A5081422350- Fungal and yeast genetics research
- Plant-Microbe Interactions and Immunity
- Plant Disease Resistance and Genetics
- Plant Virus Research Studies
- Blood groups and transfusion
- Plant pathogens and resistance mechanisms
- Genetic Mapping and Diversity in Plants and Animals
- Plant Reproductive Biology
- RNA modifications and cancer
- Plant Pathogens and Fungal Diseases
- Mycotoxins in Agriculture and Food
- Insect Resistance and Genetics
- Mycorrhizal Fungi and Plant Interactions
- Insect symbiosis and bacterial influences
- Polysaccharides and Plant Cell Walls
- Wheat and Barley Genetics and Pathology
- Entomopathogenic Microorganisms in Pest Control
- Plant Pathogens and Resistance
- Erythrocyte Function and Pathophysiology
Max Planck Institute for Terrestrial Microbiology
2006-2023
Max Planck Society
2014
Philipps University of Marburg
1997
Ustilago maydis is an important fungal pathogen of maize, causing corn smut. It well adapted to its host and proliferates in living plant tissue without inducing a defence response. The genome sequence U. has now been determined, the first for biotrophic parasite. Several gene clusters that encode secreted proteins unknown function were identified: genome-wide expression analysis shows clustered genes are upregulated during disease. Mutations these frequently affect virulence, ranging from...
Biotrophic pathogens, such as the related maize pathogenic fungi Ustilago maydis and Sporisorium reilianum, establish an intimate relationship with their hosts by secreting protein effectors. Because secreted effectors interacting plant proteins should rapidly evolve, we identified variable genomic regions sequencing genome of S. reilianum comparing it U. genome. We detected 43 low sequence conservation in otherwise well-conserved syntenic genomes. These primarily encode include previously...
Infection-related development of phytopathogenic fungi is initiated by sensing and responding to plant surface cues. This response can result in the formation specialized infection structures, so-called appressoria. To unravel program inducing filaments appressoria biotrophic smut fungus Ustilago maydis, we exposed cells a hydrophobic cutin monomer 16-hydroxy hexadecanoic acid. Genome-wide transcriptional profiling at pre-penetration stage documented dramatic changes almost 20% genes....
The biotrophic basidiomycete fungus Ustilago maydis causes smut disease in maize. Hallmarks of the are characteristic large tumors which dark pigmented spores formed. Here, we functionally characterized a novel core effector lep1 (late protein 1) is highly expressed during tumor formation and contributes to virulence. We characterize mutants, localize protein, determine phenotypic consequences upon deletion as well constitutive expression, analyze relationships with repellent Rep1...
Summary The biotrophic fungus Ustilago maydis causes the smut disease of maize. interaction with its host and induction characteristic tumors are governed largely by secreted effectors whose function is mostly unknown. To identify a prominent role in virulence, we used RNA sequencing found that gene sta1 upregulated during early stages infection. We characterized Sta1 comparative genomics, reverse genetics, protein localization, stress assays, microscopy. mutants show dramatic reduction...
The easyPACId (easy Promoter Activation and Compound Identification) approach is focused on the targeted activation of natural product biosynthetic gene clusters (BGCs) encoding non-ribosomal peptide synthetases (NRPS), polyketide synthases (PKS), NRPS-PKS hybrids, or other BGC classes. It was applied to entomopathogenic bacteria genera Xenorhabdus Photorhabdus by exchanging promoter desired BGCs against L-arabinose inducible PBAD in ∆hfq mutants respective strains. crude (culture) extracts...
The tight interaction between pathogens and their hosts results in reciprocal selective forces that impact the genetic diversity of interacting species. footprints this selection differ pathosystems because distinct life-history traits, demographic histories, or genome architectures. Here, we studied genome-wide patterns 22 isolates causative agent corn smut disease, Ustilago maydis, originating from five locations Mexico, presumed center origin In species, many genes encoding secreted...
Abstract The tight interaction between pathogens and their hosts results in reciprocal selective forces that impact the genetic diversity of interacting species. footprints this selection differ pathosystems because distinct life-history traits, demographic histories, or genome architectures. Here, we studied genome-wide patterns 22 isolates causative agent corn smut disease, Ustilago maydis , originating from five locations Mexico, presumed center origin In species, many genes encoding...