A5103131697- RNA Research and Splicing
- Genomics and Phylogenetic Studies
- COVID-19 diagnosis using AI
- RNA and protein synthesis mechanisms
- Tryptophan and brain disorders
- Stress Responses and Cortisol
- Genetic diversity and population structure
- Salmonella and Campylobacter epidemiology
- Neuroscience and Neuropharmacology Research
- RNA modifications and cancer
- Chromosomal and Genetic Variations
- Cardiovascular Disease and Adiposity
- Probiotics and Fermented Foods
- Avian ecology and behavior
- T-cell and B-cell Immunology
- Cancer-related gene regulation
- TGF-β signaling in diseases
- Salivary Gland Disorders and Functions
- Viral Infectious Diseases and Gene Expression in Insects
- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- Moyamoya disease diagnosis and treatment
- Identification and Quantification in Food
- Cerebrovascular and Carotid Artery Diseases
- Gene expression and cancer classification
Environment and Climate Change Canada
2022-2024
Centers for Disease Control and Prevention
2016-2023
National Center for Emerging and Zoonotic Infectious Diseases
2023
Ragon Institute of MGH, MIT and Harvard
2019
Georgia Institute of Technology
2009-2018
University of Manitoba
2017
IST Research
2010
Atlanta Technical College
2009
St Mary's Hospital
1996
Marshfield Clinic
1993
Young Indigenous children in North America suffer from a higher degree of severe early childhood caries (S-ECC) than the general population, leading to speculation that etiology and characteristics disease may be distinct this population. To address knowledge gap, we conducted first microbiome analysis an population using modern molecular techniques. We investigated caries-associated among Canadian First Nations with S-ECC. Thirty <72 mo age S-ECC 20 caries-free were recruited Winnipeg,...
ABSTRACT Diagnostic testing for foodborne pathogens relies on culture-based techniques that are not rapid enough real-time disease surveillance and do give a quantitative picture of pathogen abundance or the response natural microbiome. Powerful sequence-based culture-independent approaches, such as shotgun metagenomics, could sidestep these limitations potentially reveal pathogen-specific signature microbiome would have implications only diagnostics but also better understanding progression...
Identification of enteric bacteria species by whole genome sequence (WGS) analysis requires a rapid and an easily standardized approach. We leveraged the principles average nucleotide identity using MUMmer (ANIm) software, which calculates percent bases aligned between two bacterial genomes their corresponding ANI values, to set threshold values for determining consistent with conventional identification methods known species. The performance was evaluated datasets: Reference Genome Dataset...
Abstract When a single species evolves into multiple descendent species, some parts of the genome can play key role in evolution reproductive isolation while other flow between evolving via interbreeding. Genomic during speciation process is particularly interesting when major components genome—for instance, sex chromosomes vs. autosomes mitochondrial DNA—show widely differing patterns relationships three diverging populations. The golden-crowned sparrow (Zonotrichia atricapilla) and...
Three distinct isoforms of transforming growth factor beta (TGF-beta) are expressed in mammalian cells. Although many cells respond equivalently to all three isoforms, certain selectively. Using chimeric proteins which selected regions the different were interchanged, we have identified two functional domains TGF-beta involved determining biological potencies and functions molecule. The first domain is important for whether can be sequestered by alpha 2-macroglobulin. By replacing aa 45 47 2...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of disease 2019 (COVID-19), has spread globally and is being surveilled with an international genome sequencing effort. Surveillance consists sample acquisition, library preparation, whole sequencing. This necessitated a classification scheme detailing Variants Concern (VOC) Interest (VOI), rapid expansion bioinformatics tools for sequence analysis. These bioinformatic are means major actionable results: maintaining...
The control of cytoskeletal dynamics by dedicator cytokinesis 2 (DOCK2), a hematopoietic cell-specific actin effector protein, has been implicated in TCR signaling and T cell migration. Biallelic mutations Dock2 have identified patients with recessive form combined immunodeficiency defects T, B, NK activation. Surprisingly, we show this study that certain immune functions CD8+ cells are enhanced the absence DOCK2. Dock2-deficient mice pronounced expansion their memory compartment. Bone...
Expression levels of the miR-200 family miRNAs are significantly reduced during epithelial-to-mesenchymal transition (EMT) and consequent metastasis ovarian other cancers. Consistently, ectopic over-expression in mesenchymal-like cells reverses process by converting treated to an epithelial phenotype, thereby reducing invasiveness increasing sensitivity chemotherapeutic drugs. To better understand dynamics molecular processes underlying miRNA-induced mesenchymal-to mesenchymal (MET), a...
Resistance genes encoding β-lactamases (BLs) confer resistance to the widely prescribed antibiotic class β-lactams. Therefore, it is important assess prevalence of BL in clinical or environmental samples for monitoring spreading these into pathogens and estimating public health risk.
Abstract Sympatric species pairs provide researchers with the opportunity to study patterns of genomic differentiation during late stages speciation and identify regions underlying reproductive isolation. The Golden-crowned Sparrow ( Zonotrichia atricapilla ) White-crowned leucophrys are broadly sympatric songbirds found in western North America. These sister phenotypically differentiated largely reproductively isolated despite possessing similar mitochondrial genomes, likely due recent...