A5016424274- Blood Coagulation and Thrombosis Mechanisms
- Hemoglobinopathies and Related Disorders
- Hemophilia Treatment and Research
- Iron Metabolism and Disorders
- Blood donation and transfusion practices
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Platelet Disorders and Treatments
- Venous Thromboembolism Diagnosis and Management
- Protease and Inhibitor Mechanisms
- Blood transfusion and management
- Erythropoietin and Anemia Treatment
- Blood groups and transfusion
- Blood properties and coagulation
- Heparin-Induced Thrombocytopenia and Thrombosis
- Cell Adhesion Molecules Research
- Restless Legs Syndrome Research
- Erythrocyte Function and Pathophysiology
- Antiplatelet Therapy and Cardiovascular Diseases
- Atrial Fibrillation Management and Outcomes
- COVID-19 Clinical Research Studies
- Pregnancy and preeclampsia studies
- Cardiac and Coronary Surgery Techniques
- Therapeutic Uses of Natural Elements
- Peptidase Inhibition and Analysis
- Cancer-related gene regulation
Versiti Blood Center of Wisconsin
2015-2024
Medical College of Wisconsin
2014-2023
Institute of Cell Biology and Neurobiology
2019
Curtin University
2017
Mahidol University
2017
Seattle University
2016
Hudson Institute
2016
John Wiley & Sons (United States)
2016
Iowa City Public Library
2016
Westat (United States)
2010-2013
Soluble transferrin receptor (sTfR) and ferritin concentrations were measured in a variety of clinical settings to compare the ability these two tests identify iron deficiency. Among 62 anemic patients who either had bone marrow aspirate performed or documented response therapy, diagnostic sensitivity specificity sTfR (at cutoff > 2.8 mg/L) 92% 84%, respectively, with positive predictive value 42% this population. Ferritin (< = 12 microg/L) 25% 98%. However, could be improved 98%, by using <...
Neither the disease mechanism nor treatments for COVID-19 are currently known. Here, we present a novel molecular that provides therapeutic intervention points can be addressed with existing FDA-approved pharmaceuticals. The entry point virus is ACE2, which component of counteracting hypotensive axis RAS. Bradykinin potent part vasopressor system induces hypotension and vasodilation degraded by ACE enhanced angiotensin1-9 produced ACE2. perform new analysis on gene expression data from cells...
Key Points Genetic and biological variability in blood donors may impact RBC predisposition to hemolysis during cold storage after transfusion. Male sex, Asian or African American racial background, older age (>45 years) are significant modifiers of hemolysis.
ADVERTISEMENT RETURN TO ISSUEPREVArticleAnalysis of the plasma elimination kinetics and conformational stabilities native, proteinase-complexed reactive site cleaved serpins: comparison .alpha.1-proteinase inhibitor, .alpha.1-antichymotrypsin, antithrombin III, .alpha.2-antiplasmin, angiotensinogen, ovalbuminAlan E. Mast, Jan J. Enghild, Salvatore V. Pizzo, Guy SalvesenCite this: Biochemistry 1991, 30, 6, 1723–1730Publication Date (Print):February 12, 1991Publication History Published...
Blood donors are at risk of iron deficiency. We evaluated the effects blood donation intensity on and hemoglobin (Hb) in a prospective study.Four cohorts frequent first-time or reactivated (FT/RA) (no 2 years), female male, totaling 2425, were characterized followed as they donated frequently. At enrollment final visit, ferritin, soluble transferrin receptor (sTfR), Hb determined. Models to predict deficiency deferral developed. Iron depletion was defined two levels: erythropoiesis (IDE)...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTConformation of the reactive site loop .alpha.1-proteinase inhibitor probed by limited proteolysisAlan E. Mast, Jan J. Enghild, and Guy SalvesenCite this: Biochemistry 1992, 31, 10, 2720–2728Publication Date (Print):March 17, 1992Publication History Published online1 May 2002Published inissue 17 March 1992https://pubs.acs.org/doi/10.1021/bi00125a012https://doi.org/10.1021/bi00125a012research-articleACS PublicationsRequest reuse permissionsArticle...
BACKGROUND: Regular blood donors are at risk of iron deficiency, but characteristics that predispose to this condition poorly defined. STUDY DESIGN AND METHODS: A total 2425 red cell donors, either first‐time (FT) or reactivated (no donations for 2 years) frequent were recruited follow‐up. At enrollment, ferritin, soluble transferrin receptor (sTfR), and hemoglobin determined. Donor variables included demographics, smoking, dietary intake, use supplements, menstrual and/or pregnancy history....
Significance The generation of thrombin by prothrombinase, a complex composed activated (a) factors X (FXa) and V (FVa), is final step in blood coagulation. We demonstrate that tissue factor pathway inhibitor (TFPI) blocks prothrombinase at physiologically relevant rates concentrations, but only during the initiation clot formation. TFPI mediates this inhibitory activity through two high-affinity interactions, one with FXa FVa. This first description an endogenous human protein inhibits...
Although blood donation is allowed every 8 weeks in the United States, recovery of hemoglobin to currently accepted standard (12.5 g/dL) frequently delayed, and some donors become anemic.
Background. The evolutionary pressure of endemic malaria and other erythrocytic pathogens has shaped variation in genes encoding erythrocyte structural functional proteins, influencing responses to hemolytic stress during transfusion disease.
Extensive fibrin deposition in the lungs and altered levels of circulating blood coagulation proteins COVID-19 patients imply local derangement pathways that limit formation and/or promote its clearance. We examined transcriptional profiles bronchoalveolar lavage fluid (BALF) samples to identify molecular mechanisms underlying these coagulopathies. mRNA for regulators kallikrein–kinin (C1-inhibitor), (thrombomodulin, endothelial protein C receptor), fibrinolytic (urokinase urokinase...
Under normal conditions, reticulocytes are the youngest erythrocytes released from bone marrow into circulating blood. They mature for 1-3 days within and circulate 1-2 before becoming erythrocytes. Measurement of cellular hemoglobin concentration has long been reported by automated hematology analyzers as one red blood cell indices. The reticulocyte content (CHr or Ret-He) provides an indirect measure functional iron available new production over previous 3-4 days. in peripheral samples is...
Most known organisms encode proteases that are crucial for constitutive proteolytic events. In the present paper, we describe a method to define these events in proteomes from Escherichia coli humans. The takes advantage of specific N-terminal biotinylation protein samples, followed by affinity enrichment and conventional LC (liquid chromatography)-MS/MS (tandem mass spectrometry) analysis. is simple, uses easily obtainable reagents, applicable most proteomics facilities. As proof principle,...
Serpins encompass a superfamily of proteinase inhibitors that regulate many the serine proteinases involved in inflammation and hemostasis. In vitro, serpins are catalytically inactivated by they do not inhibit, leading to concept proteolytic down-regulation serpin inhibitory capacity. The extent which activity occurs vivo is debated, since little known rates at process occurs. To address this debate, we have measured inactivation three serpins, alpha 1-proteinase inhibitor (alpha 1PI),...
BACKGROUND Understanding the effect of blood donation and iron supplementation on balance will inform strategies to manage donor status. STUDY DESIGN AND METHODS A total 215 donors were randomized receive ferrous gluconate daily (37.5 mg iron) or no for 24 weeks after donation. Iron stores assessed using ferritin soluble transferrin receptor. Hemoglobin (Hb) was calculated from body Hb. Total (TBI) estimated by summing Hb iron. RESULTS At weeks, TBI in taking increased 281.0 (95% confidence...
BACKGROUNDRBC transfusion effectiveness varies due to donor, component, and recipient factors. Prior studies identified characteristics associated with variation in hemoglobin increments following transfusion. We extended these observations, examining donor genetic nongenetic factors affecting effectiveness.METHODSThis is a multicenter retrospective study of 46,705 patients 102,043 evaluable RBC transfusions from 2013 2016 across 12 hospitals. Transfusion was defined as hemoglobin,...