A5101722710- Lipid metabolism and biosynthesis
- Plant biochemistry and biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Photosynthetic Processes and Mechanisms
- Biofuel production and bioconversion
- Polysaccharides and Plant Cell Walls
- Enzyme Production and Characterization
- Enzyme Structure and Function
- X-ray Diffraction in Crystallography
- Legume Nitrogen Fixing Symbiosis
- Cystic Fibrosis Research Advances
- Erythrocyte Function and Pathophysiology
- Microbial Metabolites in Food Biotechnology
- Mitochondrial Function and Pathology
- Antibiotic Resistance in Bacteria
- Biochemical Acid Research Studies
- Chemical Reactions and Isotopes
- Diet, Metabolism, and Disease
- Mass Spectrometry Techniques and Applications
- Crystallization and Solubility Studies
Centre National de la Recherche Scientifique
2013-2019
Université Grenoble Alpes
2016-2019
Centre de Recherches sur les Macromolécules Végétales
2012-2019
Universidade Nova de Lisboa
2009-2011
The plant cell wall is a complex and dynamic network made mostly of cellulose, hemicelluloses, pectins. Xyloglucan, the major hemicellulosic component in Arabidopsis thaliana, biosynthesized Golgi apparatus by series glycan synthases glycosyltransferases before export to wall. A better understanding xyloglucan biosynthetic machinery will give clues toward engineering plants with improved properties or designing novel xyloglucan-based biomaterials. xyloglucan-specific α2-fucosyltransferase...
Monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) are the major lipid components of photosynthetic membranes, hence most abundant lipids in biosphere. They essential for assembly function apparatus. In Arabidopsis, first step galactolipid synthesis is catalyzed by MGDG synthase 1 (MGD1), which transfers a galactosyl residue from UDP-galactose to diacylglycerol (DAG). MGD1 monotopic protein that embedded inner envelope membrane chloroplasts. Once produced, transferred...
Members of the Burkholderia cepacia complex (BCC) are serious respiratory pathogens in immunocompromised individuals and patients with cystic fibrosis (CF). They exceptionally resistant to many antimicrobial agents have capacity spread between patients, leading a decline lung function necrotizing pneumonia. BCC members often express mucoid phenotype associated secretion exopolysaccharide (EPS) cepacian. There is much evidence supporting fact that cepacian major virulence factor BCC....
Mono- and digalactosyldiacylglycerol (MGDG DGDG) are the most abundant lipids of photosynthetic membranes (thylakoids). In Arabidopsis green tissues, MGD1 is main enzyme synthesizing MGDG. This monotopic embedded in inner envelope membrane chloroplasts. DGDG synthesis occurs outer membrane. Although suborganellar localization has been determined, it still not known how lipid/glycolipid composition influences its binding to The existence a topological relationship between "embryonic"...
Mono- and digalactosyldiacylglycerol are essential galactolipids for the biogenesis of plastids functioning photosynthetic machinery. In Arabidopsis, first step galactolipid synthesis is catalyzed by monogalactosyldiacylglycerol synthase 1 (MGD1), a monotopic protein located in inner envelope membrane chloroplasts, which transfers galactose residue from UDP-galactose to diacylglycerol (DAG). MGD1 needs anionic lipids such as phosphatidylglycerol (PG) be active, but mechanism PG activates...
Gellan gum, a commercial gelling agent produced by Sphingomonas elodea ATCC 31461, is high-value microbial exopolysaccharide. UDP-glucose dehydrogenase (UGD; EC 1.1.1.22) responsible for the NAD-dependent twofold oxidation of to UDP-glucuronic acid, one key components gellan biosynthesis. S. 31461 UGD, termed UgdG, was cloned, expressed, purified and crystallized in native SeMet-derivatized forms hexagonal tetragonal space groups, respectively; crystals diffracted X-rays 2.40 3.40 A...
The plant cell wall is a complex network of polysaccharides made up cellulose, hemicelluloses and pectins. Xyloglucan (XyG), which the main hemicellulosic component dicotyledonous plants, has attracted much attention for its role in development many industrial applications. XyG-specific fucosyltransferase (FUT1) adds fucose residue from GDP-fucose to 2-O position terminal galactosyl residues on XyG side chains. Recombinant FUT1 Arabidopsis thaliana was crystallized two different crystal...
In plant cells, galactolipids are predominant, representing up to 50% of the lipid content in photosynthetic tissues. Galactolipid synthesis is initiated by MGDG synthases (MGDs), which use UDP-galactose as a donor sugar and diacylglycerol (DAG) acceptor, form monogalactosyldiacylglycerol (MGDG). This protocol used produce recombinant Arabidopsis thaliana (A. thaliana) synthase 1 (MGD1) protein, Escherichia coli (E. coli), using two-step chromatographic purification procedure. The protein...