A5083588685- Pulmonary Hypertension Research and Treatments
- Pneumocystis jirovecii pneumonia detection and treatment
- HIV-related health complications and treatments
- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Vascular Anomalies and Treatments
- Eosinophilic Disorders and Syndromes
- Peptidase Inhibition and Analysis
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Immunotherapy and Immune Responses
- Bacteriophages and microbial interactions
- Transplantation: Methods and Outcomes
- Chromosomal and Genetic Variations
- Plant Virus Research Studies
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- SARS-CoV-2 and COVID-19 Research
- Epigenetics and DNA Methylation
- Diversity and Career in Medicine
- Viral-associated cancers and disorders
- Wnt/β-catenin signaling in development and cancer
- Infection Control and Ventilation
- COVID-19 diagnosis using AI
- Autophagy in Disease and Therapy
- Genetics and Neurodevelopmental Disorders
Texas Tech University
2017-2025
Texas Tech University Health Sciences Center
2017-2025
University of Colorado Anschutz Medical Campus
2009-2022
Clinical Research Puerto Rico
2020
University of Puerto Rico at Ponce
2020
Creative Commons
2020
Ponce Health Sciences University
2004-2020
Weatherford College
2020
Puerto Rico Science, Technology & Research Trust
2020
University of Colorado Denver
2009-2016
With effective antiretroviral therapy (ART), cardiovascular diseases (CVD) are emerging as a major cause of morbidity and death in the aging HIV-infected population. To address whether HIV-Nef, viral protein produced infected cells even when virus production is halted by ART, can lead to endothelial activation dysfunction, we tested Nef transfer activity cells. We demonstrated that essential for cell activating effects Jurkat direct contact with endothelium. In addition, found sufficient...
Section:ChooseTop of pageAbstract <<CLINICAL PRESENTATION, DI...PATHOGENESIS OF HIV-ASSOC...HIV NEF SEQUENCES: BIOMAR...CONCLUSIONSReferencesCITING ARTICLES
Macroautophagy/autophagy is an auto-digestive pro-survival pathway activated in response to stress target cargo for lysosomal degradation. In recent years, autophagy has become prominent as innate antiviral defense mechanism through multiple processes, such targeting virions and viral components elimination. These exciting findings have encouraged studies on the ability of restrict HIV. However, role HIV infection remains unclear. Whereas some reports indicate that detrimental HIV, others...
Golgi dysfunction has been previously investigated as a mechanism involved in monocrotaline-induced pulmonary hypertension (PAH). In the present study, we addressed whether might occur vascular cells idiopathic PAH (IPAH) and there be causal relationship between trafficking vasculopathies of PAH. Quantitative immunostaining for tethers giantin p115 on human lung tissue from patients with IPAH (n = 6) compared controls demonstrated marked cytoplasmic dispersal giantin- p115-bearing vesicular...
Severe pulmonary hypertension (PH) associated with vascular remodeling is a long-term complication of HIV infection (HIV-PH) affecting 1/200 infected individuals vs. 1/200,000 frequency in the uninfected population. Factors accounting for increased PH susceptibility HIV-infected are unknown. Rhesus macaques chimeric SHIVnef virions but not SIV display PH-like suggesting that HIV-Nef PH; these monkeys showed changes nef sequences correlated pathogenesis after passage vivo. We further examined...
// Isabel Castro-Piedras 1, * , Monica Sharma Meghan den Bakker 1 Deborah Molehin Edgar G. Martinez David Vartak Wendy M. Pruitt Jena Deitrick Sharilyn Almodovar and Kevin Department of Immunology & Molecular Microbiology, Texas Tech University Health Sciences Center, Lubbock, TX, USA These authors have equally contributed to this work Correspondence to: Pruitt, email: kevin.pruitt@ttuhsc.edu Keywords: aromatase; CYP19A1; dishevelled; DVL; breast cancer Received: August 19, 2018 Accepted:...
Lung cancer remains the most common malignancy independent of sex. Here, we focused on unraveling molecular mechanisms CaS nanoclusters inducing cytotoxicity by investigating DNA damage, cell cycle, oxidative stress, and cellular repair in non-small-cell lung carcinoma (NSCLC) cells compared to healthy fibroblasts. Our previous studies have demonstrated therapeutic potential calcium sulfide (CaS) nanostructures skin breast models, leading a significant reduction proliferation. However, how...
The viral kinetics of HERV-K in HIV-1-infected patients receiving highly active antiretroviral therapy (HAART) is not unknown. kinetic modeling may provide insight into factors altering the effectiveness HAART suppressing HIV-1 burden. We conducted a longitudinal study measuring RNA titers four with successful HIV-1-suppressive and six undergoing failure. were usually undetectable HAART, when detected, remained below 5000 copies/ml. On other hand, was consistently detected who failed to...
Point-of-care diagnostic devices that are rapid and reliable remain as an unmet need highlighted by the coronavirus disease (COVID-19) pandemic crisis. The second/third wave of virus spread in various parts world combined with new evidence re-infections inadequate healthcare facilities demand increased testing rate to diagnose COVID-19 at its core. Although traditional molecular tests have served this purpose, there been shortage reagents other supplies frontlines. This calls for novel...
People living with HIV (PLWH) face a growing burden of chronic diseases, owing to the combinations aging, environmental triggers, lifestyle choices, and virus-induced inflammation. The rising incidence pulmonary vascular diseases represents major concern for PLWH. study HIV-associated complications ideally requires strong understanding cell biology pathogenesis at molecular level effective applications in infectious medicine. Active infection and/or proteins disturb delicate balance between...
Although reduced bioavailability of nitric oxide (NO) has been implicated in the pathogenesis pulmonary arterial hypertension (PAH), its consequences on organellar structure and function within vascular cells is largely unexplored. We investigated effect NO Golgi apparatus as assayed by giantin or GM130 immunofluorescence human endothelial (HPAECs) smooth muscle (HPASMCs) cells, bovine PAECs, EA.hy926 cells. was also tissue sections lesions idiopathic PAH (IPAH) macaques infected with a...
Section:ChooseTop of pageAbstract <<Topics DiscussedRole the Academic Rese...Life beyond a Research Pr...Facilitating Career Devel...Effectiveness Current ...SynopsisReferencesCITING ARTICLES
The COVID-19 pandemic triggered the widespread use and need for respirators face masks healthcare workers public. In this study, several generally available mask designs were fit tested, their materials evaluated filtration efficiency using 250 nm polystyrene latex particles. Efficiency testing was performed 2 0.5 h at low (2.6 L min–1) high (7.4 airflows, respectively, ~17.4 cm2 material area. As expected, all N95 KN95 passed test, showed efficiencies > 95% entire experiment both airflows....
Abstract Leveraging the potential of virtual platforms in post‐COVID‐19 era, Infection and Pulmonary Vascular Diseases Consortium (iPVDc), with support Research Institute (PVRI), launched a globally accessible educational program to highlight top‐notch research on inflammation infectious diseases affecting lung vasculature. This innovative series has already successfully brought together distinguished investigators across five continents – Asia, Europe, South North America, Africa. Moreover,...
Abstract Background Dishevelled paralogs (DVL1, 2, 3) are key mediators of Wnt pathway playing a role in constitutive oncogenic signaling influencing the tumor microenvironment. While previous studies showed correlation β-catenin with T cell gene expression, little is known about DVL2 modulating immunity. This study aimed to uncover novel interaction between and HER2-positive (HER2+) breast cancer (BC) regulating immunity disease progression. Methods loss function were performed or without...
Fatal pulmonary arterial hypertension (PAH) affects HIV-infected individuals at significantly higher frequencies. We previously showed plexiform-like lesions characterized by recanalized lumenal obliteration, intimal disruption, medial hypertrophy, and thrombosis consistent with PAH in rhesus macaques infected chimeric SHIVnef but not the parental SIVmac239, suggesting that Nef is implicated pathophysiology of HIV-PAH. However, current literature on non-human primates as animal models for...
DVL proteins are central mediators of the Wnt pathway and relay complex input signals into different branches signaling network. However, molecular mechanism(s) that regulate DVL-mediated still remains unclear. Here, for first time, we elucidate functional significance three DVL-1 lysines (K/Lys) which subject to post-translational acetylation. We demonstrate K34 Lys residue in DIX domain regulates subcellular localization β-catenin, thereby influencing downstream target gene expression....
Inflammatory pain is caused by tissue hypersensitization and a component of rheumatic diseases, frequently causing chronic pain. Current guidelines utilize multimodal approach to sociocultural changes have renewed interest in cannabinoid use, particularly cannabidiol (CBD), for The tricyclic antidepressant amitriptyline (AT) approved use pain-related syndromes, alone within approach. Therefore, we investigated sex- dosedependent effects CBD AT antinociception the 2.5% formalin inflammatory...