A5070237362- Bacterial Infections and Vaccines
- Bacterial Genetics and Biotechnology
- Amino Acid Enzymes and Metabolism
- Epigenetics and DNA Methylation
- Bacteriophages and microbial interactions
- Cancer, Hypoxia, and Metabolism
- Antimicrobial Peptides and Activities
- Metabolism and Genetic Disorders
- Autophagy in Disease and Therapy
- Plant Molecular Biology Research
- Animal Virus Infections Studies
- Plant Reproductive Biology
- Calcium signaling and nucleotide metabolism
- Hippo pathway signaling and YAP/TAZ
- Cancer Cells and Metastasis
- Polyamine Metabolism and Applications
- Erythrocyte Function and Pathophysiology
- FOXO transcription factor regulation
- Antibiotic Resistance in Bacteria
- Cellular transport and secretion
- Neuroblastoma Research and Treatments
- Hepatitis C virus research
- Pancreatic function and diabetes
- Plant tissue culture and regeneration
- HIV Research and Treatment
Universidad Publica de Navarra
2024
Goethe University Frankfurt
2023
Institute for Research in Biomedicine
2016-2022
Universitat de Barcelona
2016-2018
Nottingham University Hospitals NHS Trust
2016
NIHR Nottingham Digestive Diseases Biomedical Research Unit
2016
University of Nottingham
2016
Utrecht University
2014-2016
Centre for Biomedical Network Research on Rare Diseases
2016
CD98hc functions as an amino acid (AA) transporter (together with another subunit) and integrin signaling enhancer. It is overexpressed in highly proliferative cells both physiological pathological conditions. deletion induces strong impairment of cell proliferation vivo vitro. Here, we investigate CD98hc-associated AA transport survival proliferation. By using chimeric versions CD98hc, the two protein can be uncoupled. Although recovering capacity restores vitro CD98hc-null cells,...
Abstract CD98 heavy chain (CD98hc) forms heteromeric amino acid (AA) transporters by interacting with different light chains. Cancer cells overexpress CD98hc-transporters in order to meet their increased nutritional and antioxidant demands, since they provide branched-chain AA (BCAA) aromatic (AAA) availability while protecting from oxidative stress. Here we show that BCAA AAA shortage phenocopies the inhibition of mTORC1 signalling, protein synthesis cell proliferation caused CD98hc...
Autotransporters (ATs) are proteins secreted by Gram-negative bacteria that often play a role in virulence. Eight different ATs have been identified Neisseria meningitidis, but only six of them characterized. AutA is one the remaining ATs. Its expression remains controversial. Here, we show autA gene present many neisserial species, its disrupted various genetic features; however, it expressed certain strains N. meningitidis. By sequencing large panels disease isolates and Western blot...
Abstract Recent successes in developing small-molecule degraders that act through the ubiquitin system have spurred efforts to extend this technology other mechanisms, including autophagosomal-lysosomal pathway. Therefore, reports of autophagosome tethering compounds (ATTECs) received considerable attention from drug development community. ATTECs are based on target recruitment LC3/GABARAP, a family membrane-bound proteins tether autophagy receptors autophagosome. In order validate existing...
Neisseria meningitidis is a Gram-negative bacterium that resides as commensal in the upper respiratory tract of humans, but occasionally, it invades host and causes sepsis and/or meningitis. The can produce eight autotransporters, seven which have been studied to some detail. remaining one, AutB, has not characterized yet. Here, we show autB gene broadly distributed among pathogenic spp. intact most meningococcal strains. However, its expression prone phase variation due slipped-strand...
A novel and broadly applicable strategy combining site-directed mutagenesis DNA assembly for constructing seamless viral chimeras is described using hepatitis C virus (HCV) as an exemplar. Full-length HCV genomic cloning cassettes, which contained flexibly situated restriction endonuclease sites, were prepared via a single, reaction digested to receive PCR-amplified envelope genes by In-Fusion cloning. Using this method, we able construct gene-shuttle cassettes generation of cell...
One of the most important effects climate change in wine-growing areas is advance phenological stages, especially concerning early berry ripening. In hottest seasons, this results a lack synchrony between sugar and phenolic ripeness. order to cope with fact, general effort being made by researchers growers aiming at delaying ripening through different strategies. proposed approaches application elicitors. This study aims assess effect transcriptomic level three hormone- natural-based...