A5080683449- Reproductive Biology and Fertility
- Reproductive Health and Technologies
- Ovarian function and disorders
- Assisted Reproductive Technology and Twin Pregnancy
- Sperm and Testicular Function
- Prenatal Screening and Diagnostics
- Effects and risks of endocrine disrupting chemicals
- Animal Genetics and Reproduction
- Renal and related cancers
- Apelin-related biomedical research
- Pluripotent Stem Cells Research
- Adipokines, Inflammation, and Metabolic Diseases
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Molecular Biology Techniques and Applications
- Reproductive System and Pregnancy
- Adipose Tissue and Metabolism
- Birth, Development, and Health
- Lipid metabolism and disorders
- Cell death mechanisms and regulation
- Kruppel-like factors research
- Omental and Epiploic Conditions
- MicroRNA in disease regulation
- Antioxidant Activity and Oxidative Stress
- RNA Interference and Gene Delivery
- Advanced Biosensing Techniques and Applications
Physiologie de la Reproduction et des Comportements
2016-2025
Centre Hospitalier Universitaire de Tours
2013-2025
Université de Tours
2013-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2025
Centre National de la Recherche Scientifique
2009-2024
Hôpital Bretonneau
2004-2024
Centre Val de Loire
2006-2022
Institut Français
2019
Biologie de la Reproduction, Environnement, Epigénétique et Développement
2008-2017
Hull York Medical School
2012-2013
Dialogue between the oocyte and cumulus cells is essential for maturation. A prospective laboratory research project was designed to evaluate transcription of specific genes in harvested before intracytoplasmic sperm injection from pre-ovulatory follicles, according individual nuclear maturity developmental competence. Genes were chosen because their expression induced by LH peak [Steroidogenic Acute Regulatory protein ( STAR ), Cyclooxygenase 2 COX2 or PTGS2 Amphiregulin AREG )] they...
Non-invasive and routine developmental markers are available to select the most viable embryo; however their respective values in terms of blastocyst development potential remain difficult distinguish.During this prospective study, sequential growth 4042 embryos individually cultured from day 1 5/6 was recorded. Pronuclear morphology on 1, early cleavage, cell number fragmentation rate 2 were evaluated for each zygote. Additionally, transfers analysed with regard implantation ability embryo...
Chemerin is a novel adipokine involved in the regulation of adipocyte development, inflammation and metabolic functions. To date, no role this reproductive functions has been described. In present study, we identified chemerin its receptor, CMKLR1 (chemokine-like receptor 1), primary human granulosa cells (hGCs) ovarian granulosa-like tumour cell line (KGN). We also investigated effects recombinant (rhChem) on steroid production various signalling pathways. By RT–PCR immunoblotting...
Apelin (APLN) is a recently discovered adipokine involved in the regulation of various metabolic functions. Its receptor, APLNR, expressed reproductive tissues, however, its role human ovarian cells unknown. In this study, we identified APLN and APLNR follicles analyzed their expression granulosa follicular fluid obtained from obese nonobese patients, with or without polycystic ovary syndrome (PCOS). We also investigated effect on steroidogenesis cultured luteinized (hGCs) patients PCOS....
Visfatin is a cytokine hormone and an enzyme involved in metabolic (obesity, type II diabetes) immune disorders. Some data suggest role of visfatin ovarian function. Here, we identified human follicles investigated the molecular mechanisms regulation its expression response to insulin sensitizers, metformin (MetF) rosiglitazone, primary granulosa cells (hGCs) granulosa-like tumour cell line (KGN). We also studied effects recombinant (RhVisf) on steroid production activation various...
Background Single embryo transfer (SET) is the most successful way to reduce frequency of multiple pregnancies following in vitro fertilisation. However, selecting for SET with highest chances pregnancy remains a difficult challenge since morphological and kinetics criteria provide poor prediction both developmental implantation ability. Partly through expression specific genes, oocyte-cumulus interaction helps oocyte acquire its competence. Our aim was therefore identify at level cumulus...
Adipokines are a potential link between reproduction and energy metabolism could partly explain some infertilities related to pathophysiology, such as polycystic ovary syndrome (PCOS). However, adipokines were predominantly assessed in blood samples, while very little is known concerning their variations follicular fluid (FF) ovarian granulosa cells (GCs) of PCOS women. Thus, the objectives our study investigate adiponectin, chemerin, resistin, visfatin, omentin, apelin expression women...
The purpose of this study was to evaluate the respective influences blastomere survival and resumption mitosis on outcome frozen-thawed embryos.A retrospective analysis performed in our centre 363 thawing cycles, involving 4-cell day 2 grade 1 embryos with <10% fragmentation.A higher implantation rate per transferred embryo observed when all were characterized by fully intact blastomeres (100% survival) as compared damaged (50 or 75% (22.0 versus 7.2%; P < 0.0001). Moreover, significantly...
Non-invasive assay of the consumption and release metabolites by individual human embryos could allow selection at cleavage stage development facilitate Single Embryo Transfer in clinical IVF but will require simple, high throughput, sensitive methods applicable to small volume samples. A rapid, non-invasive method has therefore been devised using a standard fluorescence plate reader, used measure pyruvate glucose, lactate single bovine all stages preimplantation culture; amino acid profiles...
Bisphenol S (BPS) is a structural analog of the endocrine disruptor bisphenol A (BPA); it main BPA replacement in plastics industry. Previous studies have shown that and BPS exhibit similar effects on reproduction fish rodent species. reportedly alters steroidogenesis bovine granulosa cells. Luteinised cells collected from 59 women who were undergoing an vitro fertilization procedure cultured for 48 h presence or absence (10 nM, 100 1 µM, 10 µM 50 µM). exposure was investigated by assessing...
Abstract Background According to current definitions of Polycystic Ovary Syndrome (PCOS), hyperandrogenism is considered as a key element in the pathogenesis this common endocrinopathy. However, until now, studies about ovarian androgen profile women are very rare. Our aim was then characterise expression androgens follicular fluid 30 PCOS patients, and compare it those 47 Control 29 with only polycystic ovary morphology on ultrasounds (ECHO group). Methods A retrospective, single-centre...
Bisphenol (BP) structural analogues of BPA are widely used. Previous studies showed similar effects and BPS on reproduction in several species including human. We hypothesised that the bisphenols (BPs) could accumulate granulosa cells (GCs) affects steroidogenesis. This study investigated seven BP their equimolar cocktail human (hGC) assessed BPA, BPS, BPF BPAF level exposures follicular fluid 277 women undergoing Assisted Reproductive Technology. The hGCs were recovered after oocyte...
Whether extended culture allowing selection of embryos with high development potential has any advantage over cleavage-stage embryo transfer remains a matter debate. Among the currently unsolved questions, cumulative delivery rate resulting from fresh and frozen transfers needs to be taken into account in both strategies. The aim our study was, therefore, compare efficacy single either on Day 2 or 5/6 combining transfers.A prospective including 478 couples assigned voluntary basis undergo...
Although it has been suspected that there is a decrease in semen quality over time, the results reported to date remain debatable because of methodological issues. The aim study here was investigate evolution time population 1114 fertile candidates for sperm donation at CECOS, Tours, between 1976 and 2009. We investigated volume, concentration, progressive motility, vitality, percentage normal forms multiple abnormalities index first ejaculate this population. did not find decline whereas we...
INTELECTIN (ITLN) is an adipokine involved in the regulation of insulin sensitivity and inflammatory immunity responses. Serum ITLN levels are lower obese, diabetic, polycystic ovary syndrome (PCOS) women than control subjects. has never been studied ovarian cells. Here, we identified ITLN1 human follicles investigated molecular mechanisms its expression response to sensitizers metformin rosiglitazone, granulosa-lutein cells (hGLCs) a granulosa-like tumor cell line (KGN). We also effects...
Reactive oxygen species (ROS) which lead to oxidative stress affect ovarian function. Grape seed extract (GSE) could be proposed as an effective antioxidant, particularly due its proanthocyanidin content. In this study, we investigated a dose effect (0, 0.01, 0.1, 1, 10, 50, and 100 μg/mL) of GSE B2 (GSPB2) on the ROS content, cell proliferation, viability, steroidogenesis in both primary luteinized granulosa cells (hGC) tumor line (KGN). The levels were measured using ROS-Glo assay. Cell...
Abstract STUDY QUESTION What biological processes are linked to the signaling of energy sensor 5′-AMP-activated protein kinase (AMPK) in mouse and human granulosa cells (GCs)? SUMMARY ANSWER The lack α1AMPK GCs impacted cell cycle, adhesion, lipid metabolism induced a hyperandrogenic response. WHAT IS KNOWN ALREADY AMPK is expressed ovarian follicle, its activation by pharmacological medications, such as metformin, inhibits production steroids. Polycystic ovary syndrome (PCOS) responsible...
Abstract Context Bisphenol A (BPA) and its analogues disrupt endocrine functions, adversely impacting oocyte meiosis, maturation, granulosa cell (GC) steroidogenesis. Objective To identify clinical factors, particularly adiposity age, influencing ovarian sensitivity to bisphenol (BP) exposure. Methods This study analysed a cohort of 368 women undergoing assisted reproductive technology (ART) from 2019 2023. Four BPs (BPA, BPS, BPF, BPAF) were quantified, ART outcomes (e.g., count, embryo...
During spermiogenesis, the proximal centriole forms a special microtubular structure: centriolar adjunct. This structure appears at spermatid stage, which is characterized by condensed chromatin nucleus. We showed that adjunct disappears completely in mature porcine spermatozoa. In humans, remnants are present fraction of spermatids. For first time, cell, and its consequent impact on fertility, were examined. Ultrastructural analysis using transmission electron microscopy was performed near...