A5076733570- CRISPR and Genetic Engineering
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Occupational and environmental lung diseases
- Cancer Cells and Metastasis
- Lung Cancer Diagnosis and Treatment
- Pluripotent Stem Cells Research
- Dietary Effects on Health
- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Chemical and Physical Properties of Materials
- Skin Protection and Aging
- RNA Interference and Gene Delivery
- Genetics, Aging, and Longevity in Model Organisms
- Single-cell and spatial transcriptomics
- nanoparticles nucleation surface interactions
- Digestive system and related health
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Molecular Biology Techniques and Applications
- Animal Genetics and Reproduction
- Viral Infectious Diseases and Gene Expression in Insects
- Animal Virus Infections Studies
- Advanced biosensing and bioanalysis techniques
- Renal and related cancers
- Radiation Dose and Imaging
University of Pennsylvania
2014-2022
Genentech
2014
West Virginia University
2008
Direct reprogramming by overexpression of defined transcription factors is a promising new method deriving useful but rare cell types from readily available ones. While the presents numerous advantages over induced pluripotent stem (iPS) approaches, focus on murine conversions and reliance retroviral vectors limit potential human applications. Here we address these concerns demonstrating direct conversion fibroblasts to hepatocyte-like cells via repeated transfection with synthetic modified...
Highlights•Calorie restriction increases the radioresistant intestinal stem cell (ISC) pool•Calorie suppresses mTORC1 activity in reserve ISCs•Reserve ISC-autonomous governs ISC survival post injury•Calorie-restricted ISCs have enhanced regenerative capacity injurySummaryAging is a complex process associated with decline functionality of adult cells affecting tissue homeostasis and regeneration. Calorie (CR) only experimental manipulation known to extend lifespan reduce incidence age-related...
The level of atmospheric oxygen, a driver free radical damage and tumorigenesis, decreases sharply with rising elevation. To understand whether ambient oxygen plays role in human carcinogenesis, we characterized age-adjusted cancer incidence (compiled by the National Cancer Institute from 2005 to 2009) across counties elevation-varying Western United States compared trends displayed respiratory (lung) non-respiratory cancers (breast, colorectal, prostate). adjust for important demographic...
Assessing mammalian gene function in vivo has traditionally relied on manipulation of the mouse genome embryonic stem cells or perizygotic embryos. These approaches are time-consuming and require extensive breeding when simultaneous mutations multiple genes is desired. The aim this study to introduce a rapid multiplexed editing (RIME) method provide proof concept system.
Abstract Metastatic cancer remains largely incurable due to an incomplete understanding of how cells disseminate throughout the body. However, tools for probing metastatic dissemination and associated molecular changes at high resolution are lacking. Here we present multiplexed, activatable, clonal, subclonal GESTALT (macsGESTALT), inducible lineage recorder with concurrent single cell readout transcriptional phylogenetic information. By integrating multiple copies combined static barcodes...
The atmospheric concentration of oxygen, a driver free radical damage and tumorigenesis, decreases sharply with rising elevation. To understand whether ambient oxygen concentrations play role in human carcinogenesis, we characterized age-adjusted cancer incidence (compiled by the National Cancer Institute from 2005–2009) across counties elevation-varying Western United States compared trends displayed respiratory (lung) non-respiratory cancers (breast, colorectal, prostate). adjust for...
Abstract Background & Aims Assessing mammalian gene function in vivo has traditionally relied on manipulation of the mouse genome embryonic stem cells or peri-zygotic embryos. These approaches are time consuming and require extensive breeding when simultaneous mutations multiple genes is desired. The aim this study to introduce a R apid I n M ultiplexed Editing (RIME), provide proof-of-concept system. Approach Results RIME, system wherein CRISPR/Cas9 technology, paired with...
The atmospheric concentration of oxygen, a driver free radical damage and tumorigenesis, decreases sharply with rising elevation. To understand whether ambient oxygen concentrations play role in human carcinogenesis, we characterized age-adjusted cancer incidence (compiled by the National Cancer Institute from 2005–2009) across counties elevation-varying Western United States compared trends displayed respiratory (lung) non-respiratory cancers (breast, colorectal, prostate). adjust for...
The atmospheric concentration of oxygen, a driver free radical damage and tumorigenesis, decreases sharply with rising elevation. To understand whether ambient oxygen concentrations play role in human carcinogenesis, we characterized age-adjusted cancer incidence (compiled by the National Cancer Institute from 2005–2009) across counties elevation-varying Western United States compared trends displayed respiratory (lung) non-respiratory cancers (breast, colorectal, prostate). adjust for...