A5002206741- Plant tissue culture and regeneration
- Seed Germination and Physiology
- Fern and Epiphyte Biology
- Plant and animal studies
- Plant Pathogens and Fungal Diseases
- Cocoa and Sweet Potato Agronomy
- Botany and Plant Ecology Studies
- Ecology and Vegetation Dynamics Studies
- Plant Reproductive Biology
- Lichen and fungal ecology
- Turfgrass Adaptation and Management
- Bryophyte Studies and Records
- Botany, Ecology, and Taxonomy Studies
- Reproductive Health and Technologies
- Seedling growth and survival studies
- Flowering Plant Growth and Cultivation
- Plant Genetic and Mutation Studies
- Reproductive Biology and Fertility
- Mycorrhizal Fungi and Plant Interactions
- Plant Taxonomy and Phylogenetics
- Plant Molecular Biology Research
- Plant Pathogens and Resistance
- Rangeland and Wildlife Management
- Transgenic Plants and Applications
- Food Chemistry and Fat Analysis
Cincinnati Zoo and Botanical Garden
2014-2024
University of Cincinnati
1981-2022
University of Florida
1982
Purdue University West Lafayette
1979-1981
Societal Impact Statement Eroding plant diversity has serious implications for the well‐being of humanity and our planet. Conserving plants ex situ requires technologies that are rapidly advancing readily accessible. The alarming loss habitats spawned global investment in focus on either conventional freezer storage, which exploit seed adaptations to survive drying, or cryogenic platforms, ensure long‐term survival germplasm is not amenable methods. Increasing evidence survives decades...
The multiple, complex risks to plant species survival are increasing, and conservation strategies that include ex situ as well in approaches needed now more than ever. Seed banking (drying at c.15% relative humidity then storing −20 °C) is the most efficient effective method for long-term a majority of species. However, some not amenable this practice, such have been labeled exceptional. Critical limitations conventional seed include: 1) unavailability sufficient storage; 2) seeds completely...
With two in five plant species facing extinction, diversity loss demands action that integrates ex situ with conservation approaches. While seed banks has been widely adopted, this technology cannot be applied to exceptional species, which require alternative We propose a new conceptual framework, resulting the creation of global working Exceptional Status List. Using semi-automated method evaluate over 23,000 plants, 775 were identified as exceptional, <3% those predicted exceptional. These...
Abstract Cultured immature sexual embryos of Theobroma cacao proliferated asexual embryos, but embryogenesis was not observed from leaf, pericarp, or ovule tissues. Cotyledons immature, unpigmented (2.5 to 10 mm long) were highly embryognie, while larger more mature, pigmented cotyledons initiated roots as their only form organized development. Embryogenesis occurred in light and darkness when a basal medium supplemented with coconut water 1.5 mg/liter NAA. Proliferated recultured liquid continued
For the majority of plant species conservation concern, seed banking and traditional propagation methods are most efficient ways meeting ex situ recovery goals Global Strategy for Plant Conservation (GSPC) Target 8. However, there estimated to be 5000 or more endangered which these will not adequate tools. These "exceptional" those with recalcitrant seeds that produce few no seeds. In vitro can provide alternative procedures propagating preserving germplasm in long term species. Research at...
Cryopreservation of nonseed plant tissues can provide a method for preserving genetic diversity ex situ when seeds or spores cannot be adapted to traditional methods preservation. Shoot tips, somatic embryos, and gametophytes used as propagules long-term storage in liquid nitrogen. Tissue cryopreservation is particularly important dealing with "exceptional species," i.e., species that have unbankable are producing few no seeds. In vitro applied collecting establishing cultures source tissue...
Abstract The conservation of threatened exceptional plants, which cannot be conserved by seed banking, requires in vitro technologies for many the approaches needed their long-term ex situ conservation. This study evaluated current plant literature, as represented Web Science, to determine its taxonomic overlap with families and genera 775 species currently listed exceptional. Science was searched using terms micropropagation, somatic embryogenesis, zygotic embryo, cryopreservation, target...
Asexual embryos of Theobroma cacao L. cultured in vitro grew to a size and fresh weight equal mature zygotic vivo when liquid medium on rollodrum apparatus. Changes the fatty acid composition asexual embryos, similar those which occur embryo during normal maturation, were stimulated as sucrose concentration was increased an optimum at 27% (w/v). As increased, there shift from predominantly polyunsaturated saturated monounsaturated acids characterized by increase mole percents stearic (18:0)...
Abstract Premise The effective ex situ conservation of exceptional plants, whether in living collections or cryo‐collections, requires more resources than the other species. Because their expertise with rare botanical gardens are well positioned to lead this effort, but a well‐developed strategy clear understanding needed. Methods Grant funding was obtained from Institute Museum and Library Services support three‐year project on cryobanking, provide smaller grants 10 for one‐year projects...
Thirty-three species of ferns with nonchlorophyllous spores and five chlorophyllous were studied in regard to their ability survive exposure liquid nitrogen (LN).Air dried showed no inhibition germination after LN when planted on soil or growth medium.Spores three that stored for 75 months either at 4°C, -20°C» decrease viability over time, four other maintained successfully 52 LN, Fresh air dried, silica gel, prepared the encapsulation dehydration procedure also good survival through...
Cryopreservation is increasingly important as a conservation tool, particularly for threatened exceptional species. The goal of this study was to investigate the current knowledge plant cryopreservation through search literature in Web Science and align that with 775 species currently identified on Working List Exceptional Plants. While there good foundation research, economically species, are significant gaps research families contain largest numbers known including Dipterocarpaceae,...