A5073135785- Bone Tissue Engineering Materials
- Periodontal Regeneration and Treatments
- Dental Implant Techniques and Outcomes
- Dental materials and restorations
- RNA Interference and Gene Delivery
- Endodontics and Root Canal Treatments
- Electrospun Nanofibers in Biomedical Applications
- Oral microbiology and periodontitis research
- Dental Research and COVID-19
- Photodynamic Therapy Research Studies
- Bone Metabolism and Diseases
- Advanced biosensing and bioanalysis techniques
- Orthopaedic implants and arthroplasty
- Bone health and osteoporosis research
- Advanced Nanomaterials in Catalysis
- Silk-based biomaterials and applications
- Craniofacial Disorders and Treatments
- Collagen: Extraction and Characterization
- Knee injuries and reconstruction techniques
- Bone fractures and treatments
- Graphene and Nanomaterials Applications
- RNA Research and Splicing
- Dental Radiography and Imaging
- Mesenchymal stem cell research
- Titanium Alloys Microstructure and Properties
Tohoku University
2015-2025
Redox (Norway)
2019
Hokkaido University
2005-2012
This study aimed to fabricate a growth factor-releasing biodegradable scaffold for tissue regeneration. We prepared multishell calcium phosphate (CaP) nanoparticles functionalized with DNA, polyethyleneimine (PEI), protamine and octa-arginine (R8) compared their respective transfection activity cell viability measures using human mesenchymal stem cells. DNA-protamine complexes improved the efficiency of CaP exception those R8. These also greatly reduced cytotoxicity PEI. In addition, we...
Abstract The purpose of the present study was to confirm if proanthocyanidin-rich grape seed extract (GSE) had ability improve bone health such as loss, healing, and implant osseointegration (defined direct connection between tissue an implant) in ovariectomized (OVX) animals. We demonstrated that daily oral administration GSE prevented loss lumbar vertebrae femur OVX mice. In addition, osteoclastogenesis spine mice, assessed by histological histomorphometric analyses, accelerated but this...
Objectives: This study aimed to evaluate the effectiveness of fully remote practical training for preparation removable partial dentures, using video meeting platform Zoom and communication app. LINE. Materials Methods: Remote was applied one student LINE at same time as in-person rest class. Consumable instruments needed were delivered subject in advance. The student’s work assessed front a web camera, pre-determined checkpoint during each step. actual appliances then mailed instructors...
Abstract The aim of this study was to investigate the bactericidal effect and recovery biocompatibility contaminated titanium surfaces using a combination treatment involving silver, copper, or iron ion application along with 400 nm purple-LED light irradiation. Additionally, sought develop functional calcium phosphate (CaP) coating on following disinfection, promote re-osseointegration. A emitting at utilized irradiate Staphylococcus aureus suspensions biofilms in presence various...
This study evaluated apical periodontal healing after root-end sealing using 4-META/MMA-TBB resin (SB), and filling reinforced zinc oxide eugenol cement (EBA) or mineral trioxide aggregate (MTA) when root canal infection persisted. Apical periodontitis was induced in mandibular premolars of beagles by contaminating the canals with dental plaque. After 1 month, SB group, applied to resected surface following apicoectomy. In EBA MTA groups, a cavity prepared filled MTA. control not filled....
Zirconia-based dental restorations are becoming used more commonly. However, limited attention has been given to the difficulties experienced, concerning cutting, in removing when needed. The aim of present study was compare cutting efficiency diamond burs, operated using an electric high-speed handpiece, on zirconia (Zir) with those lithium disilicate glass-ceramic (LD) and leucite (L). In addition, evaluation burs Zir different thicknesses performed. Specimens were prepared 0.5, 1.0, 2.0,...
Abstract Titanium dental implants have been successfully used for decades; however, some are affected by peri-implantitis due to bacterial infection, resulting in loss of supporting bone. This study aimed evaluate the effect an antimicrobial chemotherapy employing H 2 O photolysis—developed treat peri-implantitis—on biofilm-contaminated titanium surfaces association with osteoblastic cell proliferation on treated surface. discs were sandblasted and acid-etched, followed contamination a...
Cementum shares many properties with bone; however, in contrast to bone, it is not innervated or vascularized and has a limited capacity for remodeling. Osteocytes located the lacunae-canalicular system of bone tissue play central role remodeling by communicating osteoblasts osteoclasts. Although cementocytes are present cellular cementum morphologically similar osteocytes, remains unclear whether they involved dynamic functional regulation metabolism cementum. The study focused on...
Nanoparticles represent promising gene delivery systems in biomedicine to facilitate prolonged expression with low toxicity compared viral vectors. Specifically, nanoparticles of calcium phosphate (nCaP), the main inorganic component human bone, exhibit high biocompatibility and good biodegradability have been reported affinity for protein or DNA, having thus used as transfer On other hand, Octa-arginine (R8), which has a permeability cell membrane, improve intracellular systems. Here, we...
During tissue repair or regeneration, several bioactive molecules are released and interact with each other act as complex additives inhibitors for reconstruction. In this study, the bone-healing effects of combination treatment tumor necrosis factor-α (TNF-α) inhibition, vascular endothelial growth factor A (VEGF-A) bone morphogenetic protein-7 (BMP-7) release by gene silencing, transfection calcium phosphate nanoparticles (CaP) in rat femoral head was histologically, morphologically,...
Abstract This study investigated the bactericidal effect, underlying mechanisms of treatment, and recovery biocompatibility infected titanium surface using a combination treatment silver ion application ultraviolet-A (UV-A) light irradiation. Streptococcus mutans Aggregatibacter actinomycetemcomitans were used in suspension as biofilm on to test for effect. The effect was significantly higher than that or UV-A irradiation alone. attributable hydroxyl radicals, which generated from bacterial...
The objective of the present study was to evaluate effectiveness introducing integrated jaw models, rubric criteria and homework tasks a total clinical simulation training course improve competence preclinical dental students.A course, which involved six dentistry departments, held for 110 students in 2014 2015. We prepared models having several morbidities along with corresponding medical information tasks. formulated diagnoses devised treatment plans before performing on mannequin under...
Periodontal ligament is regenerated in association with hard tissue regeneration. Tenomodulin (Tnmd) expression has been confirmed periodontal and it reportedly inhibits angiogenesis or involved collagen fibril maturation. The introduction of Tnmd by gene transfection bone regeneration therapy might inhibit topical formation induce the dense fibrous tissue. Therefore, effect technique vitro vivo was investigated this study. Osteogenesis- chondrogenesis-related levels osteoblastic cells...
Many β-Ti alloys have been developed for, and used in, medical devices because of the corrosion resistance, biocompatibility, exceptionally low Young’s modulus. The aim present study was to investigate histomorphometric aspects peri-implant bone around Ti-Nb-Sn alloy implants compare them with those in case commercially pure Ti (Ti). Fluorescent morphological observations ST-2 cells on substrate were performed morphogenesis rat femur evaluated. There no difference between cell morphology...
An antimicrobial technique utilizing hydroxyl radicals generated by the photolysis of 3% H2O2 has been developed recently. The present study aimed to evaluate effect treatment on tooth demineralization caused Streptococcus mutans biofilm.To induce demineralization, S. biofilm was allowed form maxillary first molars collected from Wistar rats via 24-h culturing. samples were immersed in and irradiated with 365-nm LED (H2O2 treatment). Viable bacterial counts evaluated immediately after an...
本研究の目的は, 象牙質面にBMP-2処理する前の脱灰方法の違いが, 培養細胞のALP活性, および結合組織内での象牙質表面への硬組織形成と象牙質吸収に及ぼす影響を検討することである。実験1では, 象牙質片をEDTA (pH7.0), 塩酸テトラサイクリン (pH2.0), クエン酸 (pH1.0) で3分間脱灰後, 0, 5, 10μg/mlのrhBMP-2に10分間浸漬して, 歯根膜細胞を播種, 培養し, 1, 3, 5日後にALP活性を測定した。実験2では, 象牙質片をEDTA, 塩酸テトラサイクリン, クエン酸, PBSに3分間浸漬後, 100, 400μg/mlのrhBMP-2に10分間浸漬し, ラット口蓋結合組織に移植して2, 4週後に象牙質片上に形成された硬組織の形成率と象牙質の吸収率を計測した。実験1の結果, BMP-2が10μg/mlの場合にEDTA群のALP活性が他の2群と比較して有意に高かった。実験2の結果, 硬組織形成率はBMP-2濃度が100μg/mlの場合にEDTA群が他の3群と比較して有意に高く,...