A5004067133- Advanced Fluorescence Microscopy Techniques
- Protein Structure and Dynamics
- Photoreceptor and optogenetics research
- Lipid Membrane Structure and Behavior
- Glycosylation and Glycoproteins Research
- Photosynthetic Processes and Mechanisms
- Advanced biosensing and bioanalysis techniques
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- Photochromic and Fluorescence Chemistry
- Enzyme Structure and Function
- Protein Interaction Studies and Fluorescence Analysis
- Nanopore and Nanochannel Transport Studies
- Enzyme Catalysis and Immobilization
- Escherichia coli research studies
- Cancer Research and Treatments
- Hemoglobin structure and function
- Force Microscopy Techniques and Applications
- Molecular spectroscopy and chirality
- DNA and Nucleic Acid Chemistry
- bioluminescence and chemiluminescence research
- Lipid metabolism and biosynthesis
- Carbohydrate Chemistry and Synthesis
- Molecular Communication and Nanonetworks
- Pharmacological Effects of Natural Compounds
Centre National de la Recherche Scientifique
2008-2019
Université Grenoble Alpes
2009-2019
Centre de Recherches sur les Macromolécules Végétales
2016-2019
University of Oxford
2011-2017
European Synchrotron Radiation Facility
2009
Karlsruhe Institute of Technology
2009
Institut de Biologie Structurale
2008-2009
Université Joseph Fourier
2008-2009
CEA Grenoble
2009
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2009
Cyan variants of green fluorescent protein are widely used as donors in Förster resonance energy transfer experiments. The popular, but modestly bright, Enhanced Fluorescent Protein (ECFP) was sequentially improved into the brighter Super 3A (SCFP3A) and mTurquoise, latter exhibiting a high-fluorescence quantum yield long mono-exponential fluorescence lifetime. Here we combine X-ray crystallography excited-state calculations to rationalize these stepwise improvements. enhancement originates...
Photoactivatable fluorescent proteins (FPs) are powerful highlighters in live cell imaging and offer perspectives for optical nanoscopy the development of biophotonic devices. Two types photoactivation currently being distinguished, reversible photoswitching between nonfluorescent forms irreversible photoconversion. Here, we have combined crystallography (in crystallo) spectroscopy to characterize Phe-173-Ser mutant tetrameric variant EosFP, named IrisFP, which incorporates both...
The epidermal growth factor receptor (EGFR) is a dimeric membrane protein that regulates key aspects of cellular function. Activation the EGFR linked to changes in conformation transmembrane (TM) domain, brought about by interactions TM helices lipid bilayer. Using an advanced computational approach combines Coarse-Grained molecular dynamics and well-tempered MetaDynamics (CG-MetaD), we characterize large-scale motions helices, simulating multiple association dissociation events between...
Enhanced cyan fluorescent protein (ECFP) and its variant Cerulean are genetically encoded fluorophores widely used as donors in FRET-based cell imaging experiments. First, we have confirmed through denaturation experiments that the double-peak spectroscopic signature of these proteins originates from indole ring chromophore. Then, to explain improvement fluorescence properties compared those ECFP, determined high-resolution crystal structures two at physiological pH performed molecular...
Supported phospholipid membrane patches stabilized on graphene surfaces have shown potential in sensor device functionalization, including biosensors and biocatalysis. Lipid dip-pen nanolithography (L-DPN) is a method useful generating supported structures that maintain lipid functionality, such as exhibiting specific interactions with protein molecules. Here, we integrated L-DPN, atomic force microscopy, coarse-grained molecular dynamics simulation methods to characterize the properties of...
Carbon nanotubes (CNTs) can penetrate the membranes of cells, offering prospects for nanomedicine but problems nanotoxicity. Molecular simulations are used to provide a systematic analysis interactions single-walled and multi-walled CNTs different radii with model lipid bilayer membrane. The allow characterization mechanism spontaneous exothermic insertion into membranes. size type CNT determine nature extent local perturbation bilayer. Single-walled shown insert via two-step initial...
Recently developed synthetic membrane pores composed of folded DNA enrich the current range natural and engineered protein nonbiogenic channels. Here we report all-atom molecular dynamics simulations a nanotube (DNT) pore scaffold to gain fundamental insight into its atomic structure, dynamics, interactions with ions water. Our multiple models DNTs that are six-duplex bundle lead coherent description. The central tube lumen adopts cylindrical shape while mouth regions at two DNT openings...
We have observed the photoactivatable fluorescent protein IrisFP in a transient dark state with near-atomic resolution. This is assigned to radical species that either relaxes ground or evolves into permanently bleached chromophore. took advantage of X-rays populate radical, which presumably forms under illumination visible light by an electron-transfer reaction triplet state. The combined X-ray diffraction and crystallo UV−vis absorption, fluorescence, Raman data reveal formation involves...
Histo-blood group epitopes are fucosylated branched oligosaccharides with well-defined conformations in solution that recognized by receptors, such as lectins from pathogens. We report here the results of a series experimental and computational endeavors revealing unusual distortion histo-blood antigens bacterial fungal lectins. The Lewis x trisaccharide adopts rigid closed conformation solution, while crystallography molecular dynamics reveal several higher energy open when bound to...
The plant cell wall is a complex and dynamic network made mostly of cellulose, hemicelluloses, pectins. Xyloglucan, the major hemicellulosic component in Arabidopsis thaliana, biosynthesized Golgi apparatus by series glycan synthases glycosyltransferases before export to wall. A better understanding xyloglucan biosynthetic machinery will give clues toward engineering plants with improved properties or designing novel xyloglucan-based biomaterials. xyloglucan-specific α2-fucosyltransferase...
Fluorescent proteins undergoing green to red photoconversion have proved be essential tools in cell biology, notably superlocalization nanoscopy. However, the exact mechanism governing photoconversion, which overall involves irreversible cleavage of protein backbone and elongation chromophore π-conjugation, remains unclear. In this paper we present a theoretical investigation reaction fluorescent EosFP, using excited-state hybrid quantum chemical molecular mechanical potentials, conjunction...
Lipases are enzymes of biotechnological importance that function at the interface formed between hydrophobic and aqueous environments. Hydrophobic interfaces can induce structural transitions in lipases result an increase enzyme activity, although detailed mechanism this process is currently not well understood for many lipases. Here, we present a multi-scale molecular dynamics simulation study how different affect conformational psychrophilic lipase M37. Our simulations show M37 able to...
Protein–carbohydrate interactions play crucial roles in biology. Understanding and modifying these is of major interest for fighting many diseases. We took a synthetic biology approach incorporated noncanonical amino acids into bacterial lectin to modulate its with carbohydrates. focused on tryptophan, which prevalent carbohydrate binding sites. The exchange the tryptophan residues analogs fluorinated at different positions resulted three distinctly variants from Ralstonia solanacearum....