A5081152811- Erythrocyte Function and Pathophysiology
- Blood transfusion and management
- Blood groups and transfusion
- Blood donation and transfusion practices
- Blood properties and coagulation
- Reproductive Biology and Fertility
- Hemoglobinopathies and Related Disorders
- Freezing and Crystallization Processes
- Hemoglobin structure and function
- Neonatal Health and Biochemistry
- Physiological and biochemical adaptations
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Pancreatic function and diabetes
- Thermoregulation and physiological responses
- Spaceflight effects on biology
- 3D Printing in Biomedical Research
- Mesenchymal stem cell research
- Erythropoietin and Anemia Treatment
- Pluripotent Stem Cells Research
- Hematopoietic Stem Cell Transplantation
- nanoparticles nucleation surface interactions
- Extracellular vesicles in disease
- Digital Imaging for Blood Diseases
- Tissue Engineering and Regenerative Medicine
- Thermal Regulation in Medicine
University of Alberta
2016-2025
Canadian Blood Services
2016-2025
Seattle University
2018-2024
University of Ottawa
2015-2024
Royal North Shore Hospital
2024
The George Institute for Global Health
2024
Ottawa Hospital
2024
Université de Montréal
2024
University of Minnesota System
2023
Anna Needs Neuroblastoma Answers
2014-2023
Hemolysis of red blood cells (RBCs) during bank storage is the most obvious manifestation RBC system failure. However, its analysis made difficult because largest source interunit difference donor specific. Availability data from national systems on large numbers units used for internal quality control (QC) purposes and stored processed in uniform ways permits statistical analysis.Measures hemolysis at end randomly selected observed QC were obtained four systems. Groups these measures that...
Background During storage detrimental biochemical and biomechanical changes occur within a red blood cell ( RBC ). microparticles RMPs ) produced during have been identified as biomarkers of quality, being potentially immunogenic inhibitory to nitric oxide regulation. Study Design Methods In this study, microvesiculation in the composition membrane were investigated throughout 49 days correlated with vitro assays examining quality. Leukoreduced units using buffy coat method collected stored...
Red blood cell (RBC) hemolysis represents an intrinsic mechanism for human vascular disease. Intravascular releases hemoglobin and other metabolites that inhibit nitric oxide signaling drive oxidative inflammatory stress. Although these pathways are important in disease pathogenesis, genetic population modifiers of hemolysis, including sex, have not been established.We studied sex differences storage or stress-induced RBC units from the United States Canada 22 inbred mouse strains patients...
While red blood cells (RBCs) are administered to improve oxygen delivery and patient outcomes, they also have been associated with potential harm. Unlike solid organ transplantation, the clinical consequences of donor characteristics on recipients not evaluated in transfusion medicine.To analyze association RBC age sex survival recipients.We established a longitudinal cohort by linking data from collection agency administrative at 4 academic hospitals.Cox proportional hazards regression...
Abstract In North America, red blood cells (RBCs) are cryopreserved in a clinical setting using high glycerol concentrations (40% w/v) with slow cooling rates (~1°C/min) prior to storage at −80°C, while European protocols use reduced rapid freezing rates. After thawing and transfusion, must be removed avoid intravascular hemolysis. This is time consuming process requiring specialized equipment. Small molecule ice recrystallization inhibitors (IRIs) such as β-PMP-Glc β-pBrPh-Glc have the...
Canadian Blood Services has been conducting quality monitoring of red blood cell (RBC) components since 2005, a period spanning the implementation semiautomated component production. The aim was to compare RBC produced before and after this production method change.Data from 572 units were analyzed, categorized by method: Method 1, manual methods; 2, buffy coat method; 3, whole filtration method. assessed using an extensive panel in vitro tests, encompassing regulated control criteria such...
Background and Objectives Quality control ( QC ) data collected by blood services are used to monitor production ensure compliance with regulatory standards. We demonstrate how analysis of quality can be highlight the sources variability within red cell concentrates RCC s). Materials Methods merged Canadian Blood Services manufacturing donor records for 28 227 between June 2011 October 2014. Units were categorized based on processing method, bag manufacturer, age sex, then assessed product...
Stored red blood cells (RBCs) are needed for life-saving transfusions, but they undergo continuous degradation. RBC storage lesions often assessed by microscopic examination or biochemical and biophysical assays, which complex, time-consuming, destructive to fragile cells. Here we demonstrate the use of label-free imaging flow cytometry deep learning characterize lesions. Using brightfield images, a trained neural network achieved 76.7% agreement with experts in classifying seven clinically...
Transfusion of red blood cells (RBCs) is one the most valuable and widespread treatments in modern medicine. Lifesaving RBC transfusions are facilitated by cold storage units banks worldwide. Currently, subsequent transfusion practices performed using simplistic workflows. More specifically, follow “first-in-first-out” principle to avoid wastage, whereas healthcare providers prefer “last-in-first-out” approach simply favoring chronologically younger RBCs. Neither addresses recent advances...
BackgroundThe effect of a liberal red-cell transfusion strategy as compared with restrictive in patients during the critical care period after an aneurysmal subarachnoid hemorrhage is unclear.MethodsWe randomly assigned critically ill adults acute and anemia to (mandatory at hemoglobin level ≤10 g per deciliter) or (optional ≤8 deciliter). The primary outcome was unfavorable neurologic outcome, defined score 4 higher on modified Rankin scale (range, 0 6, scores indicating greater disability)...
Abstract Background Existing research presents conflicting results on the influence of blood donor sex hemoglobin (Hb) change and transfusion‐associated infection mortality in transfusion recipients. Aim This retrospective study explored association between recipient hospital‐onset sepsis (HO‐sepsis) Hb changes critically ill patients receiving red cell (RBC) transfusions. Methods Data from 2010–2020 were extracted an academic hospital's clinical database a supplier's database. HO‐sepsis was...
Historically, red cell concentrates (RCCs) have been manually glycerolized and deglycerolized using an open system (COBE 2991, Terumo). Implementation of a closed processor (ACP-215, Haemonetics) for glycerolization deglycerolization RCCs creates challenge management the historic cryopreserved RCC inventory. A study was undertaken to determine whether frozen could be processor, as processors are being discontinued. Thirteen ABO/Rh matched were pooled split produce six large (approximately...
Red blood cell (RBC) transfusion, a life-saving intervention, is limited by reduced RBC potency over time. Cold storage at + 4°C for up to 42 days can reduce transfusion efficacy due alterations termed the "storage lesion." Strategies mitigate lesion include alkaline additive solutions and supercooled extend reducing metabolic stresses. However, metabolism during in standard or additives remains unstudied. This study, thus, investigated impact of (alkaline E-Sol5 SAGM) temperatures (+ 4°C,...
While stem cell cryopreservation methods have been optimized using dimethylsulfoxide (DMSO), the established techniques are not optimal when applied to unfertilized human embryonic cells. In addition, important questions remain regarding toxicity and characteristics of DMSO for treatment cells clinical use. The objective this study was establish an method low concentrations (0.2 M) trehalose, a nontoxic disaccharide glucose, which possesses excellent protective characteristics, in place...
Abstract During cryopreservation, ice recrystallization is a major cause of cellular damage. Conventional cryoprotectants such as dimethyl sulfoxide (DMSO) and glycerol function by number different mechanisms but do not mitigate or control at concentrations utilized in cryopreservation procedures. In North America, human red blood cells (RBCs) utilizes high glycerol. RBC units frozen under these conditions must be subjected to time-consuming deglycerolization process after thawing order...
Background and Objectives While the clinical impact of differences in red blood cell ( RBC ) component processing methods is unknown, there are concerns they may be confounding variables studies such as ongoing ‘age blood’ investigations. Here, we compare vitro characteristics concentrates RCC s) produced by several different methods. Materials Methods Nine were examined: three apheresis (Alyx, MCS + Trima), well leucoreduced whole blood‐derived s buffy coat filtration non‐leucoreduced s....
Damage-associated molecular patterns (DAMPs) are found in transfusion products, but their potential impacts not fully understood. We examined the influence of manufacturing method on levels mitochondrial (mt) DNA and extracellular vesicle (EV) DAMPs red cell concentrates (RCCs).Eighty-seven RCCs were prepared using nine different methods (6-15 units/method), including three apheresis, five whole blood (WB)-derived leucoreduced (LR) one WB-derived non-LR method. On storage days 5 42, mtDNA...