A5002030546- Polysaccharides and Plant Cell Walls
- Plant Molecular Biology Research
- Polysaccharides Composition and Applications
- Advanced Cellulose Research Studies
- Plant nutrient uptake and metabolism
- Tree Root and Stability Studies
- Natural Fiber Reinforced Composites
- Food composition and properties
- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Soybean genetics and cultivation
- Soil Management and Crop Yield
- Arctic and Russian Policy Studies
- Proteoglycans and glycosaminoglycans research
- Magnetic and Electromagnetic Effects
- Structural Analysis and Optimization
- Growth and nutrition in plants
- Biocrusts and Microbial Ecology
- Plant responses to water stress
- Lignin and Wood Chemistry
- Soil Mechanics and Vehicle Dynamics
- Plant and Biological Electrophysiology Studies
- Plant tissue culture and regeneration
Kazan Institute of Biochemistry and Biophysics
2007-2025
Russian Academy of Sciences
2010-2022
Karelian Research Centre
2022
Abstract The intrusive growth, a type of plant cell elongation occurring in the depths tissues, is characterized by invasion growing between its neighbours due to higher rate elongation. In order reveal largely unknown molecular mechanisms we isolated primary flax phloem fibers specifically at stage growth laser microdissection. comparison RNA-Seq data from several stem parts enabled characterization those processes during fiber revealed players are summarized as involved supply assimilates...
Contractile cell walls are found in various plant organs and tissues such as tendrils, contractile roots, tension wood. The tension-generating mechanism is not known but thought to involve special wall architecture. We previously postulated that could result from the entrapment of certain matrix polymers within cellulose microfibrils. As reported here, this hypothesis was corroborated by sequential extraction analysis retained microfibrils wood normal hybrid aspen (Populus tremula × Populus...
Plant fibres—cells with important mechanical functions and a widely used raw material—are usually identified in microscopic sections only after reaching significant length or developing thickened cell wall. We characterized the early developmental stages of hemp (Cannabis sativa) stem phloem fibres, both primary (originating from procambium) secondary cambium), when they still had gave major emphasis to role intrusive elongation, specific type plant growth by which fibres commonly attain...
Biomechanical parameters of plant tissues and organs are increasingly recognized as key factors in development application, increasing the demand for convenient devices their study. The paper presents an original device performing a three-point bending test using inverse method, which is modification classical (straight) test. designed was tested experiments to determine modulus elasticity flax stems, results were compared with data obtained vibration method straight on commercial...
Plant fibers find wide application in various fields that demand specific parameters of fiber quality. To develop approaches for the improvement yield and quality bast fibers, knowledge developmental stages key molecular players are responsible a certain parameter, vitally important. In present review development, such as initiation, intrusive growth, formation thickened cell wall layers (secondary tertiary walls) considered, well impact each these on final The problems perspectives crop...
Cellulose synthesising complex consists of cellulose synthase (CESA) subunits encoded by a multigene family; different sets CESA genes are known to be expressed during primary and secondary cell wall formation. We examined the expression LusCESAs in flax (Linum usitatissimum L.) cellulosic fibres at various stages development course graviresponse means RNA-Seq quantitative PCR. Transcripts for both wall-related CESAs were abundant depositing highly tertiary walls. Gravistimulation plants...
Phloem fibers are important elements of plant architecture and the target product many fiber crops. A key stage in development is intrusive elongation, mechanisms which largely unknown. Integrated analysis miRNA mRNA expression profiles intrusivelygrowing obtained by laser microdissection from flax (Linum usitatissimum L.) stem revealed all 124 known 23 gene families potential targets differentially expressed miRNAs. comparison between phloem at different developmental stages, parenchyma...
Cell wall thickening and development of secondary cell walls was a major step in plant terrestrialization that provided the mechanical support, effective functioning water-conducting elements fortification surface tissues. Despite its importance, diversity, emergence evolution early land plants have been characterized quite poorly. Secondary can be present different types with fibers being among ones. The necessity for support upon increasing height is widely recognized; however,...
The cellulose-enriched tertiary cell walls present in many plant fibers have specific composition, architecture, machinery of formation, and function. To better understand the mechanisms underlying their mode action to reveal peculiarities from different species, it is necessary more deeply characterize major components. Next overwhelming cellulose, rhamnogalacturonan I (RG-I) considered be key polymer wall; however, has been isolated biochemically characterized very few species. Here, we...
Examples of labeling polysaccharides at hydroxyl groups are described in this paper, which especially demand for molecules with a blocked reducing end. The protocols presented suitable the microscale synthesis labeled that do not require chromatography step isolation. include (1) direct modification fluorescein isothiocyanate; (2) reaction fluorescein-dichlorotriazine derivative; (3) biotin-dichlorotriazine; (4) indirect two-step (given glycosphingolipid) glutaric anhydride followed by...