A5077402948- Mass Spectrometry Techniques and Applications
- Ion-surface interactions and analysis
- Advanced Proteomics Techniques and Applications
- Analytical Chemistry and Sensors
- Metabolomics and Mass Spectrometry Studies
- Analytical chemistry methods development
- Pesticide Residue Analysis and Safety
- Laser-induced spectroscopy and plasma
- Analytical Chemistry and Chromatography
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Capillary Electrophoresis Applications
- Nuclear Physics and Applications
- Advanced Optical Sensing Technologies
- Pharmacological Effects and Assays
- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced Fluorescence Microscopy Techniques
- Atomic and Molecular Physics
- Scientific Measurement and Uncertainty Evaluation
- Advanced Electron Microscopy Techniques and Applications
- Atomic and Subatomic Physics Research
- Spectroscopy and Laser Applications
- Bacteriophages and microbial interactions
- Advanced Measurement and Metrology Techniques
- Radioactive Decay and Measurement Techniques
- Genomics and Phylogenetic Studies
Thermo Fisher Scientific (Germany)
2015-2024
Thermo Fisher Scientific (Canada)
2018-2023
Prokhorov General Physics Institute
2010
The growing trend toward high-throughput proteomics demands rapid liquid chromatography-mass spectrometry (LC-MS) cycles that limit the available time to gather large numbers of MS/MS fragmentation spectra required for identification. Orbitrap analyzers scale performance with acquisition and necessarily sacrifice sensitivity resolving power deliver higher rates. We developed a new mass spectrometer combines mass-resolving quadrupole, Orbitrap, novel Asymmetric Track Lossless (Astral)...
Accurate mass analysis can provide useful information on the stoichiometry and composition of protein-based particles, such as virus-like assemblies. For applications in nanotechnology medicine, nanoparticles are loaded with foreign cargos, making accurate essential to define cargo load. Here, we describe modifications an Orbitrap spectrometer that enable high several up 4.5 MDa mass. This allows determination particles. The modified instrument is utilized determine load bacterial encapsulin...
Ion traps are routinely directly coupled to mass analyzers, where they serve suitably cool and shape an ion population prior pulsed extraction into the analyzer proper. Such devices benefit from high duty cycle transmission but suffer slow processing times caused by a compromise in buffer gas pressure range that dampens kinetic energy without causing excessive scatter during or within analyzer. A rectilinear RF quadrupole trap has been characterized, conjoining pressurized collision region...
Abstract The growing trend towards high-throughput proteomics demands rapid liquid chromatography-mass spectrometry (LC-MS) cycles that limit the available time to gather large numbers of MS/MS fragmentation spectra required for identification. Orbitrap analyzers scale performance with acquisition time, and necessarily sacrifice sensitivity resolving power deliver higher rates. We developed a new mass spectrometer combines quadrupole, novel Asymmetric Track Lossless (Astral) analyzer. hybrid...
Abstract Space charge effects are the Achilles' heel of all high‐resolution ion optical devices. In time‐of‐flight mass analyzers, these may manifest as reduction resolving power, measurement shift, peak coalescence, and/or transmission losses, while highly sensitive modern sources and injection devices ensure that such limits easily exceeded. have been investigated, by experiment simulation study, for astral multi‐reflection analyzer, incorporating focusing via a pair converging mirrors,...
The quality of data in charge detection mass spectrometry depends on accurate determination ion charge. While the method selective temporal overview resonant ions (STORI) has proven to be highly enabling for determining that survive variable amounts time, it assumes frequency exactly matches being used calculation. Any mismatches result low estimates. To address this, misSTORI was developed correct these discrepancies. This can significantly reduce measurement errors samples with unstable...
This Article introduces a new computationally efficient noise-tolerant signal processing method, referred to as phased spectrum deconvolution method (ΦSDM), designed for Fourier transform mass spectrometry (FT MS). ΦSDM produces interference-free spectra with resolution beyond the (FT) uncertainty limit. With presumption that oscillation phases are preserved, deconvolves an observed FT into distribution of harmonic components bound fixed frequency grid, which is several times finer than FT....
We demonstrate a method for determining the collision cross-sections (CCSs) of protein ions based on decay rate time-domain transient signal from an Orbitrap mass analyzer. Multiply charged ubiquitin, cytochrome c, and myoglobin were generated by electrospray ionization both denaturing solutions ones with high salt content to preserve native-like structures. A linear relationship between pressure in analyzer was established used that is primarily due ion-neutral collisions across entire...
A popular method for peptide quantification relies on isobaric labeling such as tandem mass tags (TMT), which enables multiplexed proteome analyses. Quantification is achieved by reporter ions generated fragmentation in a spectrometer. However, with higher degrees of multiplexing, the smaller differences between increase resolving power requirements. This contrasts faster sequencing capabilities enabled lowered resolution Orbitrap instruments. It therefore important to determine limits...
The measurement of collision cross sections (CCS, σ) offers supplemental information about sizes and conformations ions beyond mass analysis alone. We have previously shown that CCSs can be determined directly from the time-domain transient decay in an Orbitrap analyzer as oscillate around central electrode collide with neutral gas, thus removing them ion packet. Herein, we develop modified hard model, deviating prior FT-MS sphere to determine a function center-of-mass energy analyzer. With...
Measurement of collision cross section (CCS), a parameter reflecting an ion's size and shape, alongside high-resolution mass analysis extends the depth molecular by providing structural information beyond alone. Although these measurements are most commonly undertaken using dedicated ion mobility cell coupled to spectrometer, alternative methods have emerged extract CCSs directly decay rates either time-domain transient signals or FWHM frequency domain peaks in FT analyzers. This is also...
Mass spectrometry (MS)-based thermal stability assays have recently emerged as one of the most promising solutions for identification protein-ligand interactions. Here, we investigated eight combinations several introduced MS-based advancements, including Phase-Constrained Spectral Deconvolution Method, Field Asymmetric Ion Mobility Spectrometry, and implementation a carrier sample improved acquisition approaches (iMAATSA). We used intact Jurkat cells treated with commercially available MEK...
Thermo Scientific™ Orbitrap™ analyzers represent prominent class of high-resolution mass analyzer commonly used in life sciences, and for interrogation complex samples. Injected ions, trapped within a quadro-logarithmic field, orbit central electrode oscillate up down the axis. A new multi-reflection time-of-flight has been developed based on Orbitrap field structure plus an additional series periodic lenses wrapped around axis to constrain beam dispersion. The axial angular velocity...
The multireflection ion traps with isochronous properties offer a lot of opportunities for time-of-flight mass spectrometry by elongation the path, thus preserving compact dimensions an instrument. We have built and tested two-mirror linear trap that provides at least 80,000 mass-resolving power. Although resolution appears promising, there are substantial limitations arise from Coulomb interactions trapped ions. Among these, mutual repulsion ions same or close mass-to-charge ratios...
The paper deals with space-charge interactions in the ion population trapped Orbitrap[Formula: see text] mass analyzer where ions perform multiple quasi-harmonic oscillations axial direction. many-particle problem for interacting is mathematically complicated and its solution, even numerical, obstructed by required precision of one per million to be maintained on a large number [Formula: oscillation periods. We develop perturbation method based Bogoliubov–Krylov–Mitropolsky theory derive...
Optimizing data-independent acquisition (DIA) methods for proteomics applications often requires balancing spectral resolution and speed. Here we describe a real-time, full mass range implementation of the Phase-constrained Spectrum Deconvolution Method (ΦSDM) OrbitrapTM spectrometry that increases resolving power without increasing scan time. Comparing its performance to standard enhanced Fourier transformation (eFT) signal processing revealed increased ΦSDM is beneficial in areas high...
Reflectron-based time-of-flight analyzers rely on sub-nanosecond detector time response to achieve acceptable resolving power for low-mid mass, multiple ion peaks. With the adoption of multi-reflection analyzers, order magnitude longer folded paths relax restrictions time, allowing implementation new technologies that greatly improve dynamic range, lifetime, and detection efficiency. A system is presented, integrated into Astral analyzer, combines 10 keV post-acceleration focal plane...
The rapidly increasing adoption of high-resolution accurate-mass methods in analytical laboratories has fueled demand for instruments that combine high performance and reliability with small size greater ease-of-use. This paper presents the major design principles are driving evolution hybrid quadrupole-Orbitrap instrument architecture to enable a range applications users. These may be summarized as follows: better usage physical space access service by means reduction pumping ion optics;...