A5054853721- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Bacteriophages and microbial interactions
- Molecular Biology Techniques and Applications
- COVID-19 diagnosis using AI
Dalhousie University
2020-2024
A simple passive sampling protocol using cheesecloth and electronegative filters coupled with a Tween®20-based elution technique provided reliable detection of SARS-CoV-2 in wastewater at targeted locations region low COVID-19 prevalence.
In developing an effective monitoring program for the wastewater surveillance of SARS-CoV-2 ribonucleic acid (RNA), importance sampling methodology is paramount. Passive has been shown to be tool detect RNA in wastewater. However, adsorption characteristics on passive material are not well-understood, which further obscures relationship between and community infection. this work, kinetics equilibrium were evaluated using batch-adsorption experiments heat-inactivated (HI-SCV-2)...
Based on recent studies, passive sampling is a promising method for detecting SARS-CoV-2 in wastewater surveillance (WWS) applications. Passive has many advantages over conventional approaches. However, the potential benefits of are also coupled with apparent limitations. We established technique severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using electronegative filters. Though, it was evident that adsorption capacity filters constrained their use. This work intends to...
We demonstrate the application of a rapid pre-concentration protocol and magnetic bead-based RNA extraction method for detection SARS-CoV-2 from raw wastewater without need extensive sample treatment.
Human viruses pose a significant health risk in freshwater environments, but current monitoring methods are inadequate for detecting viral presence efficiently. We evaluated novel passive in-situ concentration method using granular activated carbon (GAC). This study detected and quantified eight enteric non-enteric, pathogenic recreational lake paired grab GAC samples. The results found that sampling had higher detection rate all compared to samples, with adenovirus be the most prevalent...
Wastewater-based surveillance methods have been implemented in several countries as a tool for monitoring SARS-CoV-2 at community scale. A variety of used concentrating, extracting, and detecting the virus, with no clear consensus on most effective approach. In this note, we report preliminary findings from study that is tracking wastewater Halifax, Nova Scotia, specific focus use four reverse transcriptase quantitative PCR (RT-qPCR) assays virus wastewater. We were able to detect samples...
Abstract The use of magnetic beads in the extraction nucleic acids from wastewater is presented as an approach to simplify techniques for detection SARS-CoV-2 viral fragments wastewater. In particular, this paper describes development and optimization a direct method extracting RNA municipal using beads. recovery efficiency Accuplex Positive Reference Material (ASCV-2) was examined. Method factors assessed were sample volume, concentration bead mix, elution temperature, water matrix...
Developing a simple and cost-effective wastewater concentration method using powdered activated charcoal sodium alginate (PAC-NaA) hydrogel beads, enhanced for capturing viruses.
This study evaluates the efficacy of wastewater surveillance and passive sampling techniques for early detection SARS-CoV-2 Omicron variant in a university setting Halifax, Canada.
The COVID-19 pandemic has underscored the need for effective viral tracking in aqueous environments, particularly non-enteric viruses. Despite advances wastewater monitoring, surveillance of viruses freshwater remains limited due to traditional sampling challenges. This study refines GAC-based passive protocols by determining optimal extraction and elution methods enhancing recovery nucleic acids freshwater. Three commercially available total acid (TNA) kits four buffers were assessed their...
Based on recent studies, passive sampling is a promising method for detecting SARS-CoV-2 in wastewater surveillance (WWS) applications. Passive has many advantages over conventional approaches. However, the potential benefits of are also coupled with apparent limitations. We established technique severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using electronegative filters. Though, it was evident that adsorption capacity filters constrained their use. This work intends to...
Abstract Human viruses pose a significant health risk in freshwater environments, but current monitoring methods are inadequate for detecting viral presence efficiently. We evaluated novel passive in-situ concentration method using granular activated carbon (GAC). This study detected and quantified eight enteric non-enteric pathogenic recreational lake paired grab GAC samples. Results found that sampling had higher detection rate all compared to samples, with adenovirus be the most prevalent...