Weixiu Dong
A5052954420- Single-cell and spatial transcriptomics
- Neurogenesis and neuroplasticity mechanisms
- Bioinformatics and Genomic Networks
- Cell Image Analysis Techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Health, Environment, Cognitive Aging
- Congenital Diaphragmatic Hernia Studies
- Genetic Neurodegenerative Diseases
- Neural dynamics and brain function
- Neonatal Respiratory Health Research
- Radiomics and Machine Learning in Medical Imaging
- Atomic and Subatomic Physics Research
- Lung Cancer Diagnosis and Treatment
- Gene Regulatory Network Analysis
- Cancer-related molecular mechanisms research
- Genomics and Chromatin Dynamics
- Gene expression and cancer classification
- Genetic Associations and Epidemiology
Harvard University
2021-2024
University of California, San Diego
2020-2023
La Jolla Bioengineering Institute
2021
Abstract The primary motor cortex (M1) is essential for voluntary fine-motor control and functionally conserved across mammals 1 . Here, using high-throughput transcriptomic epigenomic profiling of more than 450,000 single nuclei in humans, marmoset monkeys mice, we demonstrate a broadly cellular makeup this region, with similarities that mirror evolutionary distance are consistent between the transcriptome epigenome. core molecular identities neuronal non-neuronal cell types allow us to...
Here we report the generation of a multimodal cell census and atlas mammalian primary motor cortex as initial product BRAIN Initiative Cell Census Network (BICCN). This was achieved by coordinated large-scale analyses single-cell transcriptomes, chromatin accessibility, DNA methylomes, spatially resolved morphological electrophysiological properties cellular resolution input-output mapping, integrated through cross-modal computational analysis. Our results advance collective knowledge...
Recent work has identified dozens of non-coding loci for Alzheimer's disease (AD) risk, but their mechanisms and AD transcriptional regulatory circuitry are poorly understood. Here, we profile epigenomic transcriptomic landscapes 850,000 nuclei from prefrontal cortexes 92 individuals with without to build a map the brain regulome, including profiles, regulators, co-accessibility modules, peak-to-gene links in cell-type-specific manner. We develop methods multimodal integration detecting...
Abstract The primary motor cortex (M1) is essential for voluntary fine control and functionally conserved across mammals. Using high-throughput transcriptomic epigenomic profiling of over 450,000 single nuclei in human, marmoset monkey, mouse, we demonstrate a broadly cellular makeup this region, whose similarity mirrors evolutionary distance consistent between the transcriptome epigenome. core molecular identity neuronal non-neuronal types allowed generation cross-species consensus cell...
To determine the impact of genetic variants on brain, we used genetically informed brain atlases in genome-wide association studies regional cortical surface area and thickness 39,898 adults 9136 children. We uncovered 440 significant loci discovery cohort 800 from a post hoc combined meta-analysis. Loci adulthood were largely captured childhood, showing signatures negative selection, linked to early neurodevelopment pathways associated with neuropsychiatric risk. Opposing gradations...
ABSTRACT We report the generation of a multimodal cell census and atlas mammalian primary motor cortex (MOp or M1) as initial product BRAIN Initiative Cell Census Network (BICCN). This was achieved by coordinated large-scale analyses single-cell transcriptomes, chromatin accessibility, DNA methylomes, spatially resolved morphological electrophysiological properties, cellular resolution input-output mapping, integrated through cross-modal computational analysis. Together, our results advance...
Ex-utero regulation of the lungs’ responses to breathing air and continued alveolar development shape adult respiratory health. Applying single-cell transposome hypersensitive site sequencing (scTHS-seq) over 80,000 cells, we assembled first regulatory atlas postnatal human mouse lung development. We defined modules elucidated new mechanistic insights directing septation, including type 1 myofibroblast cell signaling differentiation, a unique matrix fibroblast population. Incorporating GWAS,...
Abstract Human behaviors are at least partially driven by genomic regions that influence human-specific neurodevelopment. This includes undergoing human specific sequence acceleration (Human Accelerated Regions or HARs) and showing enhancer activity Gained Enhancers HGEs) not present in other primates. However, prior studies on HAR/HGE activities involved mixtures of brain cell types focused only putative downstream target genes. Here, we directly measured type the developing fetal using two...