A5057995136- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Mitochondrial Function and Pathology
- Nuclear Structure and Function
- Carcinogens and Genotoxicity Assessment
- Microtubule and mitosis dynamics
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- Nanoplatforms for cancer theranostics
- ATP Synthase and ATPases Research
- Virus-based gene therapy research
- Advanced Nanomaterials in Catalysis
- Cell death mechanisms and regulation
University of Puerto Rico at Ponce
2025
Cornell University
2021-2022
The phosphatidylinositol 3′ kinase (PI3K)‐related ATR is crucial for mammalian meiosis. promotes meiotic progression by coordinating key events in DNA repair, sex chromosome inactivation (MSCI), and checkpoint-dependent quality control during prophase I. Despite its central roles meiosis, the ATR-dependent signaling network remains largely unknown. Here, we used phosphoproteomics to define testes from mice following chemical genetic ablation of signaling. Quantitative analysis...
Lung cancer remains the most common malignancy independent of sex. Here, we focused on unraveling molecular mechanisms CaS nanoclusters inducing cytotoxicity by investigating DNA damage, cell cycle, oxidative stress, and cellular repair in non-small-cell lung carcinoma (NSCLC) cells compared to healthy fibroblasts. Our previous studies have demonstrated therapeutic potential calcium sulfide (CaS) nanostructures skin breast models, leading a significant reduction proliferation. However, how...
DNA damage response mechanisms have meiotic roles that ensure successful gamete formation. While completion of double-strand break (DSB) repair requires the canonical RAD9A-RAD1-HUS1 (9A-1-1) complex, mammalian meiocytes also express RAD9A and HUS1 paralogs, RAD9B HUS1B, predicted to form alternative 9-1-1 complexes. The RAD1 subunit is shared by all complexes localizes chromosomes even in absence RAD9A. Here, we report testis-specific disruption mice resulted impaired DSB repair, germ cell...
Abstract During mammalian meiosis, the ATR kinase plays crucial roles in coordination of DNA repair, meiotic sex chromosome inactivation and checkpoint signaling. Despite importance signaling network remains largely unknown. Here we defined during prophase I mice. Quantitative analysis phosphoproteomes obtained after genetic ablation ATR-activating 9-1-1 complex or chemical inhibition revealed over 12,000 phosphorylation sites, which 863 sites were dependent on both ATR. 9-1-1-dependent was...
ABSTRACT DNA damage response mechanisms have meiotic roles that ensure successful gamete formation. While completion of double-strand break (DSB) repair requires the canonical RAD9A-RAD1-HUS1 (9A-1-1) complex, mammalian meiocytes also express RAD9A and HUS1 paralogs, RAD9B HUS1B, predicted to form alternative 9-1-1 complexes. The RAD1 subunit is shared by all complexes localizes chromosomes even in absence RAD9A. Here we report testis-specific disruption resulted impaired DSB repair, germ...