A5060675130- Effects and risks of endocrine disrupting chemicals
- Reproductive Biology and Fertility
- Fatty Acid Research and Health
- Reproductive Physiology in Livestock
- Genetic and phenotypic traits in livestock
- Birth, Development, and Health
- Regulation of Appetite and Obesity
- Metabolomics and Mass Spectrometry Studies
- Adipose Tissue and Metabolism
- Ruminant Nutrition and Digestive Physiology
- Lipid metabolism and biosynthesis
- Ovarian function and disorders
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Insect Utilization and Effects
- Effects of Environmental Stressors on Livestock
- Soil erosion and sediment transport
- Meat and Animal Product Quality
- Sperm and Testicular Function
- Molecular Biology Techniques and Applications
- Nutrition, Genetics, and Disease
- Adipokines, Inflammation, and Metabolic Diseases
- Muscle metabolism and nutrition
- Animal Genetics and Reproduction
- Natural Products and Biological Research
- Toxic Organic Pollutants Impact
Physiologie de la Reproduction et des Comportements
2015-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Université de Tours
2013-2024
Centre National de la Recherche Scientifique
2013-2024
Oocyte meiotic maturation requires energy from various substrates including glucose, amino acids, and lipids. Mitochondrial fatty acid (FA) β-oxidation (FAO) in the oocyte is required for maturation, which accompanied by differential expression of numerous genes involved FAs metabolism surrounding cumulus cells (CCs) vivo. The objective was to elucidate components CCs during maturation. Twenty-seven related lipogenesis, lipolysis, FA transport, FAO were chosen comparative transcriptome...
Ovarian granulosa cells (GC) are essential for the development and maturation of a proper oocyte. GC sensitive to endocrine disruptors, including bisphenol A (BPA) its analogue S (BPS), plasticisers present in everyday consumer products. BPA exhibits greater binding affinity membrane oestrogen receptor (GPER) than nuclear receptors (ERα ERβ). Here, we analysed effects BPS on steroidogenesis ovine vitro, as well their early mechanisms action, being relevant model study human reproductive...
Bisphenol S (BPS) is a structural analog of the endocrine disruptor bisphenol A (BPA); it main BPA replacement in plastics industry. Previous studies have shown that and BPS exhibit similar effects on reproduction fish rodent species. reportedly alters steroidogenesis bovine granulosa cells. Luteinised cells collected from 59 women who were undergoing an vitro fertilization procedure cultured for 48 h presence or absence (10 nM, 100 1 µM, 10 µM 50 µM). exposure was investigated by assessing...
Bisphenol (BP) structural analogues of BPA are widely used. Previous studies showed similar effects and BPS on reproduction in several species including human. We hypothesised that the bisphenols (BPs) could accumulate granulosa cells (GCs) affects steroidogenesis. This study investigated seven BP their equimolar cocktail human (hGC) assessed BPA, BPS, BPF BPAF level exposures follicular fluid 277 women undergoing Assisted Reproductive Technology. The hGCs were recovered after oocyte...
In mammals, oocytes develop inside the ovarian follicles; this process is strongly supported by surrounding follicular environment consisting of cumulus, granulosa and theca cells, fluid. antral follicle, final stages oogenesis require large amounts energy that produced cells from substrates including glucose, amino acids fatty (FAs). Since lipid metabolism plays an important role in acquiring oocyte developmental competence, aim study was to investigate site-specificity ovaries comparing...
Bisphenols, plasticisers used in food containers, can transfer to food. Bisphenol A (BPA) has been described as an endocrine disruptor and consequently banned from the industry several countries. It was replaced by a structural analogue, S (BPS). BPA action on steroidogenesis is one of mechanisms underlying its adverse effects efficiency female reproduction. This study aimed determine whether BPS safe alternative regarding GC functions. Antral follicles (2–6 mm), approximatively 1000 adult...
Docosahexaenoic acid (DHA) is a n-3 polyunsaturated fatty (PUFA) belonging to family of biologically active acids (FA), which are known have numerous health benefits. N-3 PUFAs affect reproduction in cattle, and notably directly follicular cells. In terms PUFA-enriched diets lead increased follicle size or numbers. The objective the present study was analyze effects DHA (1, 10, 20 50 μM) on proliferation steroidogenesis (parametric and/or non parametric (permutational) ANOVA) bovine...
Introduction: Bisphenol A (BPA) is a widespread compound in the plastic industry that especially used to produce baby bottles, food packaging and metal cans. BPA, an endocrine disruptor, leads alterations reproductive function therefore has been banned from industry. Unregulated BPA analogues, particularly S (BPS), have emerged are now Thus, this study aimed examine acute effects of low environmental doses BPS on ewe oocyte quality developmental competence, its mechanism action, during vitro...
Bisphenol A (BPA), a plasticizer and endocrine disruptor, has been substituted by bisphenol S (BPS), structural analogue that had already shown adverse effects on granulosa cell steroidogenesis. The objective of this study was to assess the effect chronic exposure BPS, possible steroid hormones in ovary, oviduct plasma using ewe as model. Given interaction between steroidogenesis metabolic status, BPS tested according two diet groups. Eighty adult ewes were allotted restricted (R) well-fed...
In mammals, the Tribbles family includes widely expressed serine-threonine kinase-like proteins (TRIB1, TRIB2 and TRIB3) that are involved in multiple biological processes including cell proliferation fatty acid (FA) metabolism. Our recent studies highlighted importance of FA metabolism cumulus cells (CC) during oocyte maturation vertebrates reported a higher TRIB1 expression CC surrounding vivo mature oocytes as compared to immature ooocytes mice cows. The objective was investigate patterns...
Visfatin and resistin appear to interfere with reproduction in the gonads, but their potential action at hypothalamic-pituitary level is not yet known. The aim of present study was investigate mRNA protein expression these adipokines murine gonadotroph cells analyse effects different concentrations recombinant mouse visfatin (0.01, 0.1, 1 10ngmL-1) on LH secretion signalling pathways LβT2 and/or primary female pituitary cells. Both were found vivo In contrast resistin, tissue source skeletal...
Bisphenol A (BPA), an endocrine disruptor, has been replaced by structural analogues including bisphenol S (BPS). BPA and BPS exhibited similar effects regarding reproductive functions. Moreover, metabolic status lipid metabolism are related to female fertility could worsen effects. The objective was determine in vivo on folliculogenesis embryo production after chronic exposure through diet, the influence of adult ewes. Sixty primiparous 2.5 year-old ewes, undergoing a restricted or well fed...
Beneficial effects of n-3 polyunsaturated fatty acid (PUFA) supplementation on dairy cow reproduction have been previously reported. The objectives the present study were to assess whether PUFA would affect in vitro embryo production (IVP) after ovarian stimulation. Holstein cows received a diet with 1% dry matter either (n = 18, microencapsulated fish oil) or control, n-6 19, soy oil). Both plasma and follicular fluid FA composition showed integration total through diet. All underwent an...
Do plastic laboratory consumables and cell culture media used in ART contain bisphenols?The majority of human embryo assessed contained bisphenol S close to the nanomolar concentration range, while no release bisphenols by was detected under routine conditions.The deleterious effect endocrine disruptor A (BPA) on female fertility raised concerns regarding outcome. BPA neither nor ART; however, it might have already been replaced its structural analogs, including (BPS).Seventeen 18 were for...
Bisphenol S (BPS) affects terminal folliculogenesis by impairing steroidogenesis in granulosa cells from different species. Nevertheless, limited data are available on its effects during basal folliculogenesis. In this study, we evaluate vitro the of a long-term BPS exposure model follicular development mono-ovulatory We cultured ovine preantral follicles (180−240 μm, n = 168) with (0.1 μM (possible human dose) or 10 (high dose)) and monitored antrum appearance survival growth for 15 days....
This work presents a systematic approach to study the conservation of genes between fruit flies and mammals. We have listed 971 Drosophila involved in female reproduction at ovarian level systematically looked for orthologs Ciona, zebrafish, coelacanth, lizard, chicken, mouse. Depending on species, percentage these with least one ortholog varies 69% 78%. In comparison, only 42% all an mouse genome (P < 0.0001), suggesting dramatically higher evolutionary genes. The 177 that no mice other...
In mammals, the medio-basal hypothalamus (MBH) integrates photoperiodic and food-related cues to ensure timely phasing of physiological functions, including seasonal reproduction. The current human epidemics obesity associated reproductive disorders exemplifies tight link between metabolism Yet, how impact breeding at level MBH remains unclear. this respect, sheep, which is a large diurnal mammal with marked dual photoperiodic/metabolic control breeding, relevant model. Here, we present...