A5100693453- Neural dynamics and brain function
- Neuroscience and Neural Engineering
- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neuropharmacology Research
- Memory and Neural Mechanisms
- Tryptophan and brain disorders
- Neurogenesis and neuroplasticity mechanisms
- Alzheimer's disease research and treatments
- Advanced Memory and Neural Computing
- Bipolar Disorder and Treatment
- Cellular Mechanics and Interactions
- Ion Channels and Receptors
- Signaling Pathways in Disease
- Endoplasmic Reticulum Stress and Disease
- Advanced materials and composites
- Cancer, Hypoxia, and Metabolism
- Zebrafish Biomedical Research Applications
- Peroxisome Proliferator-Activated Receptors
- Autophagy in Disease and Therapy
- High Entropy Alloys Studies
- Mitochondrial Function and Pathology
- Click Chemistry and Applications
- Metal and Thin Film Mechanics
- Pluripotent Stem Cells Research
- Nerve injury and regeneration
Stanford University
2009-2024
Stanford Medicine
2021-2023
GlaxoSmithKline (United States)
2009-2022
South College
2022
National Institute of Biomedical Imaging and Bioengineering
2015-2019
Sun Yat-sen University
2017-2018
National Institutes of Health
2015
GlaxoSmithKline (United Kingdom)
2014
Air Force Medical University
2013
Xijing Hospital
2013
Many patients with temporal lobe epilepsy display neuron loss in the dentate gyrus. One potential epileptogenic mechanism is of GABAergic interneurons and inhibitory synapses granule cells. Stereological techniques were used to estimate numbers gephyrin-positive punctae gyrus, which reduced short-term (5 days after pilocarpine-induced status epilepticus) but later rebounded beyond controls epileptic rats. electron micrographs serial sections processed for postembedding GABA-immunoreactivity....
Article6 May 2019free access Source DataTransparent process Anoctamin 8 tethers endoplasmic reticulum and plasma membrane for assembly of Ca2+ signaling complexes at the ER/PM compartment Archana Jha The Epithelial Signaling Transport Section, National Institute Dental Craniofacial Research, Institutes Health, Bethesda, MD, USA Search more papers by this author Woo Young Chung Laura Vachel Jozsef Maleth orcid.org/0000-0001-5768-3090 First Department Medicine, University Szeged, Hungary Sarah...
Activation of the inositol-requiring enzyme-1 alpha (IRE1α) protein caused by endoplasmic reticulum stress results in homodimerization N-terminal luminal domains, autophosphorylation cytoplasmic kinase and conformational changes to endoribonuclease (RNase) which render them functional can lead splicing X-box binding 1 (XBP 1) mRNA. Herein, we report first crystal structures portion a human phosphorylated IRE1α dimer complex with...
A series of cardiac troponin I-interacting kinase (TNNI3K) inhibitors arising from 3-((9H-purin-6-yl)amino)-N-methyl-benzenesulfonamide (1) is disclosed along with fundamental structure-function relationships that delineate the role each element 1 for TNNI3K recognition. An X-ray structure bound to confirmed its Type I binding mode and used rationalize structure-activity relationship employed design potent, selective, orally bioavailable inhibitors. Identification 7-deazapurine heterocycle...
Abstract Indoleamine‐2,3‐dioxygenase 1 (IDO1) is a heme‐containing enzyme that catalyzes the rate‐limiting step in kynurenine pathway of tryptophan (TRP) metabolism. As it an inflammation‐induced immunoregulatory enzyme, pharmacological inhibition IDO1 activity currently being pursued as potential therapeutic tool for treatment cancer and other disease states. such, detailed understanding mechanism action inhibitors with various mechanisms great interest. Comparison apo‐form‐binding...
Recent advances in neural stem cell (NSC) transplantation have led to an inspiring progress alleviating central nervous system (CNS) damages and restoring brain functions from diseases or injuries. One challenge of NSC is directed differentiation transplanted NSCs into desired neuronal subtypes, such as neurons, compensate the adverse impact injury; another lies lack tools noninvasively monitor dynamics after vivo. In this study, we developed a polymer nanovehicle for morphogen sustained...
E1A binding protein (p300) and CREB (CBP) are two highly homologous multidomain histone acetyltransferases. These proteins involved in many cellular processes by acting as coactivators of a large number transcription factors. Dysregulation p300/CBP has been found variety cancers other diseases, inhibition shown to decrease Myc expression. Herein, we report the identification series potent, proline-based small-molecule acetyltransferase (HAT) inhibitors using DNA-encoded library technology...
Fast electrical signaling in dendrites is central to neural computations that support adaptive behaviors. Conventional techniques lack temporal and spatial resolution the ability track underlying membrane potential dynamics present across complex three-dimensional dendritic arbor vivo. Here, we perform fast two-photon imaging of somatic single pyramidal cells CA1 region mouse hippocampus during awake behavior. We study subthreshold suprathreshold events throughout vivo by combining voltage...
Identification of kinase, especially protein modulators through high-throughput screening (HTS) has become a common strategy for drug discovery programs in both academia and the pharmaceutical industry. There are number platform technologies that can be used measuring kinase activities. However, there is none fits all criteria terms sensitivity, ATP tolerance, robustness, throughput, cost-effectiveness. Therefore, development homogeneous robust HTS assay some targets still challenging. We...
Abstract Neuronal spiking activity is routinely recorded using genetically encoded calcium indicators (GECIs), but imaging limited in temporal resolution and does not report subthreshold voltage changes. Genetically (GEVIs) offer better sensitivity, spike detection with fast GEVIs has required specialized equipment. Here, we the ASAP4 subfamily of that brighten response to membrane depolarization, inverting fluorescence-voltage relationship previous ASAP GEVIs. Two variants, ASAP4b ASAP4e,...
Homozygous tau knockout (Mapt-/-) mice develop age-dependent dopaminergic (DA) neuronal loss in the substantia nigra (SN) and ventral tegmental area (VTA), supporting an important function of maintaining survival midbrain neurons (mDANs) during aging. However, it remains to be determined whether microtubule-associated protein regulates differentiation mDANs embryonic developmental stages. Here, we show that haploinsufficiency postnatal day 0 (P0) heterozygous (Mapt+/-) pups, but not a...
ABSTRACT Indoleamine-2,3-dioxygenase 1 (IDO1) is a heme-containing enzyme that catalyzes the rate-limiting step in kynurenine pathway of tryptophan (TRP) metabolism. As an inflammation-induced immunoregulatory enzyme, pharmacological inhibition IDO1 activity currently being pursued as potential therapeutic tool for treatment cancer and other disease states. such, detailed understanding mechanism action established novel inhibitors remains great interest. Comparison newly-developed inhibitor...
Abstract Detection of somatosensory inputs requires conversion external stimuli into electrical signals by activation primary sensory neurons. The mechanisms which heterogeneous neurons encode different remains unclear. In vivo dorsal root ganglia (DRG) imaging using genetically-encoded Ca 2+ indicators (GECIs) is currently the best technique for this purpose providing an unprecedented spatial and populational resolution. It permits simultaneous >1800 neurons/DRG in live mice. However,...
Fast electrical signaling in dendrites is central to neural computations that support adaptive behaviors. Conventional techniques lack temporal and spatial resolution the ability track underlying membrane potential dynamics present across complex three-dimensional dendritic arbor vivo . Here, we perform fast two-photon imaging of somatic single pyramidal cells CA1 region mouse hippocampus during awake behavior. We study subthreshold suprathreshold events throughout by combining voltage with...
Abstract Detection of somatosensory inputs requires conversion external stimuli into electrical signals by activation primary sensory neurons. The mechanisms which heterogeneous neurons encode different remains unclear. In vivo dorsal root ganglia (DRG) imaging using genetically-encoded Ca 2+ indicators (GECIs) is currently the best technique for this purpose mapping neuronal function in DRG circuits providing an unprecedented spatial and populational resolution. It permits simultaneous...