A5044369377- Neurogenesis and neuroplasticity mechanisms
- Nerve injury and regeneration
- Receptor Mechanisms and Signaling
- Monoclonal and Polyclonal Antibodies Research
- Axon Guidance and Neuronal Signaling
- Neuropeptides and Animal Physiology
- Zebrafish Biomedical Research Applications
- Cellular transport and secretion
- Signaling Pathways in Disease
- Hereditary Neurological Disorders
- Cell Adhesion Molecules Research
- Mechanisms of cancer metastasis
- RNA Research and Splicing
- Microtubule and mitosis dynamics
- Neuroblastoma Research and Treatments
- Single-cell and spatial transcriptomics
- RNA Interference and Gene Delivery
- Force Microscopy Techniques and Applications
- Congenital gastrointestinal and neural anomalies
- Phagocytosis and Immune Regulation
- Pharmacogenetics and Drug Metabolism
- Biochemical and Structural Characterization
- Skin and Cellular Biology Research
Washington University in St. Louis
2015-2022
Vollum Institute
2017-2019
Oregon Health & Science University
2018-2019
University Medical Center Hamburg-Eppendorf
2015
Novo Nordisk Foundation
2015
University of Amsterdam
2015
University of Copenhagen
2015
Johannes Gutenberg University Mainz
2015
Leiden University
2015
Friedrich-Alexander-Universität Erlangen-Nürnberg
2015
Schwann cells (SCs) are essential for proper peripheral nerve development and repair, although the mechanisms regulating these processes incompletely understood. We previously showed that adhesion G protein-coupled receptor Gpr126/Adgrg6 is SC myelination. Interestingly, expression of Gpr126 maintained in adult SCs, suggestive a function mature nerve. therefore investigated role repair by studying an inducible SC-specific knock-out mouse model. Here, we show remyelination severely delayed...
Myelin is a multilamellar sheath generated by specialized glia called Schwann cells (SCs) in the peripheral nervous system (PNS), which serves to protect and insulate axons for rapid neuronal signaling. In zebrafish rodent models, we identify GPR56/ADGRG1 as conserved regulator of PNS development health. We demonstrate that, during SC development, GPR56-dependent RhoA signaling promotes timely radial sorting axons. mature PNS, GPR56 localized distinct cytoplasmic domains, required establish...
Significance Oligodendrocytes in the brain insulate neuronal axons layers of fatty myelin to facilitate fast electrical signaling. Myelin basic protein (MBP), an important component, is transported as mRNA away from cell body before being translated into protein. In zebrafish, anterograde motor kinesin transports mbp body. We now identify myelination defects zebrafish caused by a mutation retrograde complex dynein/dynactin, which normally cargos back toward However, this mutant displays...
Abstract In the central nervous system (CNS), oligodendrocytes myelinate multiple axons; in peripheral (PNS), Schwann cells (SCs) a single axon. Why are myelinating potentials of these glia so fundamentally different? Here, we find that loss Fbxw7 , an E3 ubiquitin ligase component, enhances potential SCs. mutant SCs make thicker myelin sheaths and sometimes appear to axons fashion reminiscent oligodendrocytes. Several phenotypes due dysregulation mTOR; however, remarkable ability ensheathe...
Adhesion G protein-coupled receptors (aGPCRs) are the second largest of five GPCR families and essential for a wide variety physiological processes. Zebrafish have proven to be very effective model studying biological functions aGPCRs in both developmental adult contexts. However, aGPCR repertoires not been defined any fish species, nor expression profiles tissues known. Additionally, family never extensively characterized over time-course species. Here, we report that there at least 59...
In the peripheral nervous system (PNS), specialized glial cells called Schwann produce myelin, a lipid-rich insulating sheath that surrounds axons and promotes rapid action potential propagation. During development, must undergo extensive cytoskeletal rearrangements in order to become mature, myelinating cells. The intracellular mechanisms drive cell myelination, accompanying shape changes are poorly understood. Through forward genetic screen zebrafish, we identified mutation atypical...
Abstract As forward genetic screens in zebrafish become more common, the number of mutants that cannot be identified by gross morphology or through transgenic approaches, such as many nervous system defects, has also increased. Screening for these difficult-to-visualize phenotypes demands techniques whole-mount situ hybridization (WISH) antibody staining, which require tissue fixation. To date, fixed not been amenable generating libraries whole genome sequencing (WGS). Here, we describe a...
Adhesion GPCRs are structurally identified on the basis of a large extracellular region, similar to Class B GPCR, but which is linked 7TM region by GPCR autoproteolysis-inducing (GAIN) domain [10] containing proteolysis site (GPS). The N-terminal often shares structural homology with adhesive domains (e.g. cadherins, immunolobulin, lectins) facilitating inter- and matricellular interactions leading term adhesion [104, 418]. Several receptors have been suggested function as mechanosensors...
SUMMARY Myelin insulates and protects axons in vertebrate nervous systems. In the central system (CNS), oligodendrocytes (OLs) make numerous myelin sheaths on multiple axons, whereas peripheral (PNS) myelinating Schwann cells (SCs) just one sheath a single axon. Why potentials of OLs SCs are so fundamentally different is unclear. Here, we find that loss Fbxw7, an E3 ubiquitin ligase component, enhances potential SCs. Fbxw7 mutant seen fashion reminiscent as well aberrantly large while...
Adhesion GPCRs are structurally identified on the basis of a large extracellular region, similar to Class B GPCR, but which is linked 7TM region by GPCR autoproteolysis-inducing (GAIN) domain [8] containing proteolytic site. The N-terminus often shares structural homology with adhesive domains (e.g. cadherins, immunolobulin, lectins) facilitating inter- and matricellular interactions leading term adhesion [82, 332]. Several receptors have been suggested function as mechanosensors [254, 234,...
Adhesion GPCRs are structurally identified on the basis of a large extracellular region, similar to Class B GPCR, but which is linked 7TM region by GPCR autoproteolysis-inducing (GAIN) domain [9] containing proteolytic site. The N-terminus often shares structural homology with adhesive domains (e.g. cadherins, immunolobulin, lectins) facilitating inter- and matricellular interactions leading term adhesion [101, 403]. Several receptors have been suggested function as mechanosensors [309, 280,...