A5066487783- Pain Mechanisms and Treatments
- Single-cell and spatial transcriptomics
- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Autism Spectrum Disorder Research
- Genetics and Neurodevelopmental Disorders
- Cell Image Analysis Techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Pharmacological Effects of Natural Compounds
- Synthesis and pharmacology of benzodiazepine derivatives
- Immune cells in cancer
- Pancreatic function and diabetes
- Cancer Treatment and Pharmacology
- Alzheimer's disease research and treatments
- CRISPR and Genetic Engineering
- Obstructive Sleep Apnea Research
- Immune Response and Inflammation
- Pain Management and Opioid Use
- Microtubule and mitosis dynamics
- Wnt/β-catenin signaling in development and cancer
- Cancer, Stress, Anesthesia, and Immune Response
- Neurotransmitter Receptor Influence on Behavior
- Gene Regulatory Network Analysis
- Pharmacological Effects and Toxicity Studies
- Biochemical Analysis and Sensing Techniques
The University of Texas Southwestern Medical Center
2025
Southwestern Medical Center
2025
The University of Texas Medical Branch at Galveston
2023-2024
Rutgers, The State University of New Jersey
2021-2024
Johnson University
2021-2023
Rutgers Sexual and Reproductive Health and Rights
2021-2023
Baylor College of Medicine
2020-2021
University of Toronto
2016-2019
Istituto Neurologico Mediterraneo
2012-2017
Canada Research Chairs
2016-2017
Abstract Cortical neurons exhibit extreme diversity in gene expression as well morphological and electrophysiological properties 1,2 . Most existing neural taxonomies are based on either transcriptomic 3,4 or morpho-electric 5,6 criteria, it has been technically challenging to study both aspects of neuronal the same set cells 7 Here we used Patch-seq 8 combine patch-clamp recording, biocytin staining, single-cell RNA sequencing more than 1,300 adult mouse primary motor cortex, providing a...
Cortical neurons exhibit astounding diversity in gene expression as well morphological and electrophysiological properties. Most existing neural taxonomies are based on either transcriptomic or morpho-electric criteria, it has been technically challenging to study both aspects of neuronal the same set cells. Here we used Patch-seq combine patch-clamp recording, biocytin staining, single-cell RNA sequencing over 1300 adult mouse motor cortex, providing a comprehensive annotation almost all...
Pharmacological activation of type-2 metabotropic glutamate receptors (mGlu2 receptors) causes analgesia in experimental models inflammatory and neuropathic pain. Presynaptic mGlu2 are activated by the released from astrocytes means cystine/glutamate antiporter (System x(c)(-) or Sx(c)(-)). We examined analgesic activity Sx(c)(-) activator, N-acetyl-cysteine (NAC), mice developing pain.A single injection NAC (100 mg/kg, i.p.) reduced nocifensive behavior second phase formalin test....
Excitatory synaptic transmission in central synapses is modulated by serotonin (5-HT). The anterior cingulate cortex (ACC) an important cortical region for pain perception and emotion. ACC neurons receive innervation of projecting serotonergic nerve terminals from raphe nuclei, but the possible effect 5-HT on excitatory has not been investigated. In present study, we investigated role glutamate neurotransmission slices adult mice. Bath application produced dose-dependent inhibition evoked...
ABSTRACT We report the generation of a multimodal cell census and atlas mammalian primary motor cortex (MOp or M1) as initial product BRAIN Initiative Cell Census Network (BICCN). This was achieved by coordinated large-scale analyses single-cell transcriptomes, chromatin accessibility, DNA methylomes, spatially resolved morphological electrophysiological properties, cellular resolution input-output mapping, integrated through cross-modal computational analysis. Together, our results advance...
Abstract Electroencephalographic (EEG) recordings in individuals with Fragile X Syndrome (FXS) and the mouse model of FXS ( Fmr1 KO) display cortical hyperexcitability at rest, as well deficits sensory-driven network synchrony. A form circuit is observed ex vivo slices KO mice prolonged persistent activity, or Up, states. It unknown if mechanisms that cause Up states contribute to FXS-relevant EEG phenotypes. Here we examined role endocannabinoids (eCB) resting phenotypes awake mice....
BackgroundL-acetylcarnitine, a drug marketed for the treatment of chronic pain, causes analgesia by epigenetically up-regulating type-2 metabotropic glutamate (mGlu2) receptors in spinal cord. Because epigenetic mechanisms are typically long-lasting, we hypothesized that could outlast duration L-acetylcarnitine models inflammatory and neuropathic pain. ResultsA seven-day with (100 mg/kg, once day, i.p.) produced an antiallodynic effect complete Freund adjuvant mouse model...
Cortical areas including the anterior cingulate cortex (ACC) play critical roles in different types of chronic pain. Most previous studies focus on sensory inputs from somatic areas, and less information about plastic changes for visceral In this study, pain animal model was established by injection with zymosan into colon adult male C57/BL6 mice. Whole cell patch-clamp recording, behavioral tests, western blot, Cannulation ACC microinjection were employed to explore role adenylyl cyclase 1...
The Par3 polarity protein is critical for subcellular compartmentalization in different developmental processes. Variants of
Anxiety disorder is a state of mental discomfort while acute anxiety induces an enhancement vigilance/arousal or increased anxious responses. Most the previous studies investigated basic mechanisms for anxiety, less information available prolonged repetitive anxiety. In present study, we wanted to examine possible molecular behavioral after repeated exposures. Performing paradigm five sessions elevated plus-maze (EPM), show that exposure EPM long-lasting causing gradual increase anxiolytic...
Chemotherapy-induced peripheral neuropathy (CIPN) affects ∼68% of patients undergoing chemotherapy, causing debilitating neuropathic pain and reducing quality life. Cisplatin is a commonly used platinum-based chemotherapeutic drug known to cause CIPN, possibly by oxidative stress damage primary sensory neurons. Metabotropic glutamate receptors (mGluRs) are widely hypothesized be involved in processing mitigation. Meclizine an H1 histamine receptor antagonist have neuroprotective effects,...
Abstract Central nervous system (CNS) control of metabolism plays a pivotal role in maintaining energy homeostasis. Glucagon-like peptide-1 (GLP-1, encoded by Gcg ), secreted distinct population neurons located within the nucleus tractus solitarius (NTS), suppresses feeding through projections to multiple brain targets 1-3 . Although GLP-1 analogs are proven clinically effective treating type 2 diabetes and obesity 4 , mechanisms action remain unclear. Here, we investigate involvement...
Cocaine use disorder (CUD) is a prevalent neuropsychiatric with few existing treatments. Thus, there an unmet need for the identification of new pharmacological targets CUD. Previous studies using environmental enrichment versus isolation paradigms have found that latter induces increased cocaine self-administration correlative increases in excitability medium spiny neurons (MSN) nucleus accumbens shell (NAcSh). Expanding upon these findings, we sought present investigation to elucidate...
Abstract Mutations in many synaptic genes are associated with autism spectrum disorders (ASDs), suggesting that dysfunction is a key driver of ASD pathogenesis. Among these mutations, the R451C-substitution NLGN3 gene encodes postsynaptic adhesion molecule Neuroligin-3 noteworthy because it was first specific mutation linked to ASDs. In mice, corresponding Nlgn3 R451C-knockin recapitulates social interaction deficits patients and produces abnormalities, but impact R451C-mutation on human...
Microglia are critical for brain development and play a central role in Alzheimer’s disease (AD) etiology. Down syndrome (DS), also known as trisomy 21, is the most common genetic origin of intellectual disability risk factor AD. Surprisingly, little information available on impact human chromosome 21 (Hsa21) microglia DS AD (DSAD). Using our new induced pluripotent stem cell (iPSC)-based microglia-containing cerebral organoid chimeric mouse models, here we report that exhibit enhanced...
Abstract Microglia are critical for brain development and play a central role in Alzheimer’s disease (AD) etiology. Down syndrome (DS), also known as trisomy 21, is the most common genetic origin of intellectual disability risk factor AD. Surprisingly, little information available on impact human chromosome 21 (Hsa21) microglia DS AD (DSAD). Using our new induced pluripotent stem cell (iPSC)-based microglia-containing cerebral organoid chimeric mouse models, here we report that exhibit...
Summary The Par3 polarity protein is critical for subcellular compartmentalization in different developmental processes. Variants of PARD3 , which encodes PAR3, are associated with intelligence and neurodevelopmental disorders. However, the role glutamatergic synapse formation cognitive functions vivo remains unknown. Here, we show that forebrain conditional knockout leads to an increase long, thin dendritic spines without significantly impacting mushroom . In addition, observed a decrease...
Abstract Chemotherapy-induced peripheral neuropathy (CIPN) affects about 68% of patients undergoing chemotherapy and causes severe neuropathic pain which is debilitating health problem greatly reduces quality life. Cisplatin a commonly used platinum-based chemotherapeutic drug known to cause CIPN, possibly by causing oxidative stress damage primary sensory neurons. Metabotropic glutamate receptors (mGluRs) are widely hypothesized be involved in processing. Meclizine an H1 histamine receptor...