A5050282636- Advanced Electron Microscopy Techniques and Applications
- Electron and X-Ray Spectroscopy Techniques
- Advanced X-ray Imaging Techniques
- Carbon Nanotubes in Composites
- Molecular Biology Techniques and Applications
- RNA modifications and cancer
- Force Microscopy Techniques and Applications
- Geophysical and Geoelectrical Methods
- Machine Learning in Materials Science
- Graphene research and applications
- Platelet Disorders and Treatments
- Blood properties and coagulation
- Genomics and Phylogenetic Studies
- Diamond and Carbon-based Materials Research
- Cell Adhesion Molecules Research
Centro Nacional de Biotecnología
2020-2024
Universidad Autónoma de Madrid
2021-2023
Consejo Superior de Investigaciones Científicas
2021
Image processing in cryogenic electron tomography (cryoET) is currently at a similar state as Single Particle Analysis (SPA) microscopy (cryoEM) was few years ago. Its data workflows are far from being well defined and the user experience still not smooth. Moreover, file formats of different software packages their associated metadata standardized, mainly since developed by groups, focusing on steps pipeline. The Scipion framework, originally for SPA (de la Rosa-Trevín et al., 2016), has...
Cryo-electron microscopy (cryoEM) has become a well established technique to elucidate the 3D structures of biological macromolecules. Projection images from thousands macromolecules that are assumed be structurally identical combined into single map representing Coulomb potential macromolecule under study. This article discusses possible caveats along image-processing path and how avoid them obtain reliable structure. Some these problems very known in community. These may referred as...
Significance Platelet activation induces reorganization of the cell and formation cellular protrusions, pseudopodia. These processes are accompanied by remodeling actin cytoskeleton platelet integrins, which mediate strong adhesion to extracellular matrix. In this work, we analyzed polarity integrin architecture in A nonuniform filaments pseudopodia indicates that these protrusions may be involved contractile acto-myosin forces. Heterogeneity conformation was found, while solely a bent...
Xmipp is an open-source software package consisting of multiple programs for processing data originating from electron microscopy and tomography, designed managed by the Biocomputing Unit Spanish National Center Biotechnology, although with contributions many other developers over world. During its 25 years existence, underwent changes updates. While there were publications related to new functionality added Xmipp, no single publication on as a since 2013. In this article, we give overview...
Abstract Image-processing pipelines require the design of complex workflows combining many different steps that bring raw acquired data to a final result with biological meaning. In image-processing domain cryo-electron microscopy single-particle analysis (cryo-EM SPA), hundreds must be performed obtain three-dimensional structure macromolecule by integrating spread over thousands micrographs containing millions copies allegedly same macromolecule. The execution such complicated demands...
Electron tomography is a technique to obtain three-dimensional structural information of samples. However, the limited by shifts occurring during acquisition that need be corrected before reconstruction process. In 2009, we proposed an approach for post-acquisition alignment tilt series images. This was marker-free, based on patch tracking and integrated in free software. Here, present improvements method make it more reliable, stable accurate. addition, modified image formation model...
Cryo-electron microscopy has become one of the most important tools in biological research to reveal structural information macromolecules at near-atomic resolution. In single-particle analysis, vitrified sample is imaged by an electron beam and detectors end microscope column produce movies that sample. These contain thousands images identical particles random orientations. The data need go through image processing workflow with multiple steps obtain final 3D reconstructed volume. goal...
Cryo-electron microscopy has become one of the most important tools in biological research to reveal structural information macromolecules at near-atomic resolution. In single-particle analysis, vitrified sample is imaged by an electron beam and detectors end microscope column produce movies that sample. These contain thousands images identical particles random orientations. The data need go through image processing workflow with multiple steps obtain final 3D reconstructed volume. goal...
Abstract The phase transition from graphite to diamond is an appealing object of study because many fundamental and also, practical reasons. out-of-plane distortions required for the are a good tool understand collective behaviour layered materials (graphene, graphite) van der Waals forces. As today, two basic processes have been successfully tested drive this transition: strong shocks high energy femtolaser excitation. They induce it by increasing either pressure or temperature on graphite....
Electron tomography is an imaging technique that allows for the elucidation of three-dimensional structural information biological specimens in a very general context, including cellular situ observations. The approach starts by collecting set images at different projection directions tilting specimen stage inside microscope. Therefore, crucial preliminary step to precisely define acquisition geometry aligning all tilt common reference. Errors introduced this will lead appearance artifacts...
Abstract Electron tomography is a technique to obtain three-dimensional structural information of samples. However, the limited by shifts occurring during acquisition that need be corrected before reconstruction process. In 2009, we proposed an approach for post-acquisition alignment tilt series images. This was marker-free, based on patch tracking and integrated in free software. Here, present improvements method make it more reliable, stable accurate. addition, modified image formation...
Electron tomography is an imaging technique that allows for the elucidation of three-dimensional structural information biological specimens in a very general context, including cellular situ observations. The approach starts by collecting set images at different projection directions tilting specimen stage inside microscope. Therefore, crucial preliminary step to precisely define acquisition geometry aligning all tilt common reference. Errors introduced this will lead appearance artifacts...