A5038428640- Neurobiology and Insect Physiology Research
- Genetics, Aging, and Longevity in Model Organisms
- Neuroscience and Neuropharmacology Research
- Mitochondrial Function and Pathology
- Signaling Pathways in Disease
- Cell death mechanisms and regulation
- Invertebrate Immune Response Mechanisms
- Metabolism and Genetic Disorders
- Insect Resistance and Genetics
- Cholinesterase and Neurodegenerative Diseases
- Alzheimer's disease research and treatments
- Animal Behavior and Reproduction
- Asthma and respiratory diseases
- Lipid metabolism and biosynthesis
- Hippo pathway signaling and YAP/TAZ
- Developmental Biology and Gene Regulation
- RNA Research and Splicing
- Insect and Arachnid Ecology and Behavior
- Cancer, Hypoxia, and Metabolism
- Redox biology and oxidative stress
- Viral Infectious Diseases and Gene Expression in Insects
- Hepatocellular Carcinoma Treatment and Prognosis
- Tracheal and airway disorders
- Liver physiology and pathology
- Endoplasmic Reticulum Stress and Disease
Kanagawa University of Human Services
2011-2025
Mitsubishi Tanabe Pharma Corporation
2025
Suita Municipal Hospital
2019-2021
Tokyo Metropolitan University
1999-2015
Osaka City General Hospital
2012
Japan Biological Informatics Consortium
2006-2007
Wakayama Prefectural Tanabe High School
1997-1999
Osaka University
1992-1998
Tanabe Research Laboratories
1997
Institute of Applied Biochemistry
1994
To study the molecular mechanisms underlying neuronal programmed cell death (PCD), we performed differential display screening for genes, expression of which was induced during PCD in sympathetic neuron culture model deprived NGF. We cloned a gene encoding novel polypeptide (DP5) consisted 92 amino acids. DP5 had no homology with any other known protein and contained motif that would indicate its putative biochemical functions. mRNA levels peaked at 15 h after nerve growth factor withdrawal,...
To elucidate the molecular mechanisms underlying neuronal death after transient forebrain ischemia, we cloned genes expressed ischemia in Mongolian gerbil by a differential display method. A homolog of rat zinc transporter, ZnT-1, which transports intracellular Zn 2+ out cells, was isolated. Its expression became detectable exclusively pyramidal neurons CA1 region 12 hr and reached maximum from day 1 to 2 as shown situ hybridization. By 7, had disappeared entirely cells region, because died....
DP5, which contains a BH3 domain, was cloned as neuronal apoptosis-inducing gene. To confirm that DP5 interacts with members of the Bcl-2 family, 293T cells were transiently co-transfected and Bcl-xl cDNA constructs, immunoprecipitation carried out. The 30-kDa co-immunoprecipitated Myc-tagged suggesting physically in mammalian cells. Previously, we reported is induced during apoptosis cultured sympathetic neurons. Here, analyzed gene expression specific interaction death by amyloid-β protein...
Sleep is a fundamental biological process for all animals. However, the molecular mechanisms that regulate sleep are still poorly understood. Here we report sleep-like behavior in Drosophila severely impaired by mutations sarah ( sra ), member of Regulator Calcineurin (RCAN) family genes. reduction mutants highly correlated with decreases Sra protein levels. Pan-neural expression rescues this behavioral phenotype, indicating neuronal function required normal sleep. Since regulates...
The Drosophila melanogaster sex-peptide (melSP) is a seminal fluid component that induces postmating responses (PMR) of females via the receptor (SPR) . Although SP orthologs are found in many species, their functions remain poorly characterized. It unknown whether conserved across species or rather specific to each species. Here, we developed GFP-tagged melSP (G-SP) and used it visualize cross-species binding activity female reproductive system various First demonstrated ectopically...
Identification of sensitized allergens for patients with respiratory allergy is an important step in disease care and environmental allergen control. The Japanese archipelago belongs to various climate categories due its length from north south which transverse the subarctic subtropical south, suggesting substantial regional differences dominant allergens. However, few studies have assessed prevalence sensitization allergens.We requested three major clinical testing laboratories provide us...
Visual inspectors′ ability to detect foreign matter in injections must be qualified for each product type or bracketing group, as stated the United States Pharmacopeia <1790>. The common defect criterion is ″visible″ However, this qualitative lower rejection limit based on premise that nature of human inspection at one site globally comparable all other sites. If not, can vary among inspector groups product, leading quality differences between Inspectors are trained and using samples...
Parkin-associated endothelin receptor-like receptor (Pael-R) is a substrate of the E3 ubiquitin ligase Parkin, which has been implicated in pathogenesis Parkinson disease. Misexpression human Pael-R Drosophila shown to induce selective loss dopaminergic neurons, symptom Using this model, we investigated whether thioredoxin (TRX), an evolutionarily conserved antioxidant and molecular chaperone, could suppress neurotoxicity induced by Pael-R. The genome contains three TRX-encoding genes,...
Overexpression of thioredoxin (TRX) confers oxidative stress resistance and extends lifespan in mammals insects. However, less is known about phenotypes associated with loss TRX. We investigated loss‐of‐function Trx‐2 Drosophila , found that the mutant flies are hyper‐susceptible to paraquat, a free radical generator, but not hydrogen peroxide. They contain high amount protein carbonyl, which dramatically increases age. mutants express levels anti‐oxidant genes, such as superoxide dismutase...
Plenty of SH3s (POSH) functions as a scaffold protein for the Jun N-terminal kinase (JNK) signal transduction pathway, which leads to cell death in mammalian cultured cells and Drosophila. Here, we show that POSH forms complex with Apoptosis-linked gene-2 (ALG-2) ALG-2-interacting (ALIX/AIP1) calcium-dependent manner. Overexpression ALG-2 or ALIX developing imaginal eye discs resulted roughened melanized eyes, respectively. These phenotypes were enhanced by co-overexpression POSH. We found...
The Drosophila sex-peptide (SP) has been identified as a seminal fluid component that induces post-mating responses (PMRs) in the inseminated females, such inhibition of remating and stimulation egg-laying. SP thought to play central role sexual conflict sexually antagonistic co-evolution. Most sequenced genomes contain orthologs, but their functions have poorly characterized. Recently, we investigated cross-species activity D. melanogaster by means injection into virgin females other...
Insulin‐degrading enzyme (IDE) is implicated in the pathogenesis of type 2 diabetes mellitus (DM2) and Alzheimer's disease (AD). Here we provide genetic evidence that Drosophila Ide ( dIde ) antagonizes insulin signaling pathway human Aβ‐induced neurotoxicity . In this study, also generated a knockout mutant KO by gene targeting, found loss IDE increases content major insect blood sugar, trehalose, thus suggesting conserved role sugar metabolism. Using as model, further investigations into...
Abstract To better understand the genetic bases of postmating responses in Drosophila melanogaster females, we screened a collection P{GS} insertion lines and identified two insertions sarah (sra), whose misexpression nervous system induced high levels ovulation virgins. The gene sra encodes protein similar to human Down syndrome critical region 1 (DSCR1). phenotype was reproduced transgenic virgins expressing UAS-sra system. flies also extruded ovipositor toward courting males as seen...
Insulin/insulin-like growth factor (IGF) plays an important role as a systemic regulator of metabolism in multicellular organisms. Hyperinsulinemia, high level blood insulin, is often associated with impaired physiological conditions such hypoglycemia, insulin resistance, and diabetes. However, due to the complex pathophysiology hyperinsulinemia, causative excess insulin/IGF signaling has remained elusive. To investigate biological effects metabolic homeostasis physiology, we generated flies...