A5080289439- Cervical Cancer and HPV Research
- RNA modifications and cancer
- Cancer-related Molecular Pathways
- Cancer Research and Treatments
- Virus-based gene therapy research
- NF-κB Signaling Pathways
- Viral-associated cancers and disorders
- Herpesvirus Infections and Treatments
- Epigenetics and DNA Methylation
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- interferon and immune responses
- Cytokine Signaling Pathways and Interactions
- Nonmelanoma Skin Cancer Studies
- Cancer-related molecular mechanisms research
- Head and Neck Cancer Studies
- Axon Guidance and Neuronal Signaling
- Cancer and Skin Lesions
- T-cell and Retrovirus Studies
- Polyomavirus and related diseases
- Cancer-related gene regulation
- Hepatitis B Virus Studies
- Corneal Surgery and Treatments
- RNA Research and Splicing
- Apelin-related biomedical research
Centre International de Recherche sur le Cancer
2013-2024
German Cancer Research Center
2002-2003
University of Catania
2002
DKFZ-ZMBH Alliance
2002
Abstract Cervical cancer development is linked to the persistent infection by high-risk mucosal human papillomaviruses (HPVs) types. The E6 and E7 major oncoproteins from this dsDNA virus play a key role in deregulation of cell cycle, apoptosis, adaptive immune surveillance. In study, we show for first time that HPV type 16 (HPV16), most carcinogenic among subgroup, interferes with innate immunity affecting expression TLRs. Infection primary keratinocytes HPV16 recombinant retroviruses...
Cutaneous beta human papillomavirus (HPV) types are suspected to be involved, together with ultraviolet (UV) radiation, in the development of non-melanoma skin cancer (NMSC). Studies vitro and vivo experimental models have highlighted transforming properties HPV E6 E7 oncoproteins. However, epidemiological findings indicate that may required only at an initial stage carcinogenesis, become dispensable after full establishment NMSC. Here, we further investigate potential role HPVs NMSC using a...
Several studies have suggested the involvement of cutaneous human papillomaviruses (HPVs) in development nonmelanoma skin cancers. Here we characterized vitro properties E7 proteins three HPV types, 10, 20, and 38, which are frequently detected specimens. We show that HPV38 is able to inactivate tumor suppressor pRb induces loss G(1)/S transition control, a key event carcinogenesis. In contrast, HPV10 HPV20 do not display these transforming activities. also two early E6 sufficient corrupt...
Establishment of a chronic infection is key event in virus-mediated carcinogenesis. Several cancer-associated, double-stranded DNA (dsDNA) viruses act via their oncoproteins to downregulate Toll-like receptor 9 (TLR9), the host innate immune response that senses viral or bacterial dsDNA. A novel oncogenic virus, Merkel cell polyomavirus (MCPyV), has been recently identified causes up 80% carcinomas (MCCs). However, it not yet known whether this virus also disrupts immune-related pathways. We...
ABSTRACT Innate immunity is the first line of host defense against infections. Many oncogenic viruses can deregulate several immune-related pathways to guarantee persistence infection. Here, we show that cutaneous human papillomavirus 38 (HPV38) E6 and E7 oncoproteins suppress expression double-stranded DNA sensor Toll-like receptor 9 (TLR9) in foreskin keratinocytes (HFK), a key mediator antiviral innate immune response. In particular, HPV38 induces TLR9 mRNA downregulation by promoting...
Head and neck squamous cell carcinomas (HNSCCs) represent a heterogeneous group of cancers for which human papilloma virus (HPV) infection is an emerging risk factor. Previous studies showed promoter hypermethylation in HPV(+) oropharyngeal cancers, but only few consistent target genes have been so far described, the evidence functional impact on gene expression still limited. We performed global stratified pooled analyses epigenome-wide data HNSCCs based Illumina HumanMethylation450...
The cutaneous beta human papillomavirus (beta HPV) types appear to be involved in skin carcinogenesis. However, only a few HPVs have been investigated so far. Here, we compared the properties of E6 and E7 oncoproteins from six uncharacterized (14, 22, 23, 24, 36, 49). Only HPV49 immortalized primary keratinocytes efficiently deregulated p53 pRb pathways. Furthermore, E6, similarly oncogenic HPV16, promoted degradation.
ABSTRACT Previous studies have shown that the PDZ-binding motif of E6 oncoprotein from mucosal high-risk (HR) human papillomavirus (HPV) types plays a key role in HPV-mediated cellular transformation vitro and vivo experimental models. HR HPV oncoproteins ability to efficiently degrade members PDZ motif-containing membrane-associated guanylate kinase (MAGUK) family; however, it is possible other proteins are also targeted by E6. Here, we describe novel interaction type 16 (HPV16) with...
Epstein–Barr Virus (EBV) infects more than 90% of the adult population worldwide. EBV infection is associated with Burkitt lymphoma (BL) though alone not sufficient to induce carcinogenesis implying involvement co-factors. BL endemic in African regions faced mycotoxins exposure. Exposure and oncogenic viruses has been shown increase cancer risks partly through deregulation immune response. A recent transcriptome profiling B cells exposed aflatoxin B1 (AFB1) revealed an upregulation Chemokine...
Abstract Human papillomavirus‐induced cervical carcinomas often show impaired expression of MHC class I molecules resulting in the inability tumor cells to directly present viral peptides cytotoxic T lymphocytes. Loss combined with activating NK cell receptor ligands renders potentially susceptible attack. Thus, this study, we analyzed ligands, accumulation and activation status situ normal ectocervical tissue (NCT), intraepithelial neoplasia (CIN) squamous carcinoma (CxCa). We observed that...
The oncoproteins E6 and E7 of human papillomavirus type 38 (HPV38) display several transforming activities in vitro, including immortalization primary keratinocytes. To evaluate the oncogenic viral proteins an vivo model, we generated transgenic mice expressing HPV38 under control bovine homologue keratin 10 (K10) promoter. Two distinct lines E6/E7-expressing that express genes at different levels were obtained. In both lines, induced cellular proliferation, hyperplasia, dysplasia epidermis....
The skin human papillomavirus (HPV) types belonging to the genus beta of HPV phylogenetic tree appear be associated with nonmelanoma cancer. We previously showed that type 38 E6 and E7 oncoproteins are able inactivate tumor suppressors p53 retinoblastoma. Here, both viral proteins were expressed in primary keratinocytes order study their effects on telomere/telomerase system. show immortalization induced by HPV38 E6/E7 is hTERT gene overexpression. This event is, part, explained accumulation...
The expression of the human papillomavirus (HPV) E6/E7 oncogenes is crucial for HPV-induced malignant cell transformation. identification cellular targets attacked by HPV critical our understanding molecular mechanisms HPV-associated carcinogenesis and may open novel therapeutic opportunities. Here, we identify Lens Epithelial-Derived Growth Factor (LEDGF) gene as a target oncogenes. Elevated LEDGF has been recently linked to can protect tumor cells towards different forms stress. We show...
Abstract The beta genus of human papillomaviruses (ß-HPV) includes approximately 50 different viral types that are subdivided into five species (ß-1 through ß-5). Nonmelanoma cancers may involve some ß-1 and ß-2 HPV types, but the biology most ß-HPV their possible connections to disease still little characterized. In this study, we studied effects ß-3 type HPV49 in a novel transgenic (Tg) mouse model, using cytokeratin K14 promoter drive expression E6 E7 genes from virus basal skin epidermis...
Interferon (IFN)-β inhibits cell proliferation and affects cycle in keratinocytes transformed by both mucosal high risk Human Papilloma Virus (HPV) cutaneous HPV E6 E7 proteins. In particular, upon longer IFN-β treatments, HPV38 expressing cells undergo senescence. appears to induce senescence upregulating the expression of tumor suppressor PML, a well known IFN-induced gene. Indeed, experiments gene silencing via specific siRNAs have shown that PML is essential execution programme p53 p21...
The DOK1 gene is a putative tumour suppressor located on the human chromosome 2p13 which frequently rearranged in leukaemia and other tumours. We previously reported that can be mutated its expression down-regulated malignancies. However, mechanism underlying silencing remains largely unknown. show here unscheduled of through aberrant hypermethylation frequent event variety was found to silenced nine head neck cancer (HNC) cell lines studied CpG correlated with loss these cells. could...
Epstein-Barr virus (EBV) was identified as the first human to be associated with a malignancy, Burkitt's lymphoma (BL), pediatric cancer endemic in sub-Saharan Africa. The exact mechanism of how EBV contributes process lymphomagenesis is not fully understood. Recent studies have highlighted genetic difference between (EBV+) and sporadic (EBV-) BL, variant showing lower somatic mutation load, which suggests involvement an alternative virally-driven transformation pathogenesis BL. We tested...
The histone modifier lysine (K)-specific demethylase 2B (KDM2B) plays a role in the differentiation of hematopoietic cells, and its expression appears to be deregulated certain cancers hematological lymphoid origins. We have previously found that KDM2B gene is differentially methylated cell lines derived from Epstein-Barr virus (EBV)-associated endemic Burkitt lymphoma (eBL) compared with EBV-negative sporadic lymphoma-derived cells. However, whether eBL development has not been...