A5027757155- Diabetes Treatment and Management
- Metabolism, Diabetes, and Cancer
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Diabetes Management and Research
- RNA and protein synthesis mechanisms
- Diabetes and associated disorders
- DNA and Nucleic Acid Chemistry
- Diabetes Management and Education
- Chronic Disease Management Strategies
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Pancreatic function and diabetes
- Liver Disease Diagnosis and Treatment
- Cellular transport and secretion
- Telomeres, Telomerase, and Senescence
- Heart Failure Treatment and Management
- Health Promotion and Cardiovascular Prevention
- Health disparities and outcomes
- Obesity, Physical Activity, Diet
- Cardiovascular Health and Risk Factors
- Receptor Mechanisms and Signaling
- Advanced biosensing and bioanalysis techniques
- Chronic Kidney Disease and Diabetes
- Neuroscience and Neuropharmacology Research
- Frailty in Older Adults
Chinese University of Hong Kong
2018-2025
Prince of Wales Hospital
2018-2025
Children's Hospital of Fudan University
2020-2025
Wenzhou Medical University
2024
Affiliated Eye Hospital of Wenzhou Medical College
2024
Chongqing University
2022-2023
University of Edinburgh
2016-2019
Glasgow Centre for Population Health
2019
University of Pennsylvania
2018
Ionis Pharmaceuticals (United States)
1998-2013
Catecholamine secretory vesicle core proteins (chromogranins) contain an activity that inhibits catecholamine release, but the identity of responsible peptide has been elusive. Size-fractionated chromogranins antagonized nicotinic cholinergic-stimulated secretion; inhibitor was enriched in processed chromogranin fragments, and liberated from purified A. Of 15 synthetic peptides spanning approximately 80% A, one (bovine A344-364 [RSMRLSFRARGYGFRGPGLQL], or catestatin) a potent, dose-dependent...
The secretory prohormone chromogranin A (CHGA) is overexpressed in essential hypertension, a complex trait with genetic predisposition, while its catecholamine release-inhibitory fragment catestatin diminished, and low predicts augmented adrenergic pressor responses. These findings from studies on humans suggest mechanism whereby diminished might increase the risk for hypertension. We generated Chga humanized mice through transgenic insertion of human CHGA haplotype order to probe vivo....
A human RNase III gene encodes a protein of 160 kDa with multiple domains, proline-rich, serine- and arginine-rich, an domain. The expressed purified domain cleaves double-strand RNA does not cleave single-strand RNA. is ubiquitously in tissues cell lines, the localized nucleus cell. levels transcription translation do change during different phases cycle. However, significant fraction translocated to nucleolus S phase That this involved processing pre-rRNA, but might at sites from those...
OBJECTIVE Several studies support associations between relative leukocyte telomere length (rLTL), a biomarker of biological aging and type 2 diabetes. This study investigates the relationship rLTL risk glycemic progression in patients with RESEARCH DESIGN AND METHODS In this cohort study, consecutive Chinese diabetes (N = 5,506) from Hong Kong Diabetes Register stored baseline DNA available follow-up data were studied. was measured using quantitative PCR. Glycemic defined as new need for...
Background Clinical trials have demonstrated that remission of type 2 diabetes can be achieved following sustained weight loss. However, the feasibility achieving through management in real-world settings remains unclear. In this study, we aimed to examine association change at 1 year after diagnosis with long-term incidence and sustainability Hong Kong. Methods findings This was a population-based observational cohort study. The territory-wide Risk Assessment Management Programme for...
BackgroundCurrent labelling advises discontinuation of metformin when estimated glomerular filtration rate (eGFR) < 30 ml/min/1.73 m2 due to increased risk lactic acidosis. However, in real-world practice, the risk-benefit ratios remain uncertain. We examined associations discontinued-metformin use with cardiorenal and clinical outcomes patients type 2 diabetes (T2D) advanced chronic kidney disease.MethodsIn this territory-wide, retrospective cohort target trial emulation study, we included...
We have characterized cloned His-tag human RNase H1. The activity of the enzyme exhibited a bell-shaped response to divalent cations and pH. optimum conditions for catalysis consisted 1 mm Mg2+ pH 7–8. In presence Mg2+, Mn2+ was inhibitory. Human H1 shares many enzymatic properties with Escherichia coliRNase cleaves RNA in DNA-RNA duplex resulting products 5′-phosphate 3′-hydroxy termini, can cleave overhanging single strand adjacent duplex, is unable substrates which either or DNA has 2′...
Abstract Background Vitamin D deficiency is highly prevalent across the globe. Existing studies suggest that a low vitamin level associated with more than 130 outcomes. Exploring causal role of in health outcomes could support or question supplementation. Methods We carried out systematic literature review previous Mendelian-randomization on D. then implemented Mendelian Randomization–Phenome Wide Association Study (MR-PheWAS) analysis data from 339 256 individuals White British origin UK...
There is very limited data on the time trend of diabetes incidence in Asia. Using population-level data, we report secular type 1 and 2 Hong Kong between 2002 2015.The Diabetes Surveillance Database hosts clinical information people with receiving care under Hospital Authority, a statutory body that governs all public hospitals clinics. Sex-specific rates were standardised to age structure World Health Organization population. Joinpoint regression analysis was used describe trends. A total...
Rationale & ObjectiveNonalbuminuric diabetic kidney disease (DKD) has become the prevailing DKD phenotype. We compared risks of adverse outcomes among patients with this phenotype other phenotypes.Study DesignMulticenter prospective cohort study.Settings Participants19,025 Chinese adults type 2 diabetes enrolled in Hong Kong Diabetes Biobank.ExposuresDKD phenotypes defined by baseline estimated glomerular filtration rate (eGFR) and albuminuria: no (no decreased eGFR or albuminuria),...
Background The prevalence of type 2 diabetes has increased in both young and old people. We examined age-specific associations population attributable fractions (PAFs) risk factors for all-cause cause-specific mortality people with diabetes. Methods findings analysed data from 360,202 Chinese who participated a territory-wide complication screening programme Hong Kong between January 2000 December 2019. compared the hazard ratios PAFs eight factors, including three major comorbidities...
The aim of this study was to describe the metabolome in diabetic kidney disease (DKD) and its association with incident CVD type 2 diabetes, identify prognostic biomarkers.
Chromogranin A is the index member of a family acidic proteins stored and released throughout neuroendocrine system with peptide hormones neurotransmitters. To better understand its functional domains, evolutionary lineage, basis tissue-specific pattern expression, we obtained mouse chromogranin cDNA used it to isolate chromogrannin gene from genome. We then characterized gene's exon/intron structure, structure function 5'-regulatory region (promoter/enhancer). The was complex, eight exons...
In a previous study, we demonstrated that the sugar conformation and helical geometry of heteroduplex substrate at catalytic site human RNase H1 directs selective recognition by enzyme (J Biol Chem 279: 36317-36326, 2004). this systematically introduced 2'-methoxyethoxy (MOE) nucleotides into antisense oligodeoxyribonucleotide (ASO) to alter substrate. The MOE substitutions 3' 5' poles ASO resulted in fewer cleavage sites slower rates compared with unmodified substrates. Furthermore, greater...
OBJECTIVE Several studies support potential links between relative leukocyte telomere length (rLTL), a biomarker of biological aging, and type 2 diabetes. This study investigates relationships rLTL incident cardiovascular disease (CVD) in patients with RESEARCH DESIGN AND METHODS Consecutive Chinese diabetes (N = 5,349) from the Hong Kong Diabetes Register for whom DNA obtained at baseline was stored follow-up data were available studied. measured by using quantitative PCR. CVD diagnosed on...
OBJECTIVE There has been a shift toward new classes of glucose-lowering drugs (GLDs) in the past decade but no improvements glycemic control or hospitalization rates due to severe hypoglycemia (SH) previous surveys. We examined trends GLDs use, control, and SH rate among patients with diabetes Hong Kong, which introduced territory-wide, team-based care model since 2000. RESEARCH DESIGN AND METHODS Using population-based data from Kong Diabetes Surveillance Database, we estimated age-...