Dengue disease is still a major health problem in Indonesia. Surabaya, the second largest city country, endemic for dengue. We report here on dengue investigating clinical manifestations, distribution of virus (DENV) serotypes, and relationships between manifestations genetic characteristics DENV. A total 148 patients suspected having were recruited during February-August 2012. One hundred one (68%) them children, 47 (32%) adults. fever (DF) hemorrhagic (DHF) equally manifested all patients....

Abstract Background Early diagnosis of dengue infection is crucial for better management the disease. Diagnostic tests based on detection virus (DENV) Non Structural Protein 1 (NS1) antigen are commercially available with different sensitivities and specificities observed in various settings. Dengue endemic Indonesia clinicians increasingly using NS1 confirmation. This study described performance Panbio IgM Capture ELISA assays during our surveillance eight cities as well genetic diversity...

Diagnostic tests based on detection of dengue virus (DENV) genome are available with varying sensitivities and specificities. The Simplexa Dengue assay (Focus Diagnostics) is a newly developed real-time RT-PCR method designed to detect serotype DENV simultaneously. To assess the performance assay, we performed comparison conventional SYBR Green patients sera isolated from eight cities across Indonesia, endemic country. A total 184 that were confirmed using NS1 and/or IgM IgG ELISA examined....

Introduction: Pseudomonas aeruginosa is a Gram-negative pathogen that causes major infections in patients with weak body defense mechanisms. The pelA gene encodes the Pel polysaccharide for production of cationic charged polysaccharides, and expression polysaccharide-encoding locus (pelA) by essential biofilm formation.Objective: Analyzing relationship between density clinical isolates aeruginosa.Method: Analytical observational research design consecutive sampling, total sample 33...

Introduction: One of the main causes hospitalization and death is NSTE-ACS. A GRACE risk score tool specifically designed for stratification acute coronary syndrome patients needed. The has limitations related to uncertainty atherosclerotic plaque rupture difficulty in predicting. GDF-15 one possibility that necessary because results depend on an assessment at a point time. Elevated circulating levels are associated with poor prognosis many chronic conditions including heart disease....

Introduction: Infection due to Pseudomonas aeruginosa is a cause of nosocomial infections that are acquired when patients hospitalized. The incidence bacterial 80% related biofilm formation, which the main mediator infection. genetically produces at least three polysaccharides help formation process and maintain stability structure, one algD gene. Objective: Analyze relationship between presence gene density in clinical isolates aeruginosa. Method: analytical observational research,...

Outbreaks of dengue virus (DENV) in Indonesia have been mainly caused by the DENV serotype-1; -2; or -3. The DENV-4 was least-reported serotype during last five decades. We recently conducted a molecular epidemiology study Jember regency, East Java province, Indonesia. Dengue is endemic region and outbreaks occur annually. investigated clinical characteristics etiology dengue-like febrile illness this regency to understand disease dynamics. A total 191 patients with symptoms similar were...

Background: The diagnostic test for malaria is mostly based on Rapid Diagnostic Test (RDT) and detection by microscopy. Polymerase Chain Reaction (PCR) also a sensitive method that can be considered as tool. outcome of microscopy depends the examiner's ability experience. Some RDT has been distributed in Indonesia, which needs to evaluated their results. Objective: This study aimed compare performance RightSign ScreenPlus Plasmodium human blood. We used specific real-time polymerase chain...

Background: Acute coronary syndrome (ACS) is an acute subset of heart disease that requires immediate treatment. ACS at a high prevalence in Indonesia. Homocysteine another product methionine metabolism. Hyperhomocysteinemia known to cause through several mechanisms, namely inducing oxidative stress, endothelial injury, and increased likelihood plaque rupture. Vitamin D hormone needed by the body comes from food or produced metabolism D. functions as anti-inflammatory, anti-thrombotic...

Chronic inflammation can have a considerable impact on the progression of glomerular and tubulointerstitial pathologies in chronic kidney disease (CKD). As an alternative to early detection inflammatory markers that are most important CKD, this study aims analyze association MLR, NLR, TNF-? with stages CKD. This cross-sectional conducted at Universitas Airlangga Hospital, Surabaya, Indonesia, mid-2023 involved 65 CKD subjects, consisting 5 according KDIGO guidelines. eGFR was calculated...

Introduction: oersistent high-risk HPV infection is the main cause of cervical cancer. Sexually active women are at risk infection, especially if they have multiple sexual partners. To detect HPV, DNA test can be conducted using menstrual blood samples as an alternative to cancer screening. Objective: this study aims analyze comparison results between screening with in sanitary pads and swabs single multi-partner sexual. Method: was analytical observational a cross-sectional design. The...

Aedes aegypti and albopictus are the primary secondary vectors, respectively, of dengue, most important arboviral disease in world. The aim this study was to detect serotype dengue viruses (DENV) vectors Ae. Surabaya, Indonesia. Between 2008 2015, 16,605 mosquitoes were collected 15 sub-districts Surabaya. dominant (90.9%), whereas few (9.1%). A total 330 pools adult subjected serotyping DENV by RT-PCR. DENV-1 (52.3%) frequently detected serotype, followed DENV-2 (40.3%), DENV-4 (4.6%),...

The characteristics of epidemic dengue often presented as periods hyperendemicity or the co-circulation multiple serotypes. Surabaya is an endemic city for Dengue virus (DENV) transmission. Previous study DENV distribution in 2008-2009 revealed predominance DENV-2. serotypes known to be dynamic and serotype may change through time. This aims determine follow circulation 2012. We recruited 154 denguesuspected patients attending Dr. Soetomo Hospital during February until August cases were...

Dengue virus (DENV) is a significant pathogen emerging worldwide as cause of infectious disease. Antidengue treatments are urgently required to control the emergence dengue. DENV mosquito-borne disease responsible for acute systemic diseases and serious health conditions. DENVs were distributed in tropical sub-tropical areas transmitted humans by Aedes agypty albopictus. vaccine or antiviral has not yet been clinically approved humans, even though there have great efforts toward this end....

Aim: The aim of this study was to investigate the toxicity goat bile in BALB/c mice since some Indonesian people consume raw gallbladder treat malaria and increase stamina. Materials Methods: Acute test done six groups using 100%, 50%, 25%, 12.5%, 6.75% negative control. death observed within 14 days. In subacute test, body weight hematology parameters on day 0 4 post-treatment were evaluated. closely for 28 days before plasma collection blood biochemistry evaluation. Results: Mild diarrhea...

Complications of Chronic Hepatitis B (CHB) infection are liver cirrhosis and Hepatocellular Carcinoma (HCC). Aspartate Aminotransferase (AST), Alanine (ALT), albumin may be used as indicators hepatocyte damage. This study aimed to determine differences between AST, ALT, in CHB patients without complications with HCC complications. An analytical cross-sectional was conducted March-May 2019 on 62 or using the total sampling method. AST ALT were calculated Siemens Dimension device IFCC The data...

Background: Prompt and reliable identification risk stratification in sepsis patients are needed to reduce the of mortality. Immature granulocytes (IG) mean platelet volume (MPV) considered as predictors 30-day mortality patients. This study aims analyze relationship between IG MPV with following emergency laparotomy due peritonitis. Materials Methods: In this observational retrospective study, IG, value, were obtained from medical records peritonitis who underwent an that met inclusion...

Background and Aim: Some individuals in Indonesia consume intact goat gallbladder to prevent treat malaria. The acute subacute toxicity tests of bile (GB) have shown mild diarrhea mice. Therefore, this study aimed evaluate the suppressive effect GB on parasitemia, splenomegaly, hepatomegaly, blood biochemistry assess liver kidney function BALB/c mice infected with Plasmodium berghei ANKA. Materials Methods: Fifty healthy were P. ANKA divided into five groups. Mice three groups administered...

Four serotypes of dengue viruses (DENV) 1–4 are mosquito-borne human pathogens that cause widespread epidemics withconsiderable morbidity and mortality. The aim this study was to evaluate the profile, which were circulating inSurabaya. This research has been carried out consisting 360 samples from patients with virus infections, according WorldHealth Organization (WHO) criteria. These sera collected Dr. Soetomo Hospital private laboratory in Surabayafrom 2008–2009. From samples, 68 (18.9%)...

Infeksi Virus Dengue (IVD) dibedakan menjadi infeksi primer dan sekunder berdasarkan respons antibodi yang dihasilkan. Infeksisekunder perlu dari karena umumnya menimbulkan manifestasi klinis berat. Uji hemaglutinasi inhibisisebagai baku emas untuk menentukan atau dirasa tidak praktis membutuhkan sepasang sera denganselang waktu cukup lama. Penelitian ini bertujuan mengetahui cut-off rasio IgG/IgM anti dengue denguesekunder dewasa di Surabaya. Subjek adalah pasien IVD dengan hasil NS1...

An urban park is potentially a source of vector-borne disease transmission due to it being natural and artificial mosquito breeding habitats combined with people's continuous presence. Thus, this study aims screen the occurrence knockdown-resistance (kdr) mutant alleles (V1016G F1534C) in populations collected from parks Surabaya, Indonesia. Cross sectional was conducted July 2019. A total 28 ovitraps were installed seven parks, having four each park. In total, 1,662 eggs collected, only 187...