A5041499103- Vibrio bacteria research studies
- Salmonella and Campylobacter epidemiology
- Aquaculture disease management and microbiota
- Listeria monocytogenes in Food Safety
- Genomics and Phylogenetic Studies
- Food Safety and Hygiene
- Identification and Quantification in Food
- Genetic diversity and population structure
- Escherichia coli research studies
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Yersinia bacterium, plague, ectoparasites research
- Plant and animal studies
- Animal Behavior and Reproduction
- Viral gastroenteritis research and epidemiology
- Cassava research and cyanide
- Enterobacteriaceae and Cronobacter Research
- Probiotics and Fermented Foods
- Avian ecology and behavior
- Essential Oils and Antimicrobial Activity
- Spectroscopy and Chemometric Analyses
- Zoonotic diseases and public health
- Bat Biology and Ecology Studies
- Bacterial Genetics and Biotechnology
- Genetic and phenotypic traits in livestock
Centers for Disease Control and Prevention
2013-2023
Libin Cardiovascular Institute of Alberta
2022
University of Calgary
2022
National Center for Emerging and Zoonotic Infectious Diseases
2007-2021
University of Haiti
2014
University of Maryland, College Park
2014
Public Health Agency of Canada
2011
Kenya Medical Research Institute
2011
Chinese Center For Disease Control and Prevention
2011
National Health Laboratory Service
2011
The Hawaiian Islands form as the Pacific Plate moves over a ‘hot spot’ in earth’s mantle where magma extrudes through crust to build huge shield volcanos. islands subside and erode plate carries them north‐west, eventually become coral atolls seamounts. Thus are ordered linearly by age, with oldest north‐west (e.g. Kauai at 5.1 Ma) youngest south‐east Hawaii 0.43 Ma). K–Ar estimates of date an island’s formation provide maximum age for taxa inhabiting island. These ages can be used calibrate...
Although new pathogen-vehicle combinations are increasingly being identified in produce-related disease outbreaks, fresh produce is a rarely recognized vehicle for listeriosis. We investigated nationwide listeriosis outbreak that occurred the United States during 2011.We defined an outbreak-related case as laboratory-confirmed infection with any of five subtypes Listeria monocytogenes isolated period from August 1 through October 31, 2011. Multistate epidemiologic, trace-back, and...
Listeriamonocytogenes ( Lm ) causes severe foodborne illness (listeriosis). Previous molecular subtyping methods, such as pulsed-field gel electrophoresis (PFGE), were critical in detecting outbreaks that led to food safety improvements and declining incidence, but PFGE provides limited genetic resolution. A multiagency collaboration began performing real-time, whole-genome sequencing (WGS) on all US isolates from patients, food, the environment September 2013, posting data into a public...
Wave upon wave of disease The cholera pathogen, Vibrio cholerae , is considered to be ubiquitous in water systems, making the design eradication measures apparently fruitless. Nevertheless, local and global populations remain distinct. Now, Weill et al. Domman show that a surprising diversity between continents has been established. Latin America Africa bear different variants toxin with transmission dynamics ecological niches. data are not consistent establishment long-term reservoirs...
Whole apples have not been previously implicated in outbreaks of foodborne bacterial illness. We investigated a nationwide listeriosis outbreak associated with caramel apples. defined an outbreak-associated case as infection one or both two strains Listeria monocytogenes highly related by whole-genome multilocus sequence typing (wgMLST) from 1 October 2014 to February 2015. Single-interviewer open-ended interviews identified the source. Outbreak-associated cases were compared...
ABSTRACT Vibrio , a diverse genus of aquatic bacteria, currently includes 72 species, 12 which occur in human clinical samples. Of these 12, three species— cholerae parahaemolyticus and vulnificus —account for the majority infections humans. Rapid accurate identification species has been problematic because phenotypic characteristics are variable within biochemical requires 2 or more days to complete. To facilitate human-pathogenic we developed multiplex PCR that uses species-specific...
Wave upon wave of disease The cholera pathogen, Vibrio cholerae , is considered to be ubiquitous in water systems, making the design eradication measures apparently fruitless. Nevertheless, local and global populations remain distinct. Now, Weill et al. Domman show that a surprising diversity between continents has been established. Latin America Africa bear different variants toxin with transmission dynamics ecological niches. data are not consistent establishment long-term reservoirs...
Cholera was absent from the island of Hispaniola at least a century before an outbreak that began in Haiti fall 2010. Pulsed-field gel electrophoresis (PFGE) analysis clinical isolates and recent global travelers returning to United States showed indistinguishable PFGE fingerprints. To better explore genetic ancestry strain, we acquired 23 whole-genome Vibrio cholerae sequences: 9 obtained or Dominican Republic, 12 pattern-matched linked Asia Africa, 2 nonmatched outliers Western Hemisphere....
ABSTRACT Prior to the epidemic that emerged in Haiti October of 2010, cholera had not been documented this country. After its introduction, a strain Vibrio cholerae O1 spread rapidly throughout Haiti, where it caused over 600,000 cases disease and >7,500 deaths first two years epidemic. We applied whole-genome sequencing temporal series V. isolates from gain insight into mode tempo evolution isolated population O1. Phylogenetic Bayesian analyses supported hypothesis all sample set...
Epidemiological findings of a listeriosis outbreak in 2013 implicated Hispanic-style cheese produced by company A, and pulsed-field gel electrophoresis (PFGE) whole genome sequencing (WGS) were performed on clinical isolates representative collected from A environmental samples during the investigation. The results strengthened evidence for as vehicle. Surveillance sampling WGS 3 months later revealed that equipment purchased B yielded an isolate highly similar to all isolates. core...
A multistate listeriosis outbreak associated with cantaloupe consumption was reported in the United States September, 2011. The investigation recorded a total of 146 invasive illnesses, 30 deaths and one miscarriage. Subtyping clinical, food environmental isolates revealed two serotypes (1/2a 1/2b) four pulsed-field gel electrophoresis two-enzyme pattern combinations I, II, III, IV, including rarely seen before this outbreak. DNA-microarray, Listeria GeneChip®, developed by FDA from 24...
In May of 2011, an enteroaggregative Escherichia coli O104:H4 strain that had acquired a Shiga toxin 2-converting phage caused large outbreak bloody diarrhea in Europe which was notable for its high prevalence hemolytic uremic syndrome cases. Several studies have described the genomic inventory and phylogenies strains associated with collection historical E. isolates using draft genome assemblies. We present complete, closed sequences isolate from 2011 (2011C-3493) two cases occurred...
Abstract Invasive Cronobacter infections among infants are associated with severe neurologic disabilities and death. Early reports typically featured hospitalized preterm recognized contaminated powdered infant formula (PIF) as a transmission vehicle. To clarify recent epidemiology, we reviewed all cases of bloodstream infection or meningitis that were reported to the Centers for Disease Control Prevention in literature (1961–2018; n = 183). Most neonates (100/150 [67%]); 38% (42/112) died,...
Widespread species that are morphologically uniform may be likely to harbour cryptic genetic variation. Common ravens (Corvus corax) have an extensive range covering nearly the entire Northern Hemisphere, but show little discrete phenotypic We obtained tissue samples from throughout much of this and collected mitochondrial sequence nuclear microsatellite data. Our study revealed a deep break between western United States rest world. These two groups, 'California clade' 'Holarctic well...
An analysis of restriction-site variation in mitochondrial DNA was conducted to examine relationships among five taxa one group honeycreepers-the amakihi complex (genus Hemignathus). We analyzed 35 ingroup and 3 outgroup samples. Tree topologies, based on both distance parsimony methods, grouped into two distinct lineages: the virens-wilsoni lineage; chloris-stejnegeri-parvus group. Inter-island sequence divergence (average $d_{xy}=0.0368$) is considerably higher than intra-island (mean...
We identified a novel serotype 1/2a outbreak strain and 2 epidemic clones of Listeria monocytogenes while investigating foodborne listeriosis associated with consumption cantaloupe during 2011 in the United States. Comparative analyses strains worldwide are essential to identification clones.
ABSTRACT Vibrio cholerae represents both an environmental pathogen and a widely distributed microbial species comprised of closely related strains occurring in the tropical to temperate coastal ocean across globe (Colwell RR, Science 274:2025–2031, 1996; Griffith DC, Kelly-Hope LA, Miller MA, Am. J. Trop. Med. Hyg. 75:973–977, 2006; Reidl J, Klose KE, FEMS Microbiol. Rev. 26:125–139, 2002). However, although this implies dispersal growth diverse conditions, how locally successful populations...
In October 2010, the US Centers for Disease Control and Prevention received reports of cases severe watery diarrhea in Haiti. The cause was confirmed to be toxigenic Vibrio cholerae, serogroup O1, serotype Ogawa, biotype El Tor. We characterized 122 isolates from Haiti compared them with other countries. Antimicrobial drug susceptibility tested by disk diffusion broth microdilution. Analyses included identification rstR VC2346 genes, sequencing ctxAB tcpA pulsed-field gel electrophoresis...
ABSTRACT Diagnostic testing for foodborne pathogens relies on culture-based techniques that are not rapid enough real-time disease surveillance and do give a quantitative picture of pathogen abundance or the response natural microbiome. Powerful sequence-based culture-independent approaches, such as shotgun metagenomics, could sidestep these limitations potentially reveal pathogen-specific signature microbiome would have implications only diagnostics but also better understanding progression...
ABSTRACT The bacterial pathogen Vibrio cholerae can occupy both the human gut and aquatic reservoirs, where it may colonize chitinous surfaces that induce expression of factors for three phenotypes: chitin utilization, DNA uptake by natural transformation, contact-dependent killing via a type VI secretion system (T6SS). In this study, we surveyed diverse set 53 isolates from different geographic locales collected over past century clinical environmental specimens each phenotype outlined...
The bacterial foodborne pathogen Listeria monocytogenes clonal complex 1 (Lm-CC1) is the most prevalent group associated with human listeriosis and strongly cattle dairy products. Here, we analyze 2021 isolates collected from 40 countries, covering Lm-CC1 first isolation to present days, define its evolutionary history population dynamics. We show that spread worldwide North America following Industrial Revolution through two waves of expansion, coinciding transatlantic livestock trade in...