A5047111729- Hemoglobinopathies and Related Disorders
- Erythrocyte Function and Pathophysiology
- Blood properties and coagulation
- Blood groups and transfusion
- Iron Metabolism and Disorders
- Blood transfusion and management
- Bone and Joint Diseases
- Spacecraft and Cryogenic Technologies
- Venous Thromboembolism Diagnosis and Management
- Biosensors and Analytical Detection
- SARS-CoV-2 detection and testing
- Biosimilars and Bioanalytical Methods
- Cyclone Separators and Fluid Dynamics
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Blood Coagulation and Thrombosis Mechanisms
- Advanced Biosensing Techniques and Applications
- Heavy Metal Exposure and Toxicity
- Hemoglobin structure and function
- Advanced biosensing and bioanalysis techniques
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Lattice Boltzmann Simulation Studies
- Polymer Surface Interaction Studies
- Aerosol Filtration and Electrostatic Precipitation
- Connexins and lens biology
- Microfluidic and Bio-sensing Technologies
Cleveland BioLabs (United States)
2021-2024
Case Western Reserve University
2015-2023
Case Engineering (United States)
2020
In-Q-Tel
2018
Coagulation factor XII (FXII) deficiency is associated with decreased neutrophil migration, but the mechanisms remain uncharacterized. Here, we examine how FXII contributes to inflammatory response. In 2 models of sterile inflammation, FXII-deficient mice (F12-/-) had fewer neutrophils recruited than WT mice. We discovered that produced a pool functionally distinct from hepatic-derived and trafficking at sites inflammation. signals in through urokinase plasminogen activator receptor-mediated...
Transfusion of red blood cells (RBCs) is one the most valuable and widespread treatments in modern medicine. Lifesaving RBC transfusions are facilitated by cold storage units banks worldwide. Currently, subsequent transfusion practices performed using simplistic workflows. More specifically, follow “first-in-first-out” principle to avoid wastage, whereas healthcare providers prefer “last-in-first-out” approach simply favoring chronologically younger RBCs. Neither addresses recent advances...
A gradient of microcapillary networks and microfluidic anastomoses enable standardized quantitative assessment red blood cell mediated microvascular occlusion.
Abstract Sickle cell disease (SCD) is a recessive genetic blood disorder exhibiting abnormal rheology. Polymerization of sickle hemoglobin, due to point mutation in the β‐globin gene results aberrantly adhesive and stiff red cells (RBCs). Hemolysis, RBC adhesion, rheology together impair endothelial health people with SCD, which leads cumulative systemic complications. Here, we describe microfluidic assay combined micro particle image velocimetry technique for integrated vitro assessment...
Summary Endothelial activation and sickle red blood cell (RBC) adhesion are central to the pathogenesis of disease (SCD). Quantitatively, RBC‐derived extracellular vesicles (REVs) more abundant from SS RBCs compared with healthy (AA RBCs). Sickle REVs (SS REVs) known promote endothelial (EC) through signalling transcriptional regulation at longer terms. However, REV‐mediated short‐term non‐transcriptional response EC is unclear. Here, we examined impact on acute microvascular RBC 2 h....
In sickle cell disease (SCD), 'disease severity' associates with increased RBC adhesion to quiescent endothelium, but the impact on activated endothelium is not known. Increased concentrations of free heme result from intravascular hemolysis in SCD. Heme essential for aerobic metabolism, and plays an important role numerous biological processes. Excess induces reactive oxygen species generation endothelial activation, which are associated cardiovascular disorders including atherosclerosis,...
This paper describes the design, fabrication, and testing of a microfluidic sensor for dielectric spectroscopy human whole blood during coagulation. The sensor, termed ClotChip, employs three-dimensional, parallel-plate, capacitive sensing structure with floating electrode integrated into channel. Interfaced an impedance analyzer, ClotChip measures complex relative permittivity, ε <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">r</sub> , in...
Abstract Sickle cell disease (SCD), which afflicts 100 000 Americans, as well millions worldwide, is associated with anemia, lifelong morbidity, and early mortality. Abnormal adhesion of sickle red blood cells (RBCs) to activated vascular endothelium may contribute acutely the initiation painful vaso-occlusive crises chronically endothelial damage in SCD. RBCs adhere through several mechanisms. In this study, using whole from 17 people heterozygous SCD (HbS variant) 55 homozygous (HbSS)...
Shear dependent adhesion of red blood cells is shown using a shear gradient microfluidic system that mimics human microvasculature.
A point-of-care diagnostic technology and approach is presented to perform both anemia detection hemoglobin variant identification in a single test using paper-based microchip electrophoresis.
We present a standardized in vitro microfluidic assay and Occlusion Index (OI) for the assessment of red blood cell (RBC)-mediated microcapillary occlusion its clinical associations sickle disease (SCD).Red mediated represented by OI were assessed seven subjects with hemoglobin-SC (HbSC), 18 homozygous SCD (HbSS), five control individuals (HbAA).We identified two sub-populations HbSS based on distribution. relatively higher OIs had significantly lower hemoglobin levels, fetal (HbF) mean...
Abstract Abnormal erythrocyte adhesion owing to polymerization of sickle hemoglobin is central the pathophysiology cell disease (SCD). Mature erythrocytes constitute &gt;80% all in SCD; however, relative contributions acute and chronic vasculopathy SCD are not well understood. Here, we showed that bending stress exerted on plasma membrane by under hypoxia, enhances sulfatide-mediated abnormal mature adhesion. We hypothesized sphingomyelinase (SMase) activity, which upregulated...
This article describes novel measurements of the velocity whole blood flow in a microchannel during coagulation. The is imaged volumetrically using simple optical setup involving white light source and microscope camera. images are processed particle image velocimetry (PIV) wavelet-based (wOFV), both which use individual cells as tracers. Measurements several clinically relevant parameters such clotting time, decay rate, blockage ratio computed. high-resolution wOFV results yield highly...
Red blood cell (RBC) deformability is a valuable hemorheological biomarker that can be used to assess the clinical status and response therapy of individuals with sickle disease (SCD). RBC has been measured by ektacytometry for decades, which uses shear or osmolar stress. However, population based measurement does not detect small-fractions abnormal RBCs. A single cell-based, functional assay would complement provide additional information. Here, we tested relative merits OcclusionChip,...
This paper describes the design and construct of a fully biocompatible, microfluidic, dielectric sensor targeted at monitoring human whole blood coagulation point-of-care (POC). The assembly procedure involves using sputtered electrodes in microfluidic channel with physiologically relevant height 50μm to create three-dimensional (3D), parallel-plate, capacitive sensing area. is constructed biocompatible materials polymethyl methacrylate (PMMA) for substrate titanium nitride (TiN) floating...
Neural activity that occur during motor movement, speech, thought, and various other events can be observed in the form of brainwaves composed synchronized electrical pulses emitted from adjoining communicative neurons. Observations these have been made possible through neurodevices, which detect changes and/or mechanical parameters. For decades, field neuroscience has enriched by utilization neurotechnologies at microscale, begun to gain further enhancement with introduction nanotechnology....