A5074129382- Neuroscience and Neuropharmacology Research
- Retinal Development and Disorders
- Memory and Neural Mechanisms
- Photoreceptor and optogenetics research
- Neural dynamics and brain function
- Zebrafish Biomedical Research Applications
- Single-cell and spatial transcriptomics
- Polymer Surface Interaction Studies
- CRISPR and Genetic Engineering
- Neurobiology and Insect Physiology Research
- Mitochondrial Function and Pathology
- Autophagy in Disease and Therapy
- Neuroscience and Neural Engineering
- Nicotinic Acetylcholine Receptors Study
- Connexins and lens biology
- Obstructive Sleep Apnea Research
- Protein Structure and Dynamics
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- biodegradable polymer synthesis and properties
- Cerebrovascular and Carotid Artery Diseases
- Blood Coagulation and Thrombosis Mechanisms
- Advanced Polymer Synthesis and Characterization
- Endoplasmic Reticulum Stress and Disease
- Protease and Inhibitor Mechanisms
- Cellular transport and secretion
Columbia University
2019-2025
University of British Columbia
2020-2024
Brain (Germany)
2023
Wayne State College
2022
City University of New York
2019-2021
City College of New York
2017-2021
Canadian Blood Services
2021
Dendritic calcium signaling is central to neural plasticity mechanisms that allow animals adapt the environment. Intracellular release (ICR) from endoplasmic reticulum has long been thought shape these mechanisms. However, ICR not investigated in mammalian neurons vivo. We combined electroporation of single CA1 pyramidal neurons, simultaneous imaging dendritic and somatic activity during spatial navigation, optogenetic place field induction, acute genetic augmentation cytosolic impact reveal...
Abstract Neuronal mitochondria play important roles beyond ATP generation, including Ca 2+ uptake, and therefore have instructive in synaptic function neuronal response properties. Mitochondrial morphology differs significantly between the axon dendrites of a given subtype, but CA1 pyramidal neurons (PNs) hippocampus, within dendritic arbor also display remarkable degree subcellular, layer-specific compartmentalization. In these neurons, ranges from highly fused elongated apical tuft, to...
Antithrombotic surfaces that prevent coagulation activation without interfering with haemostasis are required for blood-contacting devices. Such materials would restrain device-induced thrombogenesis and decrease the need anticoagulant use, thereby reducing unwanted bleeding. Here, by optimizing interactions factor XII rather than preventing its surface adsorption, we develop a substrate-independent antithrombotic polymeric coating sheltered positive charges. The antithrombic properties of...
During vertebrate retinal development, subsets of progenitor cells generate progeny in a non-stochastic manner, suggesting that these decisions are tightly regulated. However, the gene-regulatory network components functionally important largely unknown. Here we identify functional role for OTX2 transcription factor this process. CRISPR/Cas9 gene editing was used to produce somatic mutations chick retina and identified similar phenotypes those observed human patients. Single cell RNA...
Abstract Learning and memory are fundamental to adaptive behavior cognition. Various forms of synaptic plasticity have been proposed as cellular substrates for the emergence feature selectivity in neurons underlying episodic memory. However, despite decades work, our understanding how underlies encoding remains limited, largely due a shortage tools technical challenges associated with visualization at single-neuron resolution awake-behaving animals. Behavioral Timescale Synaptic Plasticity...
In presynaptic nerve terminals, the endocytic apparatus rapidly restores synaptic vesicles after neurotransmitter release. Many proteins localize to periactive zone, a loosely defined area adjacent active zones. A prevailing model posits that recruitment of these zone is activity-dependent. We here show targeting largely independent machinery and activity. At zones mouse hippocampal synapses Drosophila neuromuscular junctions, pharmacological or genetic silencing resulted in unchanged...
Fast electrical signaling in dendrites is central to neural computations that support adaptive behaviors. Conventional techniques lack temporal and spatial resolution the ability track underlying membrane potential dynamics present across complex three-dimensional dendritic arbor vivo. Here, we perform fast two-photon imaging of somatic single pyramidal cells CA1 region mouse hippocampus during awake behavior. We study subthreshold suprathreshold events throughout vivo by combining voltage...
The translocation of CCTα, the rate-limiting enzyme for phosphatidylcholine synthesis, to nuclear membranes and lipid droplets results in reversible dephosphorylation S319 sustained phosphorylation Y359+S362. Independent regulation these phosphosites P-domain CCTα is required activation on membranes.
Achievement of a stable surface coating with long-term resistance to biofilm formation remains challenge. Catechol-based polymerization chemistry and deposition are used as tools for modification diverse materials. However, the control coating, coverage, properties, protection against remain be solved. We report new approach based on supramolecular assembly generate long-acting antibiofilm coating. Here, we utilized catechol in combination low molecular weight amphiphilic polymers generation...
ABSTRACT Neuronal mitochondria play important roles beyond ATP generation, including Ca 2+ uptake, and therefore have instructive in synaptic function neuronal response properties. Mitochondrial morphology differs significantly the axon dendrites of a given subtype, but CA1 pyramidal neurons (PNs) hippocampus, within dendritic arbor also display remarkable degree subcellular, layer- specific compartmentalization. In these neurons, ranges from highly fused elongated apical tuft, to more...
Hippocampal place cells form a physiological substrate for spatial navigation and memory in the brain. Remarkably, CA1 pyramidal neurons can acquire stable fields few trials through novel behavioral timescale" synaptic plasticity rule (BTSP). While this process enable rapid learning, local feedback circuits likely tightly restrict recruitment of individual into ensemble representations; interaction between circuit dynamics BTSP remains unexplored. Here we developed \all-optical" approaches...
Fast electrical signaling in dendrites is central to neural computations that support adaptive behaviors. Conventional techniques lack temporal and spatial resolution the ability track underlying membrane potential dynamics present across complex three-dimensional dendritic arbor vivo . Here, we perform fast two-photon imaging of somatic single pyramidal cells CA1 region mouse hippocampus during awake behavior. We study subthreshold suprathreshold events throughout by combining voltage with...
Abstract Development of the vertebrate eye, like many developmental systems, depends on genes that are used iteratively in multiple distinct processes. The OTX2 transcription factor is one such gene, with a requirement for eye formation, photoreceptor and retinal pigment epithelium specification, among others. Recent evidence has suggested also expressed subsets progenitor cells restricted fate choices. However, given roles limitations conventional conditional knockout strategies, functional...
Abstract During retinal development, multipotent and restricted progenitor cells generate all of the neuronal retina. Among these are horizontal cells, which interneurons that modulate light-induced signal from photoreceptors. This study utilizes identification novel cis-regulatory elements as a method to examine gene regulatory networks direct development cells. Here we describe screen for elements, or enhancers, cell-associated genes PTF1A, ONECUT1 (OC1), TFAP2A (AP2A), LHX1. The OC1ECR22...
During retinal development, multipotent and restricted progenitor cells generate all of the neuronal retina. Among these are horizontal cells, which interneurons that modulate light-induced signal from photoreceptors. This study utilizes identification novel cis-regulatory elements as a method to examine gene regulatory networks direct development cells. Here we describe screen for elements, or enhancers, cell-associated genes PTF1A, ONECUT1 (OC1), TFAP2A (AP2A), LHX1. The OC1ECR22...
Abstract Introduction We aim to determine if white matter hyperintensities and decreased brain volumes are associated with sleep-disordered breathing (SDB), in a diverse sample of middle-aged older Hispanic/Latino adults. Methods Our 1,119 Hispanics/Latinos (ages than 50-years; 70% female) from the Hispanic Community Health Study/Study Latinos (HCHS/SOL) underwent magnetic resonance imaging (MRI) as part Study - Investigation Neurocognitive Aging MRI (SOL-INCA MRI) ancillary study. outcomes...