A5058354480- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Plant Pathogenic Bacteria Studies
- Phytoplasmas and Hemiptera pathogens
- Plant Disease Resistance and Genetics
- Cocoa and Sweet Potato Agronomy
- Insect-Plant Interactions and Control
- Herpesvirus Infections and Treatments
- Research on scale insects
- Insect and Pesticide Research
- Insect symbiosis and bacterial influences
- Viral Infections and Immunology Research
- Poxvirus research and outbreaks
- Studies on Chitinases and Chitosanases
Canadian Food Inspection Agency
2013-2023
Plum pox virus (PPV), a member of the genus Potyvirus, family Potyviridae, is positive-sense single-stranded RNA virus. viruses tend to be genetically diverse because viral RNA-dependent polymerase responsible for replication lacks proofreading capacity. Consequently, PPV diverse, and nine strains (D, M, EA, C, Rec, W, T, CR, An) have been identified date. At least two these (Rec, T) are direct products recombination events, contributor genetic diversity common occurrence in Potyvirus. The...
Ten isolates of apple hammerhead viroid-like RNA (AHVd RNA) were detected by next-generation sequencing (NGS) and/or RT-PCR analysis (Malus domestica) 'Pacific Gala' (PG) from Canada. All consisted 433 nucleotides in size, and their circular structure sequence supported reverse transcription amplification with two separate pairs abutted primers, cloning sequencing. Two regions defined for the computer-predicted secondary AHVd RNA-PG – a region (HHR) loop-rich (LRR). The HHR contained core...
Phytoplasmas are insect-vectored, difficult-to-culture bacterial pathogens that infect a wide variety of crop and non-crop plants, associated with diseases can lead to significant yield losses in agricultural production worldwide. currently grouped the provisional genus 'Candidatus Phytoplasma', which includes 49 'Candidatus' species. Further differentiation phytoplasmas into ribosomal groups is based on restriction fragment length polymorphism (RFLP) pattern 16S rRNA-encoding operon, more...
Plum pox virus (PPV) displays significant genetic diversity that has resulted in the identification of several different strains, some differ their biological properties and geographic distribution. Some strains are limited distribution to a single country, while isolates PPV strain D found every country where been detected. Many questions remain regarding evolutionary history PPV: its (strain) diversification, origin, dispersal and/or strains. To explore evolution PPV, inferred ancestral...
Blackcurrant leaf chlorosis associated virus (BCLCaV) was detected recently by next-generation sequencing (NGS) and a new distinct species in the genus Idaeovirus proposed. Analysis of NGS-derived paired-end reads revealed existence bridge encompassing 3′-terminus 5′-terminus RNA-2 or RNA-3 BCLCaV. The full could be amplified using outward facing abutting primers; also, RNA-2/RNA-3 even after three consecutive RNase R enzyme treatments, with denaturation at 95 °C preceding each digestion....
Rubus stunt, caused by 'Candidatus Phytoplasma rubi' (Rubus stunt phytoplasma; RSP), is an economically important disease of Rubus. This occurs in wild and cultivated spp. Europe but has not been reported from North America; however, its major leafhopper vector well established western Canada the U.S.A. RSP potential to impact cane-fruit industry significantly compromising yields impacting export for propagation material. To mitigate risk this entering or establishing, import testing...
A dataset of approximately 85 apple hammerhead viroid (AHVd) genome sequences was analysed to determine genetic diversity. Sequences included 18 new AHVd variants detected in a 2017/2018 survey, as well suitable deposited GenBank. Two phylogenetically distinct clades were observed. range indel events identified with most ranging size from 433 435 nt. None these appear affect the predicted secondary structure AHVd, which supported by prevalence unambiguous covariation and or substitutions...
High throughput sequencing (HTS) revealed the existence of complexes fabaviruses infecting two 'Staccato(R) ' sweet cherry plants (Prunus avium). Species classified as members genus Fabavirus are positive sense RNA viruses that possess bipartite genomes. The Stac-3B (symptomatic) and Stac-4B (asymptomatic) were each determined to be infected with multiple RNA1 RNA2 segments Prunus virus F (PrVF) putative (CVF). Stac-3B_C3 Stac-3B_c11, respectively, proposed represent an isolate identified...
Partial molecular characterization of isolates Plum pox virus (PPV) from Kazakhstan was described by Spiegel et al. (2004). The were classified as members the Dideron (D) strain based on: a) RFLP analysis a 243-bp amplicon in region C terminus coat protein (CP) fragment, b) an 836-bp P3-6K1 coding and c) CP region. plum detected trees maintained selection-orchard Pomological Garden, Almaty, Kazakhstan. apricot wild seedlings obtained originally Zailiyski Alatou at northern edge Tien-Shan...
Blackcurrant leaf chlorosis associated virus (BCLCaV) was detected recently by next-generation sequencing (NGS) and proposed as a new distinct species in the genus Idaeovirus. Genomic components of BCLCaV that were confirmed include: 1) RNA-1 is monocistronic encodes replicase complex; 2) bicistronic RNA-2 movement protein (MP) coat (CP) virus, with open reading frames (ORF) overlap single adenine (A) nucleotide (nt) representing third position an opal stop codon MP ORF2a first start CP...