A5044855008- Cancer Research and Treatments
- Virus-based gene therapy research
- Cervical Cancer and HPV Research
- RNA modifications and cancer
- interferon and immune responses
- Cytokine Signaling Pathways and Interactions
- Head and Neck Cancer Studies
- Nonmelanoma Skin Cancer Studies
- Immunotherapy and Immune Responses
- Cancer-related Molecular Pathways
- NF-κB Signaling Pathways
- Skin Protection and Aging
- Oral Health Pathology and Treatment
- Lymphoma Diagnosis and Treatment
- Drug Transport and Resistance Mechanisms
- Malaria Research and Control
- Monoclonal and Polyclonal Antibodies Research
- Genital Health and Disease
- Photodynamic Therapy Research Studies
- vaccines and immunoinformatics approaches
- Inflammasome and immune disorders
- Cancer therapeutics and mechanisms
- Respiratory viral infections research
- Ovarian function and disorders
- HIV/AIDS drug development and treatment
German Cancer Research Center
2012-2023
Heidelberg University
2011-2023
DKFZ-ZMBH Alliance
2011-2021
University of Turin
2005-2009
Zero to Three
2005
Multidrug resistance (MDR) is a phenomenon by which cancer cells evade the cytotoxic effects of chemotherapeutic agents. It may occur through different mechanisms, but it often correlates with overexpression integral membrane transporters, such as P-glycoprotein (Pgp) and MDR-associated proteins (MRPs), resulting decrease drug accumulation cellular death. Doxorubicin substrate Pgp; has been suggested that its ability to induce synthesis nitric oxide (NO) could explain, at least in part,...
Cutaneous beta human papillomavirus (HPV) types appear to be involved in the development of non-melanoma skin cancer (NMSC); however, it is not entirely clear whether they play a direct role. We have previously shown that E6 and E7 oncoproteins from HPV type 38 display transforming activities several experimental models. To evaluate possible contribution HPV38 proliferative tissue compartment during carcinogenesis, we generated new transgenic mouse model (Tg) where are expressed...
Cutaneous beta human papillomavirus (HPV) types are suspected to be involved, together with ultraviolet (UV) radiation, in the development of non-melanoma skin cancer (NMSC). Studies vitro and vivo experimental models have highlighted transforming properties HPV E6 E7 oncoproteins. However, epidemiological findings indicate that may required only at an initial stage carcinogenesis, become dispensable after full establishment NMSC. Here, we further investigate potential role HPVs NMSC using a...
Artemisinin is an antimalarial drug exerting pleiotropic effects, such as the inhibition of transcription factor nuclear factor-kappa B and sarcoplasmic/endoplasmic reticulum Ca(++)-ATPase (SERCA) P. falciparum. As sesquiterpene lactone thapsigargin, a known inhibitor mammalian SERCA, enhances expression P-glycoprotein (Pgp) by increasing intracellular Ca(++) ([Ca(++)](i)) level, we investigated whether artemisinin its structural homologue parthenolide could inhibit SERCA in human colon...
Abstract Multidrug resistant (MDR) tumor cells exhibit an altered pH gradient across different cell compartments, which favors a reduced intracellular accumulation of antineoplastic drugs and decreased therapeutic effect. In our study, we have observed that the activity expression Na + /H exchanger (NHE), is involved in homeostasis (pH i ), are increased doxorubicin‐resistant (HT29‐dx) human colon carcinoma comparison with doxorubicin‐sensitive HT29 cells. The was significantly higher...
Abstract The beta genus of human papillomaviruses (ß-HPV) includes approximately 50 different viral types that are subdivided into five species (ß-1 through ß-5). Nonmelanoma cancers may involve some ß-1 and ß-2 HPV types, but the biology most ß-HPV their possible connections to disease still little characterized. In this study, we studied effects ß-3 type HPV49 in a novel transgenic (Tg) mouse model, using cytokeratin K14 promoter drive expression E6 E7 genes from virus basal skin epidermis...
The lack of detectable precancerous lesions poses challenges to the early detection human papillomavirus-driven oropharyngeal cancer (HPV-OPC). Antibodies against HPV16 proteins, especially E6, are uniquely sensitive and specific biomarkers years prior HPV-OPC diagnosis. Thus, protein serology warrants clinical investigation for screening.Using multiplex serology, we analyzed serum antibodies first 5000 participants (n=4,424 sera, recruited 2016-2017) Hamburg City Health Study, a...
Many findings support a possible involvement of subgroup human papillomaviruses (HPVs), called cutaneous beta HPV types, in the development non-melanoma skin cancer. The transgenic (Tg) mice expressing viral oncoproteins E6 and E7 from different including HPV38, showed an increased susceptibility to UV-induced and/or chemically induced carcinogenesis compared with wild-type animals. In this study, we show that HPV38 act as promoter progression factors multi-stage carcinogenesis, strongly...
The beta human papillomaviruses (HPVs) are subdivided into 5 species (beta-1 to beta-5), and they were first identified in the skin. However, beta-3 appears be more highly represented mucosal epithelia than Functional studies have also highlighted that HPV49 shares some functional similarities with high-risk (HR) HPV16. Here, we describe characterization of
Tumor suppressors can exert pro-proliferation functions in specific contexts. In the beta human papillomavirus type 38 (HPV38) experimental model, viral proteins E6 and E7 promote accumulation of a wild-type (WT) p53 form keratinocytes (HKs), promoting cellular proliferation. Inactivation by different means strongly decreases proliferation HPV38 E6/E7 HKs. This is phosphorylated at S392 double-stranded RNA-dependent protein kinase PKR, which highly activated HPV38. PKR-mediated...
<div>Abstract<p>The beta genus of human papillomaviruses (ß-HPV) includes approximately 50 different viral types that are subdivided into five species (ß-1 through ß-5). Nonmelanoma cancers may involve some ß-1 and ß-2 HPV types, but the biology most ß-HPV their possible connections to disease still little characterized. In this study, we studied effects ß-3 type HPV49 in a novel transgenic (Tg) mouse model, using cytokeratin K14 promoter drive expression E6 E7 genes from virus...
<p>Tumor burden in WT and HPV E6/E7-Tg mouse lines of type 38 line 187 49 2 after DMBA/TPA treatment</p>
<p>Legends of supplementary Figures 1-3</p>
<p>Additional information of methods used in the study</p>
<p>E6/E7 gene copy number and E6/E7 transcript levels in the different HPV Tg animal models.</p>