A5024082344- Plant Virus Research Studies
- Cytomegalovirus and herpesvirus research
- Bacteriophages and microbial interactions
- Plant and Fungal Interactions Research
- SARS-CoV-2 and COVID-19 Research
- interferon and immune responses
- Transgenic Plants and Applications
- Herpesvirus Infections and Treatments
- SARS-CoV-2 detection and testing
- Phytoplasmas and Hemiptera pathogens
- Legume Nitrogen Fixing Symbiosis
- HIV Research and Treatment
- Respiratory viral infections research
- Cocoa and Sweet Potato Agronomy
- Plant Disease Resistance and Genetics
- CRISPR and Genetic Engineering
- COVID-19 epidemiological studies
- COVID-19 Clinical Research Studies
- Plant-Microbe Interactions and Immunity
- T-cell and B-cell Immunology
- Viral Infections and Immunology Research
- Birth, Development, and Health
- Plant and Biological Electrophysiology Studies
- Reproductive Biology and Fertility
- Immune Cell Function and Interaction
Gladstone Institutes
2017-2023
University of California, San Francisco
2018-2020
University of California, Riverside
2011-2018
Stochastic fluctuations in gene expression ("noise") are often considered detrimental, but can also be exploited for benefit (e.g., dither). We show here that DNA base excision repair amplifies transcriptional noise to facilitate cellular reprogramming. Specifically, the protein Apex1, which recognizes both naturally occurring and unnatural modifications, while homeostatically maintaining mean levels. This amplified originates from shorter-duration, higher-intensity bursts generated by...
ABSTRACT Replication of the satellite RNA (satRNA) Cucumber Mosaic Virus is dependent on replicase proteins helper virus (HV). However, we recently demonstrated that like with Potato spindle tuber viroid (PSTVd), a satRNA associated strain Q (Q-satRNA) has propensity to localize in nucleus and generate multimers subsequently serve as templates for HV-dependent replication. But mechanism regulating nuclear importation Q-satRNA unknown. Here show mediated by bromodomain-containing host protein...
The high transmissibility of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a primary driver the COVID-19 pandemic. While existing interventions prevent disease, they exhibit mixed efficacy in preventing transmission, presumably due to their limited antiviral effects mucosa, whereas targeting sites viral replication might more effectively limit virus transmission. Recently, intranasally administered RNA-based therapeutic interfering particles (TIPs) were reported suppress...
Significance Probabilistic bet hedging is a generalized diversification strategy to maximize fitness in unpredictable environments and has been proposed as an evolutionary basis for herpesvirus latency. However, the molecular mechanisms enabling probabilistic have remained elusive. Here, we find that human cytomegalovirus—a major cause of birth defects transplant failures—utilizes stochastic variability abundance protein packaged into individual viral particles enable between alternate states.
Significance How biological systems robustly sustain differentiated states is under active study. Here, we find that human cytomegalovirus, a herpesvirus major cause of birth defects, encodes transcriptional positive-feedback loop temporally extends transient activation signals carried in the incoming viral particle to lytic expression cycle. Attenuation this feedback severely impacts virus fitness, suggesting new antiviral target may extend other herpesviruses.
Positive-strand RNA viruses are known to rearrange the endomembrane network make it more conducive for replication, maturation, or egress. Our previous transmission electron microscopic (TEM) analysis showed that ectopic expression of wild-type (wt) capsid protein (CP) Brome mosaic virus (BMV) has an intrinsic property modifying endoplasmic reticulum (ER) induce vesicles similar those present in wt BMV infection. In this study, we evaluated functional significance CP-mediated vesicle...
Herpes simplex virus-1 (HSV-1) is a significant human pathogen. Upon infection, HSV-1 expresses its immediate early (IE) genes, and the IE transcription factor ICP4 (infectious cell protein-4) plays pivotal role in initiating downstream gene-expression cascade. Using live-cell time-lapse fluorescence microscopy, we quantitatively monitored expression of individual cells after infection. We find that extrinsic stimuli can accelerate kinetics without increasing protein levels. The accelerated...
In viruses with positive-sense RNA genomes pathogenic to humans, animals and plants, progeny encapsidation into mature stable virions is a cardinal phase during establishment of infection in given host. Consequently, study deciphers the information regarding know-how mechanism regulating virus assembly form infectious virions. Such vital formulating novel methods curbing spread disease control. Virus can be studied vivo vitro. Genome highly regulated selective process involving...
Pathogenic or non-pathogenic small (17 to 30 nt) and long (>200 non-coding RNAs (ncRNAs) have been implicated in the regulation of gene expression at transcriptional, post-transcriptional epigenetic level by interacting with host proteins. However, lack suitable experimental system precludes identification evaluation functional significance proteins ncRNAs. In this study, we present a first report on application riboproteomics identify small, highly pathogenic, noncoding satellite RNA...
Abstract The high transmissibility of SARS-CoV-2 is a primary driver the COVID-19 pandemic. While existing interventions prevent severe disease, they exhibit mixed efficacy in preventing transmission, presumably due to their limited antiviral effects respiratory mucosa, whereas targeting sites viral replication might more effectively limit virus transmission. Recently, intranasally administered RNA-based therapeutic interfering particles (TIPs) were reported suppress replication, barrier...
Abstract From microbes to cancers, drug-resistant ‘escape’ variants cause significant morbidity and mortality 1–7 . Here we present proof-of-concept that disruption of viral auto-regulatory (feedback) circuits strongly inhibits replication confers an extremely high barrier the evolution resistance. Using DNA duplexes, develop single-molecule ‘feedback-circuit disruptors’ interfere with transcriptional negative feedback in human herpesviruses (both Herpes Simplex Virus 1 Cytomegalovirus)...
ABSTRACT Probabilistic bet hedging, a strategy to maximize fitness in unpredictable environments by matching phenotypic variability environmental variability, is theorized account for the evolution of various fate-specification decisions, including viral latency. However, molecular mechanisms underlying bet-hedging remain unclear. Here, we report that large protein abundance within individual herpesvirus virion particles enables probabilistic hedging between replication and Super-resolution...
Introduction: Thyroid dysfunctions are among the most common diseases in women of reproductive age group. hormone receptors present on oocytes which indicate that thyroid hormones may influence ovarian functions. Serum AntiMüllerian Hormone (AMH) is secreted from growing granulosa cells ovaries. AMH concentrations used to evaluate reserve females. Aim: To find association between reserve, measured by concentration and functions with infertility. Materials Methods: This cross-sectional study...