A5040913442- interferon and immune responses
- HIV Research and Treatment
- Cytokine Signaling Pathways and Interactions
- Cervical Cancer and HPV Research
- Virus-based gene therapy research
- Immune Cell Function and Interaction
- Retinoids in leukemia and cellular processes
- Immunotherapy and Immune Responses
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Hepatitis C virus research
- Herpesvirus Infections and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- Erythrocyte Function and Pathophysiology
- NF-κB Signaling Pathways
- T-cell and B-cell Immunology
- Circular RNAs in diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer-related molecular mechanisms research
- Sarcoma Diagnosis and Treatment
- Iron Metabolism and Disorders
- Virology and Viral Diseases
- Nonmelanoma Skin Cancer Studies
- Genital Health and Disease
Institute of Molecular Biology and Pathology
2008-2023
Istituto Superiore di Sanità
2010-2023
San Gallicano Hospital
2019
Istituti di Ricovero e Cura a Carattere Scientifico
2019
Centre International de Recherche sur le Cancer
2019
Sapienza University of Rome
2008-2012
National Academies of Sciences, Engineering, and Medicine
2007
National Research Council
2003-2005
Istituto per le Tecnologie Didattiche
1990-1998
Institute of Biomedical Technologies
1990-1998
Abstract It has been recently reported that the endogenous expression of HIV-1 Nef in human monocyte/macrophages induces release chemokines and other as yet unidentified soluble factors leading to multiple effects pathogenic significance, such recruitment activation quiescent lymphocytes. However, description underlying molecular mechanisms remained elusive. We demonstrated monocyte-derived macrophages (MDM) efficiently internalize rNef, thereby inducing largely resembling those observed...
The viral protein Nef is a virulence factor that plays multiple roles during the early and late phases of human immunodeficiency virus (HIV) replication. regulates cell surface expression critical proteins (including down-regulation CD4 major histocompatibility complex class I), T-cell receptor signaling, apoptosis, inducing proapoptotic effects in uninfected bystander cells antiapoptotic infected cells. It has been proposed intersects CD40 ligand signaling pathway macrophages, leading to...
Background HIV-1 Nef is a virulence factor that plays multiple roles during HIV replication. Recently, it has been described intersects the CD40 signalling in macrophages, leading to modification pattern of secreted factors appear able recruit, activate and render T lymphocytes susceptible infection. The engagement by CD40L induces activation different cascades require recruitment specific tumor necrosis receptor-associated (i.e. TRAFs). We hypothesized TRAFs might be involved rapid NF-κB,...
Increasing evidence indicates that the expression of human immunodeficiency virus-1 (HIV-1) Nef protein significantly influences activation state host cell. Here we report specifically activates STAT3 in primary monocyte-derived macrophages (MDM). This was demonstrated by both single-cycle infection experiments driven Vesicular Stomatitis virus glycoprotein (VSV-G) pseudotyped HIV-1 and treatment with exogenous recombinant Nef. The analysis effects mutants revealed domains C-terminal...
The mechanisms that regulate the expression of H chain iron storage protein ferritin in Friend erythroleukemia cells (FLCs) after exposure to hemin (ferric protoporphyrin IX), IX, and ferric ammonium citrate (FAC) have been investigated. Administration increases steady-state level mRNA about 10-fold 20-fold. Experiments with transcriptional inhibitor actinomycin D transfection studies demonstrate increment cytoplasmic content results from enhanced transcription H-chain gene cannot be...
Interferon (IFN)-β inhibits cell proliferation and affects cycle in keratinocytes transformed by both mucosal high risk Human Papilloma Virus (HPV) cutaneous HPV E6 E7 proteins. In particular, upon longer IFN-β treatments, HPV38 expressing cells undergo senescence. appears to induce senescence upregulating the expression of tumor suppressor PML, a well known IFN-induced gene. Indeed, experiments gene silencing via specific siRNAs have shown that PML is essential execution programme p53 p21...
Actinic keratosis (AK) arises on photo-damaged skin and is considered to be the precursor lesion of cutaneous squamous cell carcinoma (cSCC). Many findings support involvement β human papillomaviruses (HPVs) in cSCC, while very little known γ HPV types. The objective this study was characterize spectrum PV types healthy (HS) AK samples same immunocompetent individuals using next generation sequencing (NGS). Viral DNA 244 242 HS specimens were amplified by PCR two different sets primers...
Background The potential role of the human immunodeficiency virus-1 (HIV-1) accessory protein Nef in pathogenesis neuroAIDS is still poorly understood. a molecular adapter that influences several cellular signal transduction events and membrane trafficking. In macrophages, expression induces production extracellular factors (e.g. pro-inflammatory chemokines cytokines) recruitment T cells, thus favoring their infection its own transfer to uninfected cells via exosomes, protrusions or...
Cells-FLC