A5027727824- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Vector-Borne Animal Diseases
- Mosquito-borne diseases and control
- Viral Infections and Immunology Research
- RNA and protein synthesis mechanisms
- Animal Disease Management and Epidemiology
- Poxvirus research and outbreaks
- Herpesvirus Infections and Treatments
- Respiratory viral infections research
- Animal Virus Infections Studies
- Environmental Conservation and Management
- Influenza Virus Research Studies
- Monoclonal and Polyclonal Antibodies Research
Wageningen University & Research
2010-2024
Utrecht University
2010-2020
Rift Valley fever virus (RVFV), an emerging arthropod-borne pathogen, has a broad host and cell tropism. Here we report that the glycosaminoglycan heparan sulfate, abundantly present on surface of most animal cells, is required for efficient entry RVFV. Entry was significantly reduced by preincubating inoculum with highly sulfated heparin, enzymatic removal sulfate from cells in genetically deficient synthesis.
The Rift Valley fever virus (RVFV) is transmitted by infected mosquitoes, causing severe disease in humans and livestock across Africa. We determined the x-ray structure of RVFV class II fusion protein Gc its postfusion form complex with a glycerophospholipid (GPL) bound conserved cavity next to loop. Site-directed mutagenesis molecular dynamics simulations further revealed built-in motif allowing en bloc insertion loop into membranes, making few nonpolar side-chain interactions aliphatic...
The entry of the enveloped Rift Valley fever virus (RVFV) into its host cell is mediated by viral glycoproteins Gn and Gc. We investigated RVFV process and, in particular, pH-dependent activation mechanism using our recently developed nonspreading-RVFV-particle system. Entry was efficiently inhibited lysosomotropic agents that prevent endosomal acidification compounds interfere with dynamin- clathrin-dependent endocytosis. Exposure plasma membrane-bound virions to an acidic pH (<pH 6)...
Enteroviruses (EVs) represent many important pathogens of humans. Unfortunately, no antiviral compounds currently exist to treat infections with these viruses. We screened the Prestwick Chemical Library, a library approved drugs, for inhibitors coxsackievirus B3, identified pirlindole as potent novel inhibitor, and confirmed inhibitory action dibucaine, zuclopenthixol, fluoxetine, formoterol. Upon testing viruses several EV species, we found that dibucaine inhibited EV-B EV-D also EV-A, but...
The ongoing threat of viral infections and the emergence antiviral drug resistance warrants a ceaseless search for new compounds. Broadly-inhibiting compounds that act on elements shared by many viruses are promising candidates. Here, we identify peptide derived from cowpox virus protein CPXV012 as broad-spectrum peptide. We found hampers infection multitude clinically economically important enveloped viruses, including poxviruses, herpes simplex virus-1, hepatitis B virus, HIV-1, Rift...
Abstract Background Next-generation sequencing (NGS) analysis was compared to the current MAPREC (mutational by polymerase chain reaction and restriction enzyme cleavage) assay for quality control of live-attenuated oral polio vaccine (OPV). Methods measures reversion main OPV attenuating mutations such as uracil (U) cytosine (C) at nucleotide 472 in 5′ noncoding region type 3 OPV. Eleven samples were analyzed 8 laboratories using their in-house NGS method. Results Intraassay,...