A5046081346- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Fungal and yeast genetics research
- Plant Parasitism and Resistance
- Mycorrhizal Fungi and Plant Interactions
- Plant Reproductive Biology
- Insect-Plant Interactions and Control
- Microtubule and mitosis dynamics
- Essential Oils and Antimicrobial Activity
- Radio Astronomy Observations and Technology
- Legume Nitrogen Fixing Symbiosis
- Congenital heart defects research
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Plant tissue culture and regeneration
- Transgenic Plants and Applications
- Plant pathogens and resistance mechanisms
- Genetic Mapping and Diversity in Plants and Animals
Okayama University
2021-2024
Kyoto Prefectural University
2015-2022
Max Planck Institute for Terrestrial Microbiology
2018-2021
Morphogenesis in filamentous fungi depends on appropriate cell cycle progression. Here, we report that cells of the cucumber anthracnose fungus Colletotrichum orbiculare regulate G1/S progression via a two-component GAP, consisting Budding-uninhibited-by-benomyl-2 (Bub2) and Byr-four-alike-1 (Bfa1) as well its GTPase Termination-of-M-phase-1 (Tem1) to establish successful infection. In random insertional mutagenesis screen infection-related morphogenesis, isolated homolog Saccharomyces...
The biotrophic basidiomycete fungus Ustilago maydis causes smut disease in maize. Hallmarks of the are characteristic large tumors which dark pigmented spores formed. Here, we functionally characterized a novel core effector lep1 (late protein 1) is highly expressed during tumor formation and contributes to virulence. We characterize mutants, localize protein, determine phenotypic consequences upon deletion as well constitutive expression, analyze relationships with repellent Rep1...
To breach the plant cuticle, many pathogenic fungi differentiate specialized infection structures (appressoria). In Colletotrichum orbiculare (cucumber anthracnose fungus), this differentiation requires unique proper G1 /S phase progression, regulated by two-component GTPase activating protein CoBub2/CoBfa1 and CoTem1. Since their homologues regulate mitotic exit, cytokinesis, or septum formation from yeasts to mammals, we asked whether BUB2 function in progression is specific fungi....
Plants grow in association with diverse microorganisms. During communication between plants and microbes, beneficial phytopathogenic bacteria fungi emit various volatile compounds (VCs). These microbial VCs (mVCs) are typically small, odorous low boiling point, high vapor pressure, a lipophilic moiety (Schulz Dickschat, 2007). Based on recent studies, mVCs appear to regulate plant nutrient acquisition, photosynthesis, phytohormone actions, metabolic processes, leading an improvement...
Plants employ two different types of immune receptors, cell surface pattern recognition receptors (PRRs) and intracellular nucleotide-binding leucine-rich repeat-containing proteins (NLRs), to cope with pathogen invasion. Both often share similar downstream components responses but it remains unknown whether a PRR an NLR assemble into the same protein complex or distinct receptor complexes. We have previously found that small GTPase OsRac1 plays key roles in signaling OsCERK1, for fungal...
Many biotrophic and hemibiotrophic fungal pathogens use appressoria to directly penetrate the host plant surface. In cucumber anthracnose fungus Colletotrichum orbiculare, differentiation of requires a proper G1/S cell cycle progression, regulated by GTPase-activating protein complex CoBub2-CoBfa1 its downstream GTPase CoTem1. To explore mechanisms which CoTem1 cascade regulates infection, we screened for interaction factors identified Niemann-Pick type C2 homolog (CoNpc2). C proteins NPC1...
Abstract Organophosphate is the commonly used pesticide to control pest outbreak, such as those by aphids in many crops. Despite its wide use, however, necrotic lesion and/or cell death following application of organophosphate pesticides has been reported occur several species. To understand this phenomenon, called sensitivity (OPS) sorghum, we conducted QTL analysis a recombinant inbred line derived from Japanese cultivar NOG, which exhibits OPS. Mapping OPS population identified prominent...
Abstract NLR family proteins act as intracellular receptors. Gene duplication amplifies the number of genes, and subsequent mutations occasionally provide modifications to second gene that benefits immunity. However, evolutionary processes after functional relationships between duplicated NLRs remain largely unclear. Here, we report rice protein Pit1 is associated with its paralogue Pit2. The two are required for resistance blast fungus but have different functions: induces cell death, while...
The unique role of cell cycle regulators in Colletotrichum orbiculareColletotrichum orbiculare is the causal agent cucumber anthracnose disease.The infection process initiated through recognition specific surface cues by aseptate conidia.Following germination conidia, emerging germ tubes differentiate into dome-shaped appressoria, which subsequently undergo nuclear division (Kubo and Takano 2013; et al. 2001).From random geneinsertional mutagenesis C. orbiculare, CoBUB2 gene was identified...