A5068160772- Nuclear Structure and Function
- RNA Research and Splicing
- Advanced Electron Microscopy Techniques and Applications
- Cellular Mechanics and Interactions
- Skin and Cellular Biology Research
- Genomics and Chromatin Dynamics
- Force Microscopy Techniques and Applications
- Cell Adhesion Molecules Research
- Photosynthetic Processes and Mechanisms
- Advanced Fluorescence Microscopy Techniques
- Platelet Disorders and Treatments
- Electron and X-Ray Spectroscopy Techniques
- Microtubule and mitosis dynamics
- Blood properties and coagulation
- RNA and protein synthesis mechanisms
- Ubiquitin and proteasome pathways
- RNA regulation and disease
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Receptor Mechanisms and Signaling
- ATP Synthase and ATPases Research
- Bacteriophages and microbial interactions
- Fungal and yeast genetics research
- Galectins and Cancer Biology
- Silk-based biomaterials and applications
Ben-Gurion University of the Negev
2014-2025
University of Zurich
2015-2024
Northwestern University
2022
Howard Hughes Medical Institute
2021
Tokyo Institute of Technology
2021
Janelia Research Campus
2021
ETH Zurich
2020
Max Planck Institute of Biochemistry
2002-2009
Max Planck Society
2002-2007
Weizmann Institute of Science
1997-2005
Electron tomography of vitrified cells is a noninvasive three-dimensional imaging technique that opens up new vistas for exploring the supramolecular organization cytoplasm. We applied this to Dictyostelium cells, focusing on actin cytoskeleton. In networks reconstructed without prior removal membranes or extraction soluble proteins, cross-linking individual microfilaments, their branching angles, and membrane attachment sites can be analyzed. At resolution 5 6 nanometers, single...
This Meeting Review describes the proceedings and conclusions from inaugural meeting of Electron Microscopy Validation Task Force organized by Unified Data Resource for 3DEM (http://www.emdatabank.org) held at Rutgers University in New Brunswick, NJ on September 28 29, 2010. At workshop, a group scientists involved collecting electron microscopy data, using data to determine three-dimensional (3DEM) density maps, building molecular models into maps explored how assess models, other that are...
Nuclear pore complexes (NPCs) are gateways for nucleocytoplasmic exchange. To analyze their structure in a close-to-life state, we studied transport-active, intact nuclei from Dictyostelium discoideum by means of cryoelectron tomography. Subvolumes the tomograms containing individual NPCs were extracted silico and subjected to three-dimensional classification averaging, whereby distinct structural states observed. The central plug/transporter (CP/T) was variable volume could occupy different...
Abstract Nuclear pore complexes (NPCs) create large conduits for cargo transport between the nucleus and cytoplasm across nuclear envelope (NE) 1–3 . These multi-megadalton structures are composed of about thirty different nucleoporins that distributed in three main substructures (the inner, cytoplasmic nucleoplasmic rings) around central channel 4–6 Here we use cryo-electron tomography on DLD-1 cells were prepared using cryo-focused-ion-beam milling to generate a structural model human NPC...
Abstract Nuclear pore complexes (NPCs) perforate the nuclear envelope and allow exchange of macromolecules between nucleus cytoplasm. To acquire a deeper understanding this transport mechanism, we analyse structure NPC scaffold permeability barrier, by reconstructing Xenopus laevis oocyte from native envelopes up to 20 Å resolution cryo-electron tomography in conjunction with subtomogram averaging. In addition resolving individual protein domains constituents, propose model for architecture...
Abstract Intermediate filaments (IFs) are integral components of the cytoskeleton. They provide cells with tissue-specific mechanical properties and involved in numerous cellular processes. Due to their intricate architecture, a 3D structure IFs has remained elusive. Here we use cryo-focused ion-beam milling, cryo-electron microscopy tomography obtain vimentin (VIFs). VIFs assemble into modular, intertwined flexible helical 40 α-helices cross-section, organized five protofibrils....
We used cryo-electron tomography in conjunction with single-particle averaging techniques to study the structures of frozen-hydrated envelope glycoprotein (Env) complexes on intact Moloney murine leukemia retrovirus particles. Cryo-electron allows 3D imaging viruses toto at a resolution sufficient locate individual macromolecules, and local abundant substantially improves resolution. The repetitive features electron tomograms is hampered by low signal-to-noise ratio anisotropic resolution,...
Numerous mutations in the human A-type lamin gene (LMNA) cause premature aging disease, progeria. Some of these are located alpha-helical central rod domain required for polymerization nuclear lamins into higher order structures. Patient cells with a mutation this domain, 433G>A (E145K) show severely lobulated nuclei, separation A- and B-type lamins, alterations pericentric heterochromatin, abnormally clustered centromeres, mislocalized telomeres. The induction lobulations clustering...
The architecture of a signaling hub Adenylyl cyclases (ACs) respond to variety inputs generate the molecule cyclic adenosine monophosphate. ACs are regulated by G proteins, which activated upstream receptors. Qi et al. determined structure bovine membrane AC9 bound an protein αs subunit cryo–electron microscopy at 3.4-angstrom resolution. provides full AC9, including helical domain that connects transmembrane and catalytic domains. model reveals how domains interact regulate enzymatic...
Bacteria display an array of contact-dependent interaction systems that have evolved to facilitate direct cell-to-cell communication. We previously identified a mode bacterial communication mediated by nanotubes bridging neighboring cells. Here, we elucidate nanotube architecture, dynamics, and molecular components. Utilizing Bacillus subtilis as model organism, found at low cell density, exhibit remarkable complexity, existing both intercellular tubes extending tubes, with the latter...
Most systemic viral gene therapies have been limited by sequestration and degradation of virions, innate adaptive immunity, silencing therapeutic genes within the target cells. Here we engineer a high-affinity protein coat, shielding most commonly used vector in clinical therapy, human adenovirus type 5. Using electron microscopy crystallography demonstrate massive coverage virion surface through hexon-shielding scFv fragment, trimerized to exploit hexon symmetry gain avidity. The shield...