A5053948161- Neonatal Respiratory Health Research
- T-cell and B-cell Immunology
- Medical Imaging and Pathology Studies
- Immune Cell Function and Interaction
- Cancer-related gene regulation
- Pulmonary Hypertension Research and Treatments
- Wnt/β-catenin signaling in development and cancer
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Tracheal and airway disorders
- Congenital Diaphragmatic Hernia Studies
- Respiratory Support and Mechanisms
- Estrogen and related hormone effects
- NF-κB Signaling Pathways
- Neuroscience of respiration and sleep
- Neuroscience and Neuropharmacology Research
- RNA modifications and cancer
- Connective Tissue Growth Factor Research
- Heat shock proteins research
- Occupational exposure and asthma
- Hormonal Regulation and Hypertension
- Diabetes and associated disorders
- Sphingolipid Metabolism and Signaling
- Cancer Cells and Metastasis
University of Pecs
2003-2019
University of Birmingham
2012-2016
Centre for Inflammation Research
2016
Pharmaceutical Biotechnology (Czechia)
2015
Queen Elizabeth Hospital Birmingham
2014
<h3>Rationale</h3> Vitamin D deficiency has been implicated as a pathogenic factor in sepsis and intensive therapy unit mortality but not assessed risk for acute respiratory distress syndrome (ARDS). Causality of these associations never demonstrated. <h3>Objectives</h3> To determine if ARDS is associated with vitamin clinical setting to experimental models influences its severity. <h3>Methods</h3> Human, murine vitro primary alveolar epithelial cell work were included this study....
Type 2 alveolar epithelial cells (AT2s) behave as stem and show clonal proliferation upon injury followed by trans-differentiation (TD) into 1 (AT1s). In the present study we identified signaling pathways involved in physiological AT2-to-AT1 TD process. AT2 can be isolated from human lungs cultured vitro where they undergo AT1s. process using Affymetrix microarray, qRT-PCR, fluorescence microscopy, an lung aggregate culture. microarray revealed Wnt to play a crucial role Wnt7a was ligand...
Age-associated thymic involution has considerable physiological impact by inhibiting de novo T-cell selection. This impaired production leads to weakened immune responses. Yet the molecular mechanisms of stromal adipose are not clear. Age-related alterations also occur in murine thymus providing an excellent model system. In present work structural and changes stroma were investigated during aging. We show that epithelial senescence correlates with significant destruction network followed...
The majority of lung cancers (LC) belong to the non-small cell carcinoma (NSCLC) type. two main NSCLC sub-types, namely adenocarcinoma (AC) and squamous (SCC), respond differently therapy. Whereas link between cigarette smoke cancer risk is well established, relevance non-canonical Wnt pathway up-regulation detected in SCC remains poorly understood. present study was undertaken investigate further molecular events canonical signalling during development. A total 20 AC samples with matched...
Glucocorticoid receptor (GR) signaling plays an important role in the selection and apoptosis of thymocytes. Besides nuclear translocation, mitochondrial translocation ligand-bound GR lymphoid cells was also shown, which might determine glucocorticoid (GC)-induced sensitivity. In present work, we followed ligand-induced trafficking CD4+CD8+ double-positive (DP) Using confocal microscopy, found that upon short-term vitro GC analog [dexamethasone (DX)] treatment, translocates into mitochondria...
In the aging lung, lung capacity decreases even in absence of diseases. The progenitor cells distal alveolar type II (ATII), are essential for repair gas-exchange surface. Surfactant protein production and survival ATII supported by lipofibroblasts that peroxisome proliferator-activated receptor gamma (PPARγ)-dependent special cell pulmonary tissue. PPARγ levels directly regulated Wnt molecules; therefore, changes microenvironment have close control over maintenance lung. process is...
Members of the Wnt family secreted glyco-lipo-proteins affect intrathymic T-cell development and are abundantly by thymic epithelial cells (TECs) that create specific microenvironment for thymocytes to develop into mature T-cells. During ageing, expression declines allowing adipoid involution epithelium leading reduced naïve output. The protein kinase C (PKC) serine-threonine kinases is involved in numerous intracellular biochemical processes, including signal transduction. In present study,...
CD248 or Endosialin is a transmembrane molecule expressed in stromal cells binding to extracellular matrix (ECM) components. It has been previously implicated kidney fibrosis, rheumatoid arthritis as well tumour-stromal interactions. This study investigates the role of pathogenesis fibrotic diseases Idiopathic Pulmonary Fibrosis (IPF). quantitative immunohistochemistry (IHC) was performed on lung samples from 22 IPF patients and its expression assayed cultured pulmonary fibroblasts...
Therapy that promotes epithelial repair whilst protecting against fibroproliferation is critical for restoring lung function in acute and chronic respiratory diseases. Primary human alveolar type II cells were used to model the effects of lipoxin A 4 vitro upon wound repair, proliferation, apoptosis transdifferention. Effects primary fibroblast collagen production, myofibroblast differentiation also assessed. Lipoxin promoted cell blocked negative soluble Fas ligand/tumour necrosis factor α...
Zeta-chain-associated protein kinase of 70kDa (ZAP-70) is a key regulator in the early steps TCR signaling but some aspects its fine regulation are still unclear. From 31 tyrosine (Y) residues, 11 phosphorylation sites have been identified, with activator (Y315 and Y493) or inhibitory (Y292 Y492) others unknown function (Y069, Y126 Y178). In our present work, we aimed to elucidate role different Y residues ZAP-70, especially those function, calcium autoregulation kinase. ZAP-70-deficient...
<h3>Introduction</h3> Effective clearance of apoptotic cells by macrophages, termed efferocytosis, is a pre-requisite for successful resolution inflammation. High mobility group box protein 1 (HMGB1), an alarmin that may promote inflammation as well suppress phagocytosis. Lipoxin A<sub>4</sub>, represents one unique class lipid mediators possess wide spectrum anti-inflammatory and pro-resolution actions. We hypothesised lipoxin A<sub>4</sub> both apoptosis in neutrophils, stimulate...
<h3></h3> Idiopathic pulmonary fibrosis (IPF) has a complex pathophysiology withepithelial-mesenchymal transition (EMT) thought to be important the pathogenesis of fibrotic lesions. CD248 is membrane bound receptor that collagen and lectins as ligands stromal cell marker, whose expression up-regulated post-natally during tissue inflammation andre-modelling. A role emerging in kidney fibrosis, but its function lung unknown. We hypothesised mesenchymal marker IPF severity contributes...
The putative progenitor alveolar epithelial type II (ATII) cells restore barrier integrity by transdifferentiating into I (ATI) cells. We investigated to see whether inhibition of histone deacetylases (HDAC) could reverse mesenchymal (EMT) like intermediate phenotype during differentiation ATII <b>Methods:</b> Primary human in monolayer or co-cultured with fibroblasts were treated 5µM HDAC inhibitor (HDACi) MS-275 (MS) and 10ng/ml TGFβ assess molecular targets. <b>Results:</b> MS upregulated...