A5105516523- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer-related gene regulation
- RNA Research and Splicing
- Pluripotent Stem Cells Research
- Genetic Neurodegenerative Diseases
- Histone Deacetylase Inhibitors Research
- Microtubule and mitosis dynamics
- DNA and Nucleic Acid Chemistry
- Fungal and yeast genetics research
- CRISPR and Genetic Engineering
- DNA Repair Mechanisms
- Estrogen and related hormone effects
École Polytechnique Fédérale de Lausanne
2022-2025
University of Geneva
2017-2021
University of Turin
2016
Binding of mammalian transcription factors (TFs) to regulatory regions is hindered by chromatin compaction and DNA methylation their binding sites. Nevertheless, pioneer (PFs), a distinct class TFs, have the ability access nucleosomal DNA, leading nucleosome remodelling enhanced accessibility. Whether PFs can bind methylated sites induce demethylation largely unknown. Using highly parallelized approach investigate PF demethylation, we show that interdependence between TF more complex than...
Expanded CAG/CTG repeats underlie 13 neurological disorders, including myotonic dystrophy type 1 (DM1) and Huntington's disease (HD). Upon expansion, loci acquire heterochromatic characteristics, which may provoke changes to chromatin conformation thereby affect both gene expression repeat instability. Here, we tested this hypothesis by performing 4C sequencing at the DMPK HTT from DM1 HD-derived cells. We find that allele sizes ranging 15 1700 displayed similar interaction profiles. This...
Estrogens are neuroprotective factors in several neurological diseases. Neuroglobin (NGB) is one of the estrogen target gene involved neuroprotection, but little known about its transcriptional regulation. Estrogen genomic pathway expression regulation mediated by receptors (ERα and ERβ) that bind to specific regulatory regions. We focused our attention on E2-induced NGB human differentiated neuronal cell lines (SK-N-BE NT-2). Previously, using bioinformatics analysis we identified a...
Estrogen hormones are implicated in a majority of breast cancers and estrogen receptor alpha (ER), the main nuclear factor mediating signaling, orchestrates complex molecular circuitry that is not yet fully elucidated. Here, we investigated genome-wide DNA methylation, histone acetylation transcription after estradiol (E2) deprivation re-stimulation to better characterize ability ER coordinate gene regulation. We found E2 mostly resulted hypermethylation deacetylation enhancers....
Summary The maintenance of cell identity faces many challenges during mitosis, as most DNA-binding proteins are evicted from DNA and transcription is virtually abolished. How cells maintain their through division faithfully re-initiate gene expression mitotic exit unclear. Here, we developed a novel reporter system enabling cycle synchronization-free separation pluripotent stem in temporal bins < 30 minutes exit. This allowed us to quantify genome-wide reactivation transcription,...
Abstract Access of mammalian transcription factors (TFs) to regulatory regions, an essential event for regulation, is hindered by chromatin compaction involving nucleosome wrapping, repressive histone modifications and DNA methylation. Moreover, methylation TF binding sites (TBSs) affects affinity these sites. Remarkably, a special class TFs called pioneer (PFs) can access nucleosomal DNA, leading remodelling opening. However, whether PFs bind methylated induce demethylation largely unknown....
Abstract Expanded CAG/CTG repeats underlie thirteen neurological disorders, including myotonic dystrophy (DM1) and Huntington’s disease (HD). Upon expansion, repeat loci acquire heterochromatic characteristics. This observation raises the hypothesis that expansion provokes changes to higher order chromatin folding thereby affects both gene expression in cis genetic instability of tract. Here we tested this directly by performing 4C sequencing at DMPK HTT from DM1 HD patient-derived cells....