A5049607374- Connexins and lens biology
- Yersinia bacterium, plague, ectoparasites research
- Nicotinic Acetylcholine Receptors Study
- DNA and Nucleic Acid Chemistry
- Hedgehog Signaling Pathway Studies
- Heat shock proteins research
- Amino Acid Enzymes and Metabolism
- Heat Transfer and Boiling Studies
- Heat Transfer and Optimization
- Alzheimer's disease research and treatments
- Hydrocarbon exploration and reservoir analysis
- Porphyrin and Phthalocyanine Chemistry
- Metabolomics and Mass Spectrometry Studies
- Receptor Mechanisms and Signaling
- Protein Kinase Regulation and GTPase Signaling
- Sulfur Compounds in Biology
- Kruppel-like factors research
- Biochemical effects in animals
- Refrigeration and Air Conditioning Technologies
- Genomics and Chromatin Dynamics
- Prion Diseases and Protein Misfolding
- Natural product bioactivities and synthesis
- Hydraulic Fracturing and Reservoir Analysis
- Theoretical and Computational Physics
- Heat transfer and supercritical fluids
MRC Laboratory of Molecular Biology
2023-2025
ETH Zurich
2019-2024
Paul Scherrer Institute
2019-2024
Northwestern University
2022-2024
Shanxi Academy of Medical Sciences
2023-2024
Shanxi Medical University
2023-2024
Shandong Province Animal Husbandry and Veterinary Bureau
2023-2024
Medical Research Council
2023-2024
Affiliated Hospital of Shandong University of Traditional Chinese Medicine
2024
Peking University
2012-2024
The architecture of a signaling hub Adenylyl cyclases (ACs) respond to variety inputs generate the molecule cyclic adenosine monophosphate. ACs are regulated by G proteins, which activated upstream receptors. Qi et al. determined structure bovine membrane AC9 bound an protein αs subunit cryo–electron microscopy at 3.4-angstrom resolution. provides full AC9, including helical domain that connects transmembrane and catalytic domains. model reveals how domains interact regulate enzymatic...
Both connate water and the injected through hydraulic fracturing can coexist with methane inside shale nanopores where two-phase flow possibly occurs. Few studies have been pertaining to of in reservoirs at nanoscale. In this work, molecular dynamics simulations are employed investigate slit-shaped silica hydrophilic surfaces. A sandwich structure film–methane–water film or a gas bubble wrapped bridge exists nanopore because wets Darcy's law breaks down for single-phase slippage near For...
Abstract Organic cation transporters (OCTs) facilitate the translocation of catecholamines, drugs and xenobiotics across plasma membrane in various tissues throughout human body. OCT3 plays a key role low-affinity, high-capacity uptake monoamines most including heart, brain liver. Its deregulation diseases. Despite its importance, structural basis function inhibition has remained enigmatic. Here we describe cryo-EM structure at 3.2 Å resolution. Structures bound to two inhibitors,...
Subacute sclerosing panencephalitis (SSPE) occurs in some individuals after measles infection, following a symptom-free period of several years. It resembles chronic traumatic encephalopathy (CTE), which happens repetitive head impacts or exposure to blast waves, period. As CTE, the neurofibrillary changes SSPE are concentrated superficial cortical layers. Here we used electron cryo-microscopy (cryo-EM) tau filaments from two cases show that folds and CTE identical. Two types were each made...
The amyotrophic lateral sclerosis/parkinsonism-dementia complex (ALS/PDC) of the island Guam and Kii peninsula Japan is a fatal neurodegenerative disease unknown cause that characterized by presence abundant filamentous tau inclusions in brains spinal cords. Here, we used electron cryo-microscopy to determine structures filaments from cerebral cortex three cases ALS/PDC eight Kii, as well cord two cases. Tau had chronic traumatic encephalopathy (CTE) fold, with variable amounts Type I II...
Frontotemporal dementia (FTD) and Alzheimer's disease (AD) are the most common forms of early-onset dementia. Unlike AD, FTD begins with behavioral changes before development cognitive impairment. Dominantly inherited mutations in MAPT, microtubule-associated protein tau gene, give rise to cases parkinsonism linked chromosome 17. These individuals develop abundant filamentous inclusions brain cells absence β-amyloid deposits. Here, we used cryo-electron microscopy determine structures...
Adenine deoxynucleosides, such as 2-chloro-2′-deoxyadenosine (2CdA) induce apoptosis in quiescent lymphocytes, and are thus useful drugs for the treatment of indolent lymphoproliferative diseases. However, it has remained puzzling why deoxyadenosine its analogs toxic to a cell that is not undergoing replicative DNA synthesis. The present experiments demonstrate 5′-triphosphate metabolite 2CdA (2CdA-5′-triphosphate), similar dATP, can cooperate with cytochrome c Apaf-1 activate caspase-3 free...
Cryo-EM structure of PTCH1-ShhN C24II complex reveals multiple sterol binding sites and a possible translocation pathway.
Abstract Adenylyl cyclase 9 (AC9) is a membrane-bound enzyme that converts ATP into cAMP. The weakly activated by forskolin, fully the G protein Gαs subunit and autoinhibited AC9 C-terminus. Although our recent structural studies of AC9-Gαs complex provided framework for understanding autoinhibition, conformational changes undergoes in response to activator binding remains poorly understood. Here, we present cryo-EM structures several distinct states: (i) bound nucleotide inhibitor MANT-GTP,...
Gap junction channels (GJCs) mediate intercellular communication by connecting two neighbouring cells and enabling direct exchange of ions small molecules. Cell coupling via connexin-43 (Cx43) GJCs is important in a wide range cellular processes health disease (Churko Laird, 2013; Liang et al., 2020; Poelzing Rosenbaum, 2004), yet the structural basis Cx43 function regulation has not been determined until now. Here, we describe structure human GJC solved cryo-EM single particle analysis at...
Gap junction channels (GJCs) mediate intercellular communication by connecting two neighbouring cells and enabling direct exchange of ions small molecules. Cell coupling via connexin-43 (Cx43) GJCs is important in a wide range cellular processes health disease (Churko Laird, 2013; Liang et al., 2020; Poelzing Rosenbaum, 2004), yet the structural basis Cx43 function regulation has not been determined until now. Here, we describe structure human GJC solved cryo-EM single particle analysis at...
Frontotemporal dementia (FTD) and Alzheimer's disease are the most common forms of early-onset dementia. Dominantly inherited mutations in
The Hedgehog (Hh) signaling pathway controls embryonic development and adult tissue homeostasis in multicellular organisms. In Drosophila melanogaster, the is primed by secretion of a dually lipid-modified morphogen, Hh, process dependent on membrane-integral protein Dispatched. Although Dispatched critical component pathway, structural basis its activity has, so far, not been described. Here, we describe cryo-electron microscopy structure D. melanogaster at 3.2-Å resolution. ectodomains...
Abstract Background Body weight and size are important economic traits in chickens. While many growth-related quantitative trait loci (QTLs) candidate genes have been identified, further research is needed to confirm characterize these findings. In this study, we investigate genetic genomic markers associated with chicken body size. This study provides new insights into potential for selection breeding strategies improve meat production Methods We performed whole-genome resequencing of...
In myelinating Schwann cells, connection between myelin layers is mediated by gap junction channels (GJCs) formed docked connexin 32 (Cx32) hemichannels (HCs). Mutations in Cx32 cause the X-linked Charcot-Marie-Tooth disease (CMT1X), a degenerative neuropathy without cure. A molecular link dysfunction and CMT1X pathogenesis still missing. Here, we describe high-resolution cryo-electron microscopy (cryo-EM) structures of GJC HC, along with two CMT1X-linked mutants, W3S R22G. While wild-type...