A5049548088- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Sperm and Testicular Function
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- Genital Health and Disease
- Reproductive System and Pregnancy
- Transgenic Plants and Applications
- Plant and Fungal Species Descriptions
- Food Allergy and Anaphylaxis Research
- Herpesvirus Infections and Treatments
- Genomics and Phylogenetic Studies
- Toxin Mechanisms and Immunotoxins
- Urologic and reproductive health conditions
- Microbial Metabolism and Applications
- T-cell and B-cell Immunology
- Reproductive Biology and Fertility
- Plant tissue culture and regeneration
- Hepatitis C virus research
- Carbohydrate Chemistry and Synthesis
- Cell Adhesion Molecules Research
- Immunotherapy and Immune Responses
- Animal Genetics and Reproduction
- Complement system in diseases
Kunming Institute of Zoology
1993-2011
Chinese Academy of Sciences
1986-2011
Kunming University
2011
National Institute of Infectious Diseases
2002
117th Hospital of People's Liberation Army
2002
Shanghai Public Security Bureau
2002
Luxun Academy of Fine Arts
2002
Women’s Environmental Institute
1999
Yunnan Yingmao Biotechnology (China)
1992
Oregon National Primate Research Center
1986
Objectives To investigate the molecular epidemiology of HIV in China's Yunnan Province, where initial HIV-1 outbreak among injecting drug users (IDU) occurred 1989, and to analyse genesis interrelationship epidemic with that surrounding areas. Design A epidemiological investigation was conducted IDU three prefectures including Wenshan (east), Honghe (southeast) Dehong (west). Methods Thirty-nine specimens were collected from consenting 2000–2001. The nucleotide sequences 2.6 kb gag-RT 340...
The southeastern region of Yunnan province is a key site for drug trafficking and HIV-1 infection spread from the west Laos to China. To investigate prevalence hepatitis C virus (HCV) coinfection among injection users (IDUs) in Yunnan, three cohorts 285 addicts, including 242 IDUs 43 oral users, living cities Gejiu Kaiyuan county Yanshan were studied. HCV infections detected by enzyme-linked immunosorbent assay and/or polymerase chain reaction. Data on age, sex, risk behavior, use history,...
Trichosanthin (TCS) is a ribosome-inactivating protein from root tubers of Trichosanthes kirilowii Maxim. In this paper, the effects TCS on viability human peripheral blood immunocytes, proliferation lymphocytes, and its cytotoxicity to twelve cell lines lymphoma or leukemia had been observed. at high concentration (>12.5 μg/ml) affected B but not that mononuclear cells (PBMCs), T lymphocytes granulocytes. Human blood-derived monocytes/macrophages were hrghly sensitive (ID50 1.70 μg/ml)....
ABSTRACT We identified a new class of human immunodeficiency virus type 1 (HIV-1) recombinants (00CN-HH069 and 00CN-HH086) in which further recombination occurred between two established circulating recombinant forms (CRFs). These isolates were found among 57 HIV-1 samples from cohort injecting drug users eastern Yunnan Province China. Informative-site analysis conjunction with bootscanning plots exploratory tree revealed that these strains closely related mosaics comprised CRF07_BC...
The generation of a new, cost-effective, non-primate, small-animal model would greatly facilitate research into hepatitis C virus (HCV) pathogenesis and the development novel therapeutic preventative technologies to control increasing HCV threat public health. Native from patient plasma grown in cell culture (HCVcc) were used inoculate adult tree shrews. Each animal was inoculated with one genotype. Alanine aminotransferase (ALT) levels, RNA viral load determined animals before after...
Molecular epidemiological investigation was conducted among injecting drug users (IDUs) (n = 11) and heterosexuals 15) in Kunming, Yunnan Province of China. HIV-1 genotypes were determined based on the nucleotide sequences 2.6-kb gag-RT region. The distribution IDUs as follows: CRF07_BC (5/11) CRF08_BC (5/11); subtype B' (1/11). Similarly, a majority Kunming (14/15) infected with (4/15), (6 /15), or known to predominate This contrasts trends coastal regions China surrounding southeastern...
A rosy-pigmented Gram-negative, thermophilic bacterium with an optimum growth temperature of about 55 degrees C was isolated from Tengchong hot springs in Yunnan province, China. Its scarcely occurred below 40 or above 70 C. Phylogenetic and secondary structural analyses 16S rRNA DNA-DNA hybridization showed that the organism represented a new species genus Meiothermus. This could be distinguished easily other Meiothermus by following phenotypic characteristics: rosy pigment, expanded body,...
A yellow-pigmented strain of the genus Thermus, with optimum growth temperatures about 65 ∼ 70 °C, was isolated from hot springs in Rehai Tengchong, Yunnan Province, China. Morphological, physiological and biochemical characteristics, pigment analysis RH99-GF7504 its phylogenetic 16S rDNA showed that this organism represented a new species Thermus. This had maximum for below 80 °C. The isolate Tengchong could be distinguished other strains Thermus by special structure inability to hydrolyze...
ABSTRACT: Male BALB/c mice at 8 to 14 weeks of age were divided into three groups: group 1 was immunized with an emulsion testicular cells (TCs) (10 7 /mouse), complete Freund's adjuvant (CFA), and extract Bordetella pertussis (BP); 2 given CFA BP injections; 3 sterile saline injections. Suspensions TCs spleen (SCs) from each mouse prepared 4 after the first immunization for a cytotoxic T lymphocyte (CTL) assay. For antibody‐dependent cell‐mediated cytotoxicity (ADCC) complement‐mediated...
The root of Cynanchum otophyllum-also known as Qing Yang Sheng-is a traditional ethnical Chinese medicine. objective this study was to evaluate in vitro activities and safety C. otophyllum glucan sulfate (PS20) against Human Immunodeficiency Virus (HIV) Herpes Simplex (HSV).Anti-HIV activity detected with syncytial formation assay quantitative P24 Enzyme-Linked Immunosorbent Assay (ELISA). Anti-HSV plaque reduction assay; cytotoxicity tested MTT colorimetric anti-bacterial microdilution...
Ab levels in the genital tract may be important fertility and preventing sexually transmitted diseases. In this study, 125I-labeled polymer or monomer mAb IgA (C4pIgA C4mIgA) IgG2b (C4IgG) to murine lactate dehydrogenase C4 a (nplgA) not cross-reacting with mouse sperm were intravenously injected into BALB/c mice, relative distribution of these Abs was determined. Polymer transported much more efficiently tract, trachea, duodenum both sexes than C4IgG mIgA (p < 0.01). The transport npIgA)...
The anti-HIV-1 neutralizing antibody assay is widely used in AIDS vaccine research and other experimental clinical studies. vital dye staining method applied the detection of has been many laboratories. However, unknown factor(s) sera or plasma affected cell growth caused protection when tested was continuously maintained culture. In addition, poor solubility neutral red medium (such as RPMI-1640) also limited use this assay. study, human T line C8166 host cells,...
The sequences of the 16S rRNA genes from 38 strains family Thermaceae were compared by alignment analysis. genus-specific and species-specific base substitutions or deletions (signature positions) found in three hypervariable regions (in helices 6, 10 17). differentiation secondary structures high variable 5′ end (38–497) containing several signature positions further supported concept. Based on comparisons segments rRNAs, a key to species was proposed.
To study anti-HIV activity and mechanism of Cynanchum otophyllum glucan sulfate in vitro.Anti-HIV-1 was detected with syncytial formation assay quantitative P24 enzyme-linked immunosorbent (ELISA); cytotoxicity tested MTT colorimetric assay. Antiviral investigated by fusion inhibition, time addition pre-treatment experiments.The 50% inhibition concentrations (IC50) PS20 for HIV-1(IIIB), HIV-1(Ada-M), HIV-1(Bal), were 0.26, 0.46, 0.90 micromol x L(-1), respectively. Studies on antiviral...