A5089884487- Neuroscience and Neuropharmacology Research
- Neuropeptides and Animal Physiology
- Neurobiology and Insect Physiology Research
- Pain Mechanisms and Treatments
- Photochromic and Fluorescence Chemistry
- Proteoglycans and glycosaminoglycans research
- Neurotransmitter Receptor Influence on Behavior
- Epilepsy research and treatment
- Photoreceptor and optogenetics research
- Parkinson's Disease Mechanisms and Treatments
- Receptor Mechanisms and Signaling
- Stress Responses and Cortisol
- Animal testing and alternatives
- Cerebral Palsy and Movement Disorders
- Ion Transport and Channel Regulation
- Mitochondrial Function and Pathology
- Lysosomal Storage Disorders Research
- Neuroendocrine regulation and behavior
- Neuroinflammation and Neurodegeneration Mechanisms
- Memory and Neural Mechanisms
- Ion channel regulation and function
- High Altitude and Hypoxia
- Tryptophan and brain disorders
- Pain Management and Opioid Use
- Neurogenesis and neuroplasticity mechanisms
Istituto Neurologico Mediterraneo
2017-2025
Sapienza University of Rome
2018-2019
Federico II University Hospital
2019
Abstract We moved from the hypothesis that perineuronal nets (PNNs), which are condensed structures of extracellular matrix surrounding GABAergic interneurons in forebrain, contribute to mechanisms maladaptive neuronal plasticity underlying chronic pain. Here, we found density PNNs labelled with lectin Wisteria Floribunda Agglutinin (WFA) increased contralateral somatosensory cortex (SSC), medial prefrontal (mPFC), reticular thalamic nucleus (RTN), and insular mice developing neuropathic...
Chronic pain is sustained by a maladaptive form of neuronal plasticity occurring in all stations the neuraxis, including cortical regions matrix. We report that chronic inflammatory induced unilateral injection complete Freund's adjuvant (CFA) hindpaw male mice was associated with progressive build-up perineuronal nets (PNNs) contralateral somatosensory cortex (SSC), medial prefrontal (mPFC), and reticular thalamic nucleus. In SSC, density PNNs labeled Wisteria floribunda agglutinin (WFA)...
Metabotropic glutamate (mGlu) receptors are candidate drug targets for therapeutic intervention in Parkinson's disease (PD). Here we focused on mGlu3, a receptor subtype involved synaptic regulation and neuroinflammation. mGlu3−/− mice showed an enhanced nigro-striatal damage microglial activation response to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Expression of genes encoding anti-inflammatory proteins neuroprotective factors was reduced the striatum MPTP-treated mice. We also...
BackgroundL-acetylcarnitine, a drug marketed for the treatment of chronic pain, causes analgesia by epigenetically up-regulating type-2 metabotropic glutamate (mGlu2) receptors in spinal cord. Because epigenetic mechanisms are typically long-lasting, we hypothesized that could outlast duration L-acetylcarnitine models inflammatory and neuropathic pain. ResultsA seven-day with (100 mg/kg, once day, i.p.) produced an antiallodynic effect complete Freund adjuvant mouse model...
Abstract Background and Hypothesis Type-1 trace amine-associated receptors (TAAR1) modulate dopaminergic glutamatergic neurotransmission are targeted by novel antipsychotic drugs. We hypothesized that schizophrenia (SCZ) causes adaptive changes in TAAR1 expression the prefrontal cortex. Study Design measured mRNA protein levels quantitative PCR immunoblotting post-mortem cortical samples obtained from 23 individuals affected SCZ non-schizophrenic controls (CTRL). Data were correlated with a...
Abstract Knowing the site of drug action is important to optimize effectiveness and address any side effects. We used light-sensitive drugs identify brain region-specific role mGlu5 metabotropic glutamate receptors in control pain. Optical activation systemic JF-NP-26, a caged, normally inactive, negative allosteric modulator (NAM) receptors, cingulate, prelimbic infralimbic cortices thalamus inhibited neuropathic pain hypersensitivity. Systemic treatment alloswitch-1, an intrinsically...
Acid-sensing ion channels (ASICs) are proton-gated involved in multiple biological functions such as: pain modulation, mechanosensation, neurotransmission, and neurodegeneration. Earlier, we described the genetic association, within Nuoro population, between Multiple Sclerosis (MS) rs28936, located ASIC2 3'UTR. Here investigated potential involvement of MS inflammatory process. We induced experimental autoimmune encephalomyelitis (EAE) wild-type (WT), knockout Asic1-/- Asic2-/- mice observed...
Previous studies suggest that different metabotropic glutamate (mGlu) receptor subtypes are potential drug targets for treating absence epilepsy. However, no information is available on mGlu3 receptors.To examine whether (i) changes of expression/signaling found in the somatosensory cortex and thalamus WAG/Rij rats developing spontaneous seizures; (ii) selective activation receptors with LY2794193 affects number duration spikewave discharges (SWDs) rats; (iii) a genetic variant GRM3...
Abstract mGlu5 metabotropic glutamate receptors are highly functional in the early postnatal life, and regulate developmental plasticity of parvalbumin-positive (PV + ) interneurons cerebral cortex. PV cells enwrapped by perineuronal nets (PNNs) at closure critical windows cortical plasticity. Changes PNNs have been associated with neurodevelopmental disorders. We found that number Wisteria Fluoribunda Agglutinin (WFA) density WFA /PV were largely increased somatosensory cortex −/− mice...
We used light-sensitive drugs to identify the brain region-specific role of mGlu5 metabotropic glutamate receptors in control pain. Optical activation systemic JF-NP-26, a caged, normally inactive, negative allosteric modulator (NAM) receptors, cingulate, prelimbic, and infralimbic cortices thalamus inhibited neuropathic pain hypersensitivity. Systemic treatment alloswitch-1, an intrinsically active receptor NAM, caused analgesia, effect was reversed by light-induced drug inactivation...
Thrombospondins, which are known to interact with the α2 δ subunit of voltage-sensitive calcium channels stimulate formation excitatory synapses, have recently been implicated in process epileptogenesis. No studies so far performed on thrombospondins models absence epilepsy. We examined whether expression gene encoding for thrombospondin-1 was altered brain WAG/Rij rats, model epilepsy humans. In addition, we frequency genetic variants THBS1 a large cohort children affected by...
Polymorphic variants of the gene encoding for metabotropic glutamate receptor 3 (mGlu3) are linked to schizophrenia. Because abnormalities cortical GABAergic interneurons lie at core pathophysiology schizophrenia, we examined whether mGlu3 receptors influence developmental trajectory transmission in postnatal life. ‐/‐ mice showed robust changes expression interneuron‐related genes prefrontal cortex (PFC), including large reductions parvalbumin (PV) and GluN1 subunit NMDA receptors. The...
Backgroud: Type-3 metabotropic glutamate (mGlu3) receptors are found in both neurons and glial cells regulate synaptic transmission, astrocyte function, microglial reactivity. Here we show that the genetic deletion of mGlu3 amplifies ischemic brain damage associated neuroinflammation adult mice. An increased infarct size was observed −/− mice CD1 C57Black strains 24 h following a permanent occlusion middle cerebral artery (MCA) as compared to their respective wild-type (mGlu3 +/+ mice)...
The epigenetic agents, L-acetylcarnitine (LAC) and L-methylfolate (MF) are putative candidates as add-on drugs in depression. We evaluated the effect of a combined treatment with LAC MF two different paradigms chronic stress mice human inducible pluripotent stem cells (hiPSCs) differentiated into dopaminergic neurons. Two groups were exposed to unpredictable (CUS) for 28 days or restraint (CRS) 21 day, (30 100 mg/kg) and/or (0.75 3 administered i.p. once day 14 days, starting from last week...
Abstract mGlu5 metabotropic glutamate receptors are highly expressed and functional in the early postnatal life, known to positively modulate NMDA receptor function. Here, we examined expression of subunits interneuron-related genes prefrontal cortex hippocampus −/− mice wild-type littermates at three developmental time points (PND9, − 21, 75). We were surprised find that all was greatly enhanced PND21. In contrast, PND9, GluN2B subunit enhanced, whereas GluN2A GluN2D reduced both regions....
Pilots and crew of domestic flights are exposed to transient periods mild reductions partial pressure inspired oxygen each day, this might have functional consequence on their performance in the long range. Here, we mice exposure (ROE) four times per day for 21 days by lowering levels corresponding an altitude about 2300 m, which is quote pressurization air cabin. Four groups were studied: unstressed or stressed ROE normoxic conditions. Mice chronic unpredictable stress (CUS) 28 days, was...