A5052377980- Pain Mechanisms and Treatments
- Nitric Oxide and Endothelin Effects
- Circadian rhythm and melatonin
- Exercise and Physiological Responses
- Neuropeptides and Animal Physiology
- Ion channel regulation and function
- Olfactory and Sensory Function Studies
- Mast cells and histamine
- Synthesis and pharmacology of benzodiazepine derivatives
- Spaceflight effects on biology
- Neuroscience and Neural Engineering
- Receptor Mechanisms and Signaling
- Ion Channels and Receptors
- Stress Responses and Cortisol
- Anesthesia and Pain Management
- Neuroscience and Neuropharmacology Research
- Cellular Mechanics and Interactions
- Cancer Treatment and Pharmacology
- Advanced Fluorescence Microscopy Techniques
- Sleep and Wakefulness Research
- Thermoregulation and physiological responses
- Asthma and respiratory diseases
- Menopause: Health Impacts and Treatments
- Urticaria and Related Conditions
- Heart Rate Variability and Autonomic Control
University of Miami
2023-2024
University of Shizuoka
2016-2023
The University of Texas Health Science Center at San Antonio
2021-2023
National Medical Research Center of Dentistry and Maxillofacial Surgery
2021-2022
Previous studies have shown that infiltration of capsaicin into the surgical site can prevent incision-induced spontaneous pain like behaviors and heat hyperalgesia. In present study, we aimed to monitor primary sensory neuron Ca 2+ activity in intact dorsal root ganglia (DRG) using Pirt-GCaMP3 male female mice pretreated with or vehicle before plantar incision. Intraplantar injection (0.05%) significantly attenuated pain, mechanical, hypersensitivity after The response vivo DRG situ spinal...
Chemotherapy-induced peripheral neuropathy (CIPN) affects ∼68% of patients undergoing chemotherapy, causing debilitating neuropathic pain and reducing quality life. Cisplatin is a commonly used platinum-based chemotherapeutic drug known to cause CIPN, possibly by oxidative stress damage primary sensory neurons. Metabotropic glutamate receptors (mGluRs) are widely hypothesized be involved in processing mitigation. Meclizine an H1 histamine receptor antagonist have neuroprotective effects,...
Crisaborole, a phosphodiesterase 4 (PDE4) inhibitor, has been approved for the treatment of mild to moderate atopic dermatitis. Atopic dermatitis is often associated with increased pain. Using mouse model, this study investigated whether crisaborole suppresses pain and potential mechanisms underlying it. The model was developed by repeatedly applying MC903. MC903-treated mice had spontaneous scratching (itch-related behaviour) wiping behaviour (pain-related behaviour). Crisaborole topically...
High K+-induced contraction of arterial smooth muscle is thought to be mediated by membrane depolarization and subsequent activation voltage-dependent Ca2+ channels (VDCCs). In line with this, this study found that induced 80 mM K+ was almost abolished nifedipine (1 µM), a VDCC inhibitor, in isolated rat aorta, markedly suppressed the iliac artery. However, µM) only partially high tail The contractions remaining arteries were further reduced non-selective cation channel (NSCC) inhibitors,...
Abstract Detection of somatosensory inputs requires conversion external stimuli into electrical signals by activation primary sensory neurons. The mechanisms which heterogeneous neurons encode different remains unclear. In vivo dorsal root ganglia (DRG) imaging using genetically-encoded Ca 2+ indicators (GECIs) is currently the best technique for this purpose providing an unprecedented spatial and populational resolution. It permits simultaneous >1800 neurons/DRG in live mice. However,...
Previous our study found that improvement of skin blood flow associated with neuropathic pain using vasodilators is useful for alleviation pain. In this study, we aimed to elucidate the mechanism underlying enhanced vasorelaxation induced by vasodilators, which increase cAMP and cyclic guanosine monophosphate (cGMP), in chronic constriction injury model rat. We assessed effect measurement isometric contraction isolated plantar artery from sciatic nerve rats. Nifedipine, a voltage-dependent...
The central amygdala (CeA) is a crucial hub in the processing of affective itch, containing diverse array neuronal populations. Among these components, Neuropeptide Y (NPY) and its receptors, such as NPY2R, affect various physiological psychological processes. Despite this broad impact, precise role NPY2R
Our previous studies have shown that phenylephrine-induced contraction of cutaneous arteries is primarily mediated via α1A-adrenoceptors, but not α1D-adrenoceptors generally mediate vascular contraction, and the larger part induced in a voltage-dependent Ca2+ channel (VDCC)-independent manner. Here, we investigated mechanism underlying smaller α1A-adrenoceptor-mediated i.e., VDCC-dependent one, arteries. Isometric was measured with wire myograph endothelium-denuded tail iliac arterial rings...
Benzodiazepines (BDZs) and non-BDZ drugs are associated with side effects such as headaches, dizziness, palpitations. Therefore, we evaluated whether these have direct vasorelaxation onphenylephrine-contracted arteries isolated from Wistar rats using myography. The binding assay of the for GABAAreceptors in rat brain was performed [3H]flunitrazepam an agonist.
Abstract Chemotherapy-induced peripheral neuropathy (CIPN) affects about 68% of patients undergoing chemotherapy and causes severe neuropathic pain which is debilitating health problem greatly reduces quality life. Cisplatin a commonly used platinum-based chemotherapeutic drug known to cause CIPN, possibly by causing oxidative stress damage primary sensory neurons. Metabotropic glutamate receptors (mGluRs) are widely hypothesized be involved in processing. Meclizine an H1 histamine receptor...
Abstract Rehabilitation from alcohol addiction or abuse is challenging due to withdrawal symptoms. Headache a severe symptom that frequently contributes rehabilitation failure. Despite the need for treating withdrawal-induced headache, there no appropriate therapeutic option available. Development of improved therapeutics will depend on obtaining clearer understanding headache pain mechanisms. Here, we show mast cell-specific receptor MrgprB2 controls development headache. Withdrawing...
Abstract After surgeries, especially thoracotomy incision, patients develop unbearable pain. Opioids are used for reducing pain but often cause serious side effects. Previously, we found that capsaicin pretreatment of the incision area alleviated spontaneous and thermal in a postoperative animal model. In present study, aimed to monitor primary sensory neuron Ca 2+ activity vivo dorsal root ganglia (DRG) model using Pirt-GCaMP3 treated with or controls. Intraplantar injection (0.05%)...
Rehabilitation from alcohol addiction or abuse is challenging due to withdrawal symptoms. Headache a severe symptom that frequently contributes rehabilitation failure. Despite the need for treating withdrawal-induced headache, there no appropriate therapeutic option available. Development of improved therapeutics will depend on obtaining clearer understanding headache pain mechanisms. Here, we show mast cell-specific receptor MrgprB2 controls development headache. Withdrawing...
Abstract Detection of somatosensory inputs requires conversion external stimuli into electrical signals by activation primary sensory neurons. The mechanisms which heterogeneous neurons encode different remains unclear. In vivo dorsal root ganglia (DRG) imaging using genetically-encoded Ca 2+ indicators (GECIs) is currently the best technique for this purpose mapping neuronal function in DRG circuits providing an unprecedented spatial and populational resolution. It permits simultaneous...