A5088952770- Heat shock proteins research
- Vascular Malformations Diagnosis and Treatment
- Peptidase Inhibition and Analysis
- Cancer Research and Treatments
- Microtubule and mitosis dynamics
- Signaling Pathways in Disease
- Vascular Anomalies and Treatments
- Platelet Disorders and Treatments
- RNA modifications and cancer
- Alzheimer's disease research and treatments
- Vascular Malformations and Hemangiomas
- Connexins and lens biology
- 3D Printing in Biomedical Research
- Caveolin-1 and cellular processes
- Tryptophan and brain disorders
- Hippo pathway signaling and YAP/TAZ
- S100 Proteins and Annexins
- Genetics, Aging, and Longevity in Model Organisms
- Cancer-related Molecular Pathways
- Cellular transport and secretion
- Angiogenesis and VEGF in Cancer
- Endoplasmic Reticulum Stress and Disease
- Computational Drug Discovery Methods
- Glaucoma and retinal disorders
- Stress Responses and Cortisol
University of South Florida
2017-2023
USF Health Byrd Alzheimer's Institute
2017-2020
The microtubule-associated protein tau (MAPT, tau) forms neurotoxic aggregates that promote cognitive deficits in tauopathies, the most common of which is Alzheimer's disease (AD). 90-kDa heat shock (Hsp90) chaperone system affects accumulation these toxic species, can be modulated with Hsp90 inhibitors. However, many inhibitors are not blood-brain barrier-permeable, and several present associated toxicities. Here, we find cochaperone, activator ATPase homolog 1 (Aha1), dramatically...
The accumulation of amyloidogenic proteins is a pathological hallmark neurodegenerative disorders. aberrant the microtubule associating protein tau (MAPT, tau) into toxic oligomers and amyloid deposits primary pathology in tauopathies, most common which Alzheimer’s disease (AD). Intrinsically disordered proteins, like tau, are enriched with proline residues that regulate both secondary structure aggregation propensity. orientation regulated by cis/trans peptidyl-prolyl isomerases (PPIases)....
Early life stress (ELS) adversely affects the brain and is commonly associated with etiology of mental health disorders, like depression. In addition to mood-related symptoms, patients depression show dysregulation hypothalamic-pituitary-adrenal (HPA) axis, increased peripheral inflammation, structural alterations. Although underlying causes are unknown, polymorphisms in FK506-binding protein 5 (FKBP5) gene, a regulator glucocorticoid receptor (GR) activity, interact childhood adversities...
Gain-of-function mutations within the olfactomedin (OLF) domain of myocilin result in its toxic intracellular accumulation and hasten onset open-angle glaucoma. The absence does not cause disease; therefore, strategies aimed at eliminating could lead to a successful glaucoma treatment. endoplasmic reticulum Hsp90 paralog Grp94 accelerates OLF aggregation. Knockdown or pharmacological inhibition cells facilitates clearance mutant via non-proteasomal pathway. Here, we expanded our support for...
Objective: We sought to determine how endothelial cell (EC) expression of the activating k-Ras (kirsten rat sarcoma 2 viral oncogene homolog) mutation, k-RasV12, affects their ability form lumens and tubes interact with pericytes during capillary assembly Approach Results: Using defined bioassays where human ECs undergo observable tubulogenesis, sprouting behavior, pericyte recruitment EC-lined tubes, pericyte-induced EC basement membrane deposition, we assessed impact k-RasV12 on these...
Here, we examine known GTPase regulators of vesicle trafficking events to assess whether they affect endothelial cell (EC) lumen and tube formation. We identify novel roles for the small GTPases Rab3A, Rab3B, Rab8A, Rab11A, Rab27A, RalA, RalB caveolin-1 in co-regulating membrane that control EC siRNA suppression individual such as markedly inhibit tubulogenesis, while greater blockade is observed with combinations siRNAs Rab3A Rab8A RalA RalB. These also disrupt very early formation...
CTD, C-terminal domain; TD, tetramerization RD, regulatory NTD, N-terminal DBD, DNA-binding AD1, activation domain 1; VEGF, vascular endothelial growth factor History of p53 and some important events in the first 30 years since its discoveryThe gene was discovered 1979.A protein identified simian virus 40-transformed mouse cells (SV40) by immunoprecipitation with anti-T serum; this called p53. 1 In same year, Kress other scientists found a new class proteins molecular mass ranging from...