A5100746316- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Mesenchymal stem cell research
- RNA modifications and cancer
- RNA Research and Splicing
- MicroRNA in disease regulation
- Traditional Chinese Medicine Studies
- Single-cell and spatial transcriptomics
- Traditional Chinese Medicine Analysis
- Cancer-related molecular mechanisms research
- Immune Cell Function and Interaction
- Cancer Genomics and Diagnostics
- T-cell and B-cell Immunology
- Metabolomics and Mass Spectrometry Studies
- Genetic Associations and Epidemiology
- Plant Pathogens and Resistance
- Zebrafish Biomedical Research Applications
- Bioinformatics and Genomic Networks
- Protein Structure and Dynamics
- Genetics and Neurodevelopmental Disorders
- Hematopoietic Stem Cell Transplantation
- Pancreatic function and diabetes
- Renin-Angiotensin System Studies
Eli Lilly (United States)
2025
Fudan University
2024-2025
State Key Laboratory of Genetic Engineering
2025
Zhongshan Hospital
2025
Shenzhen Bay Laboratory
2020-2024
Ningbo University
2024
Beijing Sport University
2020-2024
Central South University
2019-2024
University of Wisconsin–Madison
2012-2024
Renji Hospital
2024
Mutations in the human LIS1 gene cause smooth brain disease classical lissencephaly. To understand underlying mechanisms, we conducted situ live cell imaging analysis of function throughout entire radial migration pathway. In utero electroporation small interference RNA and short hairpin dominant negative dynactin cDNAs caused a dramatic accumulation multipolar progenitor cells within subventricular zone embryonic rat brains. This effect resulted from complete failure progression to...
Abstract Intrinsically disordered proteins (IDPs) are associated with various diseases and have been proposed as promising drug targets. However, conventional structure-based approaches cannot be applied directly to IDPs, due their lack of ordered structures. Here, we describe a novel computational approach virtually screen for compounds that can simultaneously bind different IDP conformations. The test system used c-Myc, an oncoprotein containing basic helix-loop-helix-leucine zipper...
Stochastic fluctuations in gene expression ("noise") are often considered detrimental, but can also be exploited for benefit (e.g., dither). We show here that DNA base excision repair amplifies transcriptional noise to facilitate cellular reprogramming. Specifically, the protein Apex1, which recognizes both naturally occurring and unnatural modifications, while homeostatically maintaining mean levels. This amplified originates from shorter-duration, higher-intensity bursts generated by...
One key step in producing insulin-secreting cells from human embryonic stem (hES) is the generation of pancreatic and duodenal homeobox gene 1 (PDX1)-expressing progenitor cells. All-trans retinoic acid (RA) has important roles pancreas development widely used to induce differentiation ES When RA was added directly activin A-induced hES cells, <20% were positive for marker PDX1, whereas other mainly hepatic We found that when replated seeded at low cell densities, addition induced...
Despite intensive breeding efforts, potato late blight, caused by the oomycete pathogen Phytophthora infestans, remains a threat to production worldwide because newly evolved strains have consistently overcome major resistance genes. The RB gene, derived from wild species Solanum bulbocastanum, confers most P. infestans through recognition of members effector family IPI-O. While majority IPI-O proteins are recognized elicit (e.g. IPI-O1, IPI-O2), some able elude detection IPI-O4). In...
Intrinsically disordered proteins (IDPs) were found to be widely associated with human diseases and may serve as potential drug design targets. However, targeting IDPs is still in the very early stages. Progress usually achieved using experimental screening; however, structural disorder of makes it difficult characterize their interaction ligands experiments alone. To better understand structure interactions small molecule ligands, we performed extensive simulations on c-Myc370–409 peptide...
Tissue-resident stem cell senescence leads to exhaustion, which is a major cause of physiological and pathological ageing. Stem cell-derived extracellular vesicles (SC-EVs) have been reported in preclinical studies possess therapeutic potential for diverse diseases. However, whether SC-EVs can rejuvenate senescent tissue cells prevent age-related disorders still remains unknown. Here, we show that chronic application human embryonic small (hESC-sEVs) rescues the function bone marrow...
The phenomenon of T Cell exhaustion (TEX) entails a progressive deterioration in the functionality cells within immune system during prolonged conflicts with chronic infections or tumors. In context ovarian cancer immunotherapy, development, and outcome treatment are closely linked to T-cell exhaustion. Hence, gaining an in-depth understanding features TEX microenvironment is paramount importance for management OC patients. To this end, we leveraged single-cell RNA data from perform...
Significance Myelodysplastic syndrome (MDS) represents a common hematopoietic disease, often in elderly patients, with heterogeneous clinical phenotypes and complex disease mechanisms. Here, we report on characteristic genome lesions, clonal architecture, distinct tumor clone expansion patterns group of patients refractory anemia excess blasts, the MDS subtype highest propensity to acute myeloid leukemia. An integrative gene mutation analysis 196 different subtypes allowed regulatory network...
Abstract Mounting evidence indicates that microRNAs (miRNAs) are involved in multiple processes of osteogenic differentiation. MicroRNA-101 (miR-101), identified as a tumor suppressor, has been implicated the pathogenesis several types cancer. However, expression miR-101 and its roles differentiation human bone marrow-derived mesenchymal stem cells (hBMSCs) remain unclear. We found level was significantly increased during hBMSCs. MiR-101 depletion suppressed differentiation, whereas...
To study the effects of acute ammonia stress on poisoning reaction hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatu ), 300 healthy juveniles with an initial body weight 51.4 ± 2.57 g were selected for experiment using a half-lethal concentration 24, 48, 72, and 96 h triplicate. The results show that (1) concentrations 39.5 mg/L 24 h, 27.3 48 26.5 72 25.0 safe was 2.50 mg/L. (2) biochemical indices serum livers groupers fluctuated under ( P &lt; 0.05), contents cortisol...
Many psychiatric disorders exhibit sex differences, but the underlying mechanisms remain poorly understood. We analyzed transcriptomics data from 2160 postmortem adult prefrontal cortex brain samples PsychENCODE consortium in a sex-stratified study design. compared of patients with schizophrenia (SCZ), bipolar disorder (BD), and autism spectrum (ASD) control brains individuals without known history disease. found that females SCZ, BD, ASD showed higher burden transcriptomic dysfunction than...