A5068784639- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Esophageal Cancer Research and Treatment
- Prenatal Screening and Diagnostics
- Genetic Syndromes and Imprinting
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- Genomics and Chromatin Dynamics
- Reproductive Biology and Fertility
- Mycotoxins in Agriculture and Food
- Histone Deacetylase Inhibitors Research
- Bladder and Urothelial Cancer Treatments
- Viral-associated cancers and disorders
- Birth, Development, and Health
- Reproductive Health and Technologies
- Hair Growth and Disorders
- Fungal and yeast genetics research
- Extracellular vesicles in disease
- Biochemical and Molecular Research
- Wheat and Barley Genetics and Pathology
- DNA Repair Mechanisms
- Arsenic contamination and mitigation
- Cellular transport and secretion
Centre International de Recherche sur le Cancer
2019-2025
KU Leuven
2015-2024
Centre de Recherche en Biologie cellulaire de Montpellier
2013
Institut Cellule Souche et Cerveau
2011-2012
Inserm
2011-2012
Entry into mitosis is triggered by cyclinB/Cdk1, whose activity abruptly raised a positive feedback loop. The Greatwall kinase phosphorylates proteins of the endosulfine family and allows them to bind inhibit main Cdk1-counteracting PP2A-B55 phosphatase, thereby promoting mitotic entry. In contrast most eukaryotic systems, Cdc14 Cdk1-antagonizing phosphatase in budding yeast, while PP2ACdc55 promotes, instead preventing, entry participating loop Cdk1 activation. Here we show that yeast...
Abstract Epigenetic mechanisms such as aberrant DNA methylation (DNAme) are known to drive esophageal squamous cell carcinoma (ESCC), yet they remain poorly understood. Here, we studied tumor-specific DNAme in ESCC cases from nine high-incidence countries of Africa, Asia, and South America. Infinium MethylationEPIC array was performed on 108 tumors 51 normal tissues adjacent the (NAT) discovery phase, targeted pyrosequencing 132 36 NAT replication phase. Top genes for were prioritized by...
Epstein–Barr Virus (EBV) infects more than 90% of the adult population worldwide. EBV infection is associated with Burkitt lymphoma (BL) though alone not sufficient to induce carcinogenesis implying involvement co-factors. BL endemic in African regions faced mycotoxins exposure. Exposure and oncogenic viruses has been shown increase cancer risks partly through deregulation immune response. A recent transcriptome profiling B cells exposed aflatoxin B1 (AFB1) revealed an upregulation Chemokine...
The Tet 5-methylcytosine dioxygenases catalyze DNA demethylation by producing 5-hydroxymethylcytosine and further oxidized products. Tet1 Tet2 are highly expressed in mouse pluripotent cells downregulated to different extents somatic cells, but the transcriptional mechanisms unclear. Here we defined promoter enhancer domains Tet2. Within a 15-kb "superenhancer" of Tet1, there two transcription start sites (TSSs) with activation patterns during development. A 6-kb region upstream distal TSS...
Abstract STUDY QUESTION What biological processes are linked to the signaling of energy sensor 5′-AMP-activated protein kinase (AMPK) in mouse and human granulosa cells (GCs)? SUMMARY ANSWER The lack α1AMPK GCs impacted cell cycle, adhesion, lipid metabolism induced a hyperandrogenic response. WHAT IS KNOWN ALREADY AMPK is expressed ovarian follicle, its activation by pharmacological medications, such as metformin, inhibits production steroids. Polycystic ovary syndrome (PCOS) responsible...
Small intestinal neuroendocrine tumors (siNETs) are rare bowel arising from malignant enteroendocrine cells, which normally regulate digestion throughout the intestine. Though infrequent, their incidence is rising through better diagnosis, fostering research into origin and treatment. To date, siNETs considered to be a single entity clinically treated as such. Here, by performing multi-omics analysis of siNETs, we unveil four distinct molecular groups with strong clinical relevance provide...
The recent identification of recurrently mutated epigenetic regulator genes (ERGs) supports their critical role in tumorigenesis. We conducted a pan-cancer analysis integrating (epi)genome, transcriptome, and DNA methylome alterations curated list 426 ERGs across 33 cancer types, comprising 10,845 tumor 730 normal tissues. found that, addition to mutations, copy number were more frequent than previously anticipated tightly linked expression aberrations. Novel bioinformatics approaches, the...
In early mouse pre-implantation development, primitive endoderm (PrE) precursors are platelet-derived growth factor receptor alpha (PDGFRα) positive. Here, we demonstrated that cultured embryonic stem cells (mESCs) express PDGFRα heterogeneously, fluctuating between a PDGFRα+ (PrE-primed) and platelet endothelial cell adhesion molecule 1 (PECAM1)-positive state (epiblast-primed). The two surface markers can be co-detected on third subpopulation, expressing epiblast PrE determinants...
Summary To evaluate the integrity of genomic imprinting in embryos that failed to develop normally following intracytoplasmic sperm injection (ICSI), we analysed methylation profile H19 and KCNQ1OT1 control regions, H19DMR KvDMR1 respectively, high-grade blastocysts exhibited developmental anomalies. Significant hypomethylation was specifically observed 5/5 atypical graded BC, which probably reflected vulnerability imprint inner cell mass during remodelling phase early embryo. In addition,...
Metformin is a drug used for the treatment of type 2 diabetes and disorders associated with insulin resistance. also in pregnancy such as gestational diabetes. However, consequences foetal exposure to metformin on fertility exposed offspring remain poorly documented. In this study, we investigated effect utero female male offspring. We observed that detectable blood mother amniotic fluid umbilical cord. was not measurable any tissues embryo, including gonads. The sex specific. adult females...
Reprogramming somatic cells into induced pluripotent stem (iPSCs) involves the reactivation of endogenous pluripotency genes and global DNA demethylation, but temporal resolution these events using existing markers is limited. Here, we generate murine transgenic lines harboring reporters for 5-methylcytosine dioxygenase Tet1 Oct4. By monitoring dual reporter fluorescence during entry, identify a sequential order Oct4 activation by proximal distal regulatory elements. Full marks an...
Burkitt lymphoma (BL) is a malignant B cell neoplasm that accounts for almost half of pediatric cancers in sub-Saharan African countries. Although the BL endemic prevalence attributable to combination Epstein–Barr virus (EBV) infection with malaria and environmental carcinogens exposure, such as food contaminant aflatoxin B1 (AFB1), molecular determinants underlying pathogenesis are not fully understood. Consistent role epigenetic mechanisms at interface between genome environment, AFB1 EBV...
The etiology of neural tube defects (NTDs) involves complex gene-environmental interactions. Folic acid (FA) prevents NTDs, but the mechanisms remain poorly understood and at least 30% human NTDs resist beneficial effects FA supplementation. Here, we identify DNA demethylase TET1 as a nexus folate-dependent one-carbon metabolism genetic risk factors post-neural closure. We determine that cranial in
Rhabdomyosarcoma is a pediatric cancer associated with aggressiveness and tendency to develop metastases. Fusion-negative rhabdomyosarcoma (FN-RMS) the most commonly occurring subtype of RMS, where metastatic disease can hinder treatment success decrease survival rates. RMS-derived exosomes were previously demonstrated be enriched miRNAs, including miR-1246, possibly contributing aggressiveness. We aimed decipher functional impact exosomal miR-1246 on recipient cells its role in promoting...
All routine clinical treatments for colorectal cancer include 5-fluorouracil (5-FU), which cannot counteract recurrence and metastases formation. As the pyrimidine analog 5-FU can impact multiple pathways including both DNA RNA metabolism, studying its mode of actions could lead to improved therapies. Using a dedicated reporter system lineage-tracing deep translatome profiling we demonstrate that causes some cells tolerate drug, due durable translational reprogramming sustains cell...
Estrogen hormones are implicated in a majority of breast cancers and estrogen receptor alpha (ER), the main nuclear factor mediating signaling, orchestrates complex molecular circuitry that is not yet fully elucidated. Here, we investigated genome-wide DNA methylation, histone acetylation transcription after estradiol (E2) deprivation re-stimulation to better characterize ability ER coordinate gene regulation. We found E2 mostly resulted hypermethylation deacetylation enhancers....
Gastrulation in the early postimplantation stage mammalian embryo begins when epiblast cells ingress to form primitive streak or develop as embryonic ectoderm. The DNA dioxygenase Tet1 is highly expressed and yet continues regulate lineage specification during gastrulation its expression diminished. Here, we show how plays a pivotal role upstream of germ layer bifurcation. During transition from naive pluripotency priming, global reconfiguration redistributes Oct4-cobound promoters distal...