A5019607049- Platelet Disorders and Treatments
- Angiogenesis and VEGF in Cancer
- Kruppel-like factors research
- Cell Adhesion Molecules Research
- Blood Coagulation and Thrombosis Mechanisms
- Chemokine receptors and signaling
- Chronic Myeloid Leukemia Treatments
- Barrier Structure and Function Studies
- Vascular Malformations Diagnosis and Treatment
- Vascular Malformations and Hemangiomas
- Cancer Treatment and Pharmacology
- Cancer Cells and Metastasis
- Microtubule and mitosis dynamics
- Hippo pathway signaling and YAP/TAZ
University of South Florida
2019-2024
Objective: We sought to identify and investigate the functional role of major endothelial cell (EC)-derived factors that control pericyte recruitment EC tubes pericyte-induced tube maturation during capillary network formation. Approach Results: PDGF (platelet-derived growth factor)-BB, PDGF-DD, ET (endothelin)-1, TGF (transforming factor)-β, HB-EGF (heparin-binding epidermal factor), as key individual combined regulators assembly around tubes. Using novel only assays, we demonstrate...
In this work, we examine the molecular basis for capillary tube regression and identify key proregressive factors, signaling pathways, pharmacological antagonists of process. Approach Results: We demonstrate that proinflammatory mediators, IL (interleukin)-1β, TNF (tumor necrosis factor) α, thrombin, singly in combination, are potent regulators vitro. These when added to endothelial cell-pericyte cocultures, led selective loss cell-lined networks, with retention proliferation pericytes...
Objective: In this work, we have sought to define growth factor requirements and the signaling basis for different stages of human vascular morphogenesis maturation. Approach Results: Using a serum-free model endothelial cell (EC) tube in 3-dimensional collagen matrices that depends on 5 combination, SCF (stem factor), IL (interleukin)-3, SDF (stromal-derived factor)-1α, FGF (fibroblast factor)-2, insulin (factors), demonstrate VEGF (vascular factor) pretreatment ECs 8 hours (ie, priming)...
Blood vessels in different vascular beds vary size, which is essential for their function and fluid flow along the network. Molecular mechanisms involved formation of a lumen appropriate or tubulogenesis, are still only partially understood. Src homology 2 domain containing E (She) protein was previously identified screen proteins that interact with Abelson (Abl)-kinase. However, its biological role has remained unknown. Here we demonstrate She Abl signaling regulate vessel size zebrafish...
Objective: We sought to determine how endothelial cell (EC) expression of the activating k-Ras (kirsten rat sarcoma 2 viral oncogene homolog) mutation, k-RasV12, affects their ability form lumens and tubes interact with pericytes during capillary assembly Approach Results: Using defined bioassays where human ECs undergo observable tubulogenesis, sprouting behavior, pericyte recruitment EC-lined tubes, pericyte-induced EC basement membrane deposition, we assessed impact k-RasV12 on these...
Abstract Blood vessels in different vascular beds vary lumen diameter, which is essential for their function and fluid flow along the network. Molecular mechanisms involved formation of a appropriate size, or tubulogenesis, are still only partially understood. Src homology 2 domain containing E (She) protein was previously identified screen proteins that interact with Abelson (Abl)-kinase. However, its biological role has remained unknown. Here we demonstrate She Abl signaling regulate size...