brain development or function, the molecular mechanisms mediating their toxicity not yet fully understood. Since signal transduction via intracellular Ca 2+ is crucial for neuronal and plasticity, we investigated effect of PCBs on homeostasis membrane potential in cultured mouse cortical cells. Acute exposure hydroxylated PCB...
To study the role of thyroid hormone (TH) in cerebellar development, we generated transgenic mice expressing a dominant-negative TH receptor (TR) Purkinje cells. A mutant human TRβ1 (G345R), which binds to TH-response element but cannot bind T3, was subcloned into exon 4 full-length L7/Pcp-2 gene, is specifically expressed and retinal rod bipolar The transgene cells postnatal cerebellum. cell dendrite arborization significantly delayed mice. Surprisingly, granule migration also delayed. In...
Abstract Early‐life stress induces several neuropsychological disorders in adulthood, including depression. Such may be induced by functional alteration of the glutamatergic system. However, their underlying mechanisms have not yet been fully clarified. Furthermore, involvement glucocorticoids, which are representative hormones, has In this study, we used maternal deprivation ( MD ) mice as an early‐life‐stress model, and studied changes system adulthood. The glutamate concentration neuronal...
The inhibitory effect of beta-alanyl-L-histidinato zinc (AHZ) on bone resorption in tissue culture was investigated. Calvaria were removed from weanling rats (3-week-old male) and cultured for periods up to 48 h Dulbecco's modified Eagle medium (high glucose, 4.5%) supplemented with antibiotics bovine serum albumin. experimental cultures contained 10(-7) 10(-4) mol/l AHZ. bone-resorbing factors, parathyroid hormone (1-34) (PTH; mol/l), prostaglandin E2 (10(-5) interleukin-1 alpha (IL1 alpha;...