A5073023351- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Antifungal resistance and susceptibility
- Helicobacter pylori-related gastroenterology studies
- Infectious Diseases and Mycology
- Mycotoxins in Agriculture and Food
- Pneumocystis jirovecii pneumonia detection and treatment
- Infectious Diseases and Tuberculosis
- Fungal Infections and Studies
- Tracheal and airway disorders
- Pleural and Pulmonary Diseases
- Amoebic Infections and Treatments
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Ocular Infections and Treatments
- Lung Cancer Diagnosis and Treatment
- Urinary Tract Infections Management
- Parasitic Diseases Research and Treatment
- Microscopic Colitis
- Antibiotic Use and Resistance
- Antibiotic Resistance in Bacteria
- Autoimmune Bullous Skin Diseases
- Parasitic Infections and Diagnostics
- Bacterial Identification and Susceptibility Testing
- Plant Pathogens and Fungal Diseases
Complejo Asistencial Universitario de Palencia
2021-2024
Junta de Castilla y León
2021-2024
Hospital de León
2020
Hospital El Bierzo
2009-2019
Universidad de Salamanca
1994-2000
Johns Hopkins University
1998
Baltimore VA Medical Center
1998
Institut Pasteur
1997
Consejo Superior de Investigaciones Científicas
1995-1996
Aspergillus fumigatus possesses two catalases (described as fast and slow on the basis of their electrophoretic mobility). The catalase has been recognized a diagnostic antigen for aspergillosis in immunocompetent patients. antigenic purified. enzyme is tetrameric protein composed 90-kDa subunits. corresponding cat1 gene was cloned, sequencing data show that codes 728-amino-acid polypeptide. A recombinant expressed Pichia pastoris enzymatically active biochemical properties are similar to...
Nontuberculous mycobacteria (NTM) are environmental and ubiquitous, but only a few species associated with disease, often presented as nodular/bronchiectatic or cavitary pulmonary forms. Bronchiectasis, airways dilatations characterized by chronic productive cough, is the main presentation of NTM disease. The current Cole’s vicious circle model for bronchiectasis proposes that it progresses from damaging insult, such pneumonia, affects respiratory epithelium compromises mucociliary clearance...
We have identified, purified, and characterized structurally functionally a 90-kDa immunodominant antigen associated with the water-soluble fraction of Aspergillus fumigatus. This is recognized by 90.3% serum samples from patients aspergilloma should be considered either itself or better in combination other purified antigens as candidate for developing standardized immunoassay detection aspergilloma. p90 glycoprotein containing at least two N-linked sugar chains 2 5 kDa, respectively, which...
Aspergillus nidulans catalase B (CatB) was purified to homogeneity and characterized as a hydroperoxidase which resembles typical catalases in some physicochemical characteristics: (1) it has an apparent molecular weight of 360 000 is composed four glycosylated subunits, (2) hydrophobic properties revealed by extractability ethanol/chloroform binding phenyl‐Superose, (3) acidic isoelectric point at pH 3.5. Also CatB exhibits distinctive properties, e.g. not inhibited the presence 2% sodium...
For the first time, an immunodominant Aspergillus nidulans antigen (ASPND1) consistently reactive with serum samples from aspergilloma patients has been purified and characterized, its coding gene (aspnd1) cloned sequenced. ASPND1 is a glycoprotein four N-glycosidically-bound sugar chains (around 2.1 kDa each) which are not necessary for reactivity immune human sera. The polypeptide part synthesized as 277-amino-acid precursor of 30.6 that after cleavage putative signal peptide 16 amino...
The influence of culture medium and time temperature growth on the appearance Aspergillus nidulans antigens was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed silver staining or Western blot (immunoblot), proteins present in total cellular extracts supernatants. Samples exponential, deceleration, stationary phases were selected biochemical, morphological, ultrastructural criteria. Protein antigen patterns (detected with rabbit antibodies) from very similar...
Summary: Cytosolic fractions of mycelial extracts from Aspergillus nidulans, A. flavus, and three different isolates fumigatus, grown to stationary phase in Czapek-Dox-AOAC medium, were tested by immunoblotting for the presence antigens reactive 80 serum samples aspergilloma patients. Fifty control used determine specificity reactions. In fumigatus cytosolic fraction a group four main antigenic bands (p90, p60, p40 p37) was consistently recognized (in total or partial form) 90% This...
Cutaneous fungal infections can result in disastrous episodes if improperly diagnosed and treated, especially immunosuppressed patients. Although dermatopathologists are highly familiar with some filamentous fungi – such as Aspergillus Zygomycetes they not so aware of other less common species. We report a case ocular infection by Scedosporium apiospermum that started conjunctivitis resulted Phthisis bulbi subsequent exeresis the left eye. describe main morphological features fungus well...
The objectives of this work are two: first, to evaluate the resistance Escherichia coli several antibiotics and their trends over a six-year period in strands isolated urine samples from patients receiving health-care general practitioner offices our environment; second, if empirical treatment regimens commonly accepted country would be applicable environment depending on results study.We analyzed cultures positive for obtained collected at 10 primary health care centers area El Bierzo...