A5068236884- Corneal Surgery and Treatments
- Corneal surgery and disorders
- Viral gastroenteritis research and epidemiology
- Ocular Surface and Contact Lens
- Wound Healing and Treatments
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Virus-based gene therapy research
- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- RNA and protein synthesis mechanisms
- Animal Virus Infections Studies
- Receptor Mechanisms and Signaling
- Surgical Sutures and Adhesives
- HIV Research and Treatment
- Advanced Glycation End Products research
- Respiratory viral infections research
- Reconstructive Surgery and Microvascular Techniques
- Viral Infections and Immunology Research
- Global Healthcare and Medical Tourism
- Medical and Biological Ozone Research
- Military and Defense Studies
- Organ Transplantation Techniques and Outcomes
- International Relations and Foreign Policy
- Hepatitis Viruses Studies and Epidemiology
World Health Organization Regional Office for Europe
2023
European Public Health Association
2023
Gladstone Institutes
2018-2022
University of California, San Francisco
2015-2022
University of Twente
2017
University of California San Francisco Medical Center
2015
Stanford University
2010-2012
VA Palo Alto Health Care System
2010
Inserm
2006-2008
Hôpitaux Universitaires de Strasbourg
2008
Abstract The alternate layer‐by‐layer (LBL) deposition of polycations and polyanions for the build up multilayered polyelectrolyte films is an original approach that allows preparation tunable, biologically active surfaces. resulting supramolecular nanoarchitectures can be functionalized with drugs, peptides, proteins, or DNA molecules are able to transfect cells in vitro. We monitor, first time, embedding a bioactive adenoviral (Ad) vector films. Ad efficiently adsorbs on poly( L...
Transparency of the human cornea is necessary for vision. Fuchs Endothelial Corneal Dystrophy (FECD) a bilateral, heritable degeneration corneal endothelium, and leading indication transplantation in developed countries. While early onset, rarer, form FECD has been linked to COL8A2 mutations, more common, late onset genetic mutations only minority cases. Epigenetic modifications that occur are unknown. Here, we report on compare DNA methylation landscape normal endothelial (CE) tissue CE...
Rotavirus (RV) predominantly replicates in intestinal epithelial cells (IEC), and "danger signals" released by these may modulate viral immunity. We have recently shown that human model IEC (Caco-2 cells) infected with rhesus-RV release a non-inflammatory group of immunomodulators includes heat shock proteins (HSPs) TGF-β1. Here we show both are part association membrane vesicles (MV) obtained from filtrated Caco-2 supernatants concentrated ultracentrifugation. These MV express markers...
Rotavirus replication and virulence are strongly influenced by virus strain host species. The rotavirus proteins VP3, VP4, VP7, NSP1, NSP4 have all been implicated in species restriction of replication; however, the mechanisms not fully determined. Simian (RRV) bovine (UK) rotaviruses distinctive capacities mouse extraintestinal organs such as biliary tract. Using reassortants between UK RRV, we previously demonstrated that differential these viruses embryonic fibroblasts is determined...
Abstract Homeostatic maintenance of corneal endothelial cells is essential for deturgescence and transparency. In Fuchs dystrophy (FECD), an accelerated loss dysfunction leads to progressively severe visual impairment. An abnormal accumulation extracellular matrix (ECM) a distinctive hallmark the disease, however molecular pathogenic mechanisms underlying this phenomenon are not fully understood. Here, we investigate genome-wide sequence-specific DNA methylation changes miRNA genes in...
ABSTRACT Rotavirus (RV) cell entry is an incompletely understood process, involving VP4 and VP7, the viral proteins composing outermost layer of nonenveloped RV triple-layered icosahedral particle (TLP), encasing VP6. can exist in three conformational states: soluble, cleaved spike, folded back. In order to better understand events leading entry, we established a detection system image input virus by monitoring rhesus (RRV) antigens VP4, VP6, VP7 at very early times postinfection. We provide...
This article analyses imaginaries of political decision makers the European Union in context 'refugee crisis' and interprets them according to theories integration – neofunctionalism liberal intergovernmentalism. Speeches, interviews, statements press releases 28 heads state government two Commissioners are analysed through a qualitative content analysis. The aim is derive prospects for from imaginaries. We found that expressed by largest group remain blurred without clarification which...
Abstract Following corneal injury, coordinated cellular and protein interactions occur at the wound site to restore tissue homeostasis. Regulation of this response is required prevent development chronic inflammation, abnormal neovascularization, fibrosis. The chemokine CCL2 its primary receptor CCR2 are key regulators inflammatory neovascular responses injury. In study, we investigated role macrophage-associated matrix metalloproteinase 12 (MMP12) in regulation after wounding. Using two...
Endocytosis has recently been implicated in rotavirus (RV) entry. We examined the role of Rabs, which regulate endosomal trafficking, during RV Several structural proteins neuraminidase-sensitive and -insensitive RVs colocalized with Rab5, an early endosome marker, but not Rab7, a late marker. Dominant-negative constitutively active mutants demonstrated that Rab5 Rab4 or Rab7 affects rhesus (RRV) infectivity. These data suggest RRV trafficking is confined to compartment requires Rab5.
Hepatitis C virus (HCV) is a human RNA encoding 10 proteins in single open reading frame. In the +1 frame, an 'alternate frame' (ARF) overlaps with core protein-encoding sequence and encodes ARF protein (ARFP). Here, we investigated molecular regulatory mechanisms of ARFP expression HCV target cells. Chimeric HCV-luciferase reporter constructs derived from infectious prototype isolate H77 were transfected into hepatocyte-derived cell lines. Translation initiation was most efficient at...
Significance Probabilistic bet hedging is a generalized diversification strategy to maximize fitness in unpredictable environments and has been proposed as an evolutionary basis for herpesvirus latency. However, the molecular mechanisms enabling probabilistic have remained elusive. Here, we find that human cytomegalovirus—a major cause of birth defects transplant failures—utilizes stochastic variability abundance protein packaged into individual viral particles enable between alternate states.
Purpose: Matrix metalloproteinases (MMPs) comprise a family of zinc-dependent endopeptidases involved in wound healing processes, including neovascularization and fibrosis. We assessed MMP protein expression levels diseased corneas patients requiring penetrating deep anterior lamellar keratoplasty. The purpose this study was to test the hypothesis that upregulation MMPs is positively associated with clinical corneal Methods: Protein nine individual were quantified simultaneously human...
Mononuclear phagocytes (MP) have central importance in innate immunity, inflammation, and fibrosis. Recruited MPs, such as macrophages, are plastic cells can switch from an inflammatory to a restorative phenotype during the healing process. However, role of MPs corneal wound is not completely understood. The purpose this study characterize kinetics recruited evaluate macrophage metalloelastase (MMP12) process, using vivo chemical injury model. Unwounded wounded corneas wild-type (WT)...
Abstract From microbes to cancers, drug-resistant ‘escape’ variants cause significant morbidity and mortality 1–7 . Here we present proof-of-concept that disruption of viral auto-regulatory (feedback) circuits strongly inhibits replication confers an extremely high barrier the evolution resistance. Using DNA duplexes, develop single-molecule ‘feedback-circuit disruptors’ interfere with transcriptional negative feedback in human herpesviruses (both Herpes Simplex Virus 1 Cytomegalovirus)...
ABSTRACT Probabilistic bet hedging, a strategy to maximize fitness in unpredictable environments by matching phenotypic variability environmental variability, is theorized account for the evolution of various fate-specification decisions, including viral latency. However, molecular mechanisms underlying bet-hedging remain unclear. Here, we report that large protein abundance within individual herpesvirus virion particles enables probabilistic hedging between replication and Super-resolution...