A5017813247- Plant Reproductive Biology
- Polysaccharides and Plant Cell Walls
- Plant Molecular Biology Research
- Biofuel production and bioconversion
- Plant Parasitism and Resistance
- Plant nutrient uptake and metabolism
- Plant-Microbe Interactions and Immunity
- Mycorrhizal Fungi and Plant Interactions
- Advanced Cellulose Research Studies
- Polysaccharides Composition and Applications
- Plant Gene Expression Analysis
- Agriculture, Plant Science, Crop Management
- Greenhouse Technology and Climate Control
- Plant tissue culture and regeneration
- Plant Physiology and Cultivation Studies
- Plant Diversity and Evolution
- Lichen and fungal ecology
- Lipid metabolism and biosynthesis
- Light effects on plants
- Tree Root and Stability Studies
- Botany and Plant Ecology Studies
- Molecular Spectroscopy and Structure
- Plant and animal studies
- Postharvest Quality and Shelf Life Management
- Soybean genetics and cultivation
University of Wrocław
2014-2024
Umeå Plant Science Centre
2007-2021
Swedish University of Agricultural Sciences
2015-2021
Petersburg Nuclear Physics Institute
2009
KTH Royal Institute of Technology
2009
AlbaNova
2009
Tension wood is a specialized tissue of deciduous trees that functions in bending woody stems to optimize their position space. fibers develop on one side the stem have an increased potency shrink compared with opposite side, thus creating moment. It believed gelatinous (G) cell wall layer containing almost pure cellulose tension pivotal shrinking. By analyzing saccharide composition and linkage isolated G-layers poplar, we found they contain some matrix components addition cellulose, which...
KORRIGAN1 (KOR1) is a membrane-bound cellulase implicated in cellulose biosynthesis. PttCel9A1 from hybrid aspen (Populus tremula L. × tremuloides Michx.) has high sequence similarity to KOR1 and we demonstrate here that it complements kor1-1 mutants, indicating ortholog. We investigated the function of PttCel9A1/KOR1 Arabidopsis secondary growth using transgenic lines expressing 35S::PttCel9A1 mutant line irx2-2. The presence elevated levels walls was confirmed by muro visualization...
Contractile cell walls are found in various plant organs and tissues such as tendrils, contractile roots, tension wood. The tension-generating mechanism is not known but thought to involve special wall architecture. We previously postulated that could result from the entrapment of certain matrix polymers within cellulose microfibrils. As reported here, this hypothesis was corroborated by sequential extraction analysis retained microfibrils wood normal hybrid aspen (Populus tremula × Populus...
Summary Cell wall hemicelluloses and pectins are O‐ acetylated at specific positions, but the significance of these substitutions is poorly understood. Using a transgenic approach, we investigated how reducing extent O ‐acetylation in xylan affects cell chemistry, plant performance recalcitrance lignocellulose to saccharification. The Aspergillus niger acetyl esterase An AXE 1 was expressed Arabidopsis under control either constitutively 35S CAMV promoter or woody‐tissue‐specific GT 43B...
Xyloglucan is the major hemicellulose of dicotyledon primary cell walls, affecting load-bearing framework with participation xyloglucan
Regularity and periodicity in the arrangements of organs all groups land plants raise questions about mechanisms underlying phyllotactic pattern formation. The initiation lateral (leaves, flowers, etc.), thus, their spatio-temporal positioning, occurs shoot apical meristem (SAM) is related to structure organogenic activity meristem. In this review, we present some aspects diversity stability patterns major lineages plants, from bryophytes angiosperms, which SAM structures differ...
Abstract Auxin is a small molecule involved in most processes related to plant growth and development. Its effect usually depends on the distribution tissues formation of concentration gradients. Until now there has been no tool for direct tracking auxin transport at cellular tissue level; therefore majority studies have based various indirect methods. However, due their restrictions, relatively little known about relationship between pathways specific developmental processes. We present new...
Summary Xylem fibers are highly elongated cells that key constituents of wood, play major physiological roles in plants, comprise an important terrestrial carbon reservoir, and thus have enormous ecological economic importance. As they develop, from fusiform initials, their bodies remain the same length while tips elongate intrude into intercellular spaces. To elucidate mechanisms tip elongation, we studied cell wall along isolated, elongating aspen xylem used computer simulations to predict...
There currently exists a diverse array of molecular probes for the in situ localization polysaccharides, nucleic acids, and proteins plant cells, including reporter enzyme strategies (e.g. protein-glucuronidase fusions). In contrast, however, there is paucity methods direct analysis endogenous glycoside hydrolases transglycosidases responsible cell wall remodeling. To exemplify potential fluorogenic resorufin glycosides to address this issue, β-glycoside xylogluco-oligosaccharide...
Axial homodromy in growing shoots of perennial plants with spiral phyllotaxis is the case when chirality phyllotactic pattern does not change consecutive growth increments same axis. In conifers such as <em>Picea</em> or<em> Abies</em> this rule strictly observed, except for rare cases discontinuous transitions. <em>Torreya</em>, however, changes, at random, every year. The primordia packing, executed by vegetative shoot apical meristem (SAM), depends...
Auxin, which is transported in the outermost cell layer, one of major players involved plant organ initiation and positioning at shoot apical meristem (SAM). However, recent studies have recognized role putative internal signals as an important factor collaborating with well-described superficial pathway organogenesis regulation. Different been proposed; however, their nature transport route not precisely determined. Therefore, this mini-review, we aimed to summarize current knowledge...
In the shoot apical meristem (SAM) of Arabidopsis, PIN1-dependent polar auxin transport (PAT) regulates two crucial developmental processes: organogenesis and vascular system formation. However, knockout mutation in PIN1 gene does not fully inhibit these processes. Therefore, we investigated a potential source for vascularization during inflorescence stem development. We analyzed distribution wild-type (WT) pin1 mutant plants using refined protocol immunolocalization; activity, with response...
<p>Colonization of terrestrial ecosystems by the first land plants, and their subsequent expansion diversification, were crucial for life on Earth. However, our understanding these processes is still relatively poor. Recent intensification studies various plant organisms have identified cell walls are those structures, which played a key role in adaptive during evolution plants. Cell wall as structure protecting protoplasts showing high structural plasticity was one primary subjects to...
We report on some new aspects of the internal architecture conifer shoot, proving that it is even more strongly related to phyllotaxis than was initially thought. The number vascular sympodia in an open system <em>Abies</em> and <em>Picea</em> shoots correlated with cortical resin canals. Both numbers are consecutive members phyllotactic series, which defines pattern shoot. This means concentric systems alternately oriented canals interlocked, following course...
Techniques for in situ localization of gene products provide indispensable information understanding biological function. In the case enzymes, function is directly related to activity, and therefore, knowledge activity patterns central molecular controls plant development. We have previously developed a novel type fluorogenic substrate revealing glycoside hydrolase planta, based on resorufin β-glycosides Here, we explore wider range such substrates visualize activities Arabidopsis...
ABSTRACT In dicotyledons, xyloglucan is the major hemicellulose of primary walls affecting load-bearing framework with participation XTH enzymes. We used loss- and gain-of function approaches to study functions abundant cambial region expressed XTH4 XTH9 in secondary growth. secondarily thickened hypocotyls, these enzymes had positive effects on vessel element expansion fiber intrusive addition, they stimulated wall thickening, but reduced xylem production. Cell analyses inflorescence stems...
In conifers with spiral phyllotaxis, two numbers: one of the vascular sympodia and second cortical resin canals, define shoot anatomic diameter. This in turn reflects size vigor apical meristem. Both numbers belong to mathematical series, associated phyllotactic pattern. The number canals is step lower a series than sympodia. first one, easier determine, automatically defines second. Using this protocol screening large branching shoots selected conifers, we have discovered strong correlation...